CAS1_CUCPE
ID CAS1_CUCPE Reviewed; 766 AA.
AC Q6BE25;
DT 19-OCT-2011, integrated into UniProtKB/Swiss-Prot.
DT 13-SEP-2004, sequence version 1.
DT 25-MAY-2022, entry version 51.
DE RecName: Full=Cycloartenol synthase;
DE EC=5.4.99.8;
GN Name=CPX;
OS Cucurbita pepo (Vegetable marrow) (Summer squash).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Cucurbitales; Cucurbitaceae; Cucurbiteae; Cucurbita.
OX NCBI_TaxID=3663;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND CATALYTIC ACTIVITY.
RX DOI=10.1016/j.tet.2004.04.088;
RA Shibuya M., Adachi S., Ebizuka Y.;
RT "Cucurbitadienol synthase, the first committed enzyme for cucurbitacin
RT biosynthesis, is a distinct enzyme from cycloartenol synthase for
RT phytosterol biosynthesis.";
RL Tetrahedron 60:6995-7003(2004).
CC -!- FUNCTION: Oxidosqualene cyclase involved in the biosynthesis of
CC cycloartenol. {ECO:0000269|Ref.1}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(S)-2,3-epoxysqualene = cycloartenol; Xref=Rhea:RHEA:21308,
CC ChEBI:CHEBI:15441, ChEBI:CHEBI:17030; EC=5.4.99.8;
CC Evidence={ECO:0000269|Ref.1};
CC -!- SIMILARITY: Belongs to the terpene cyclase/mutase family.
CC {ECO:0000305}.
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DR EMBL; AB116237; BAD34644.1; -; mRNA.
DR AlphaFoldDB; Q6BE25; -.
DR SMR; Q6BE25; -.
DR GO; GO:0005811; C:lipid droplet; IEA:InterPro.
DR GO; GO:0016871; F:cycloartenol synthase activity; IDA:UniProtKB.
DR GO; GO:0016104; P:triterpenoid biosynthetic process; IEA:InterPro.
DR CDD; cd02892; SQCY_1; 1.
DR InterPro; IPR032696; SQ_cyclase_C.
DR InterPro; IPR032697; SQ_cyclase_N.
DR InterPro; IPR018333; Squalene_cyclase.
DR InterPro; IPR002365; Terpene_synthase_CS.
DR InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR PANTHER; PTHR11764; PTHR11764; 1.
DR Pfam; PF13243; SQHop_cyclase_C; 1.
DR Pfam; PF13249; SQHop_cyclase_N; 1.
DR SFLD; SFLDG01016; Prenyltransferase_Like_2; 1.
DR SUPFAM; SSF48239; SSF48239; 2.
DR TIGRFAMs; TIGR01787; squalene_cyclas; 1.
DR PROSITE; PS01074; TERPENE_SYNTHASES; 1.
PE 1: Evidence at protein level;
KW Isomerase; Repeat.
FT CHAIN 1..766
FT /note="Cycloartenol synthase"
FT /id="PRO_0000412985"
FT REPEAT 155..196
FT /note="PFTB 1"
FT REPEAT 520..565
FT /note="PFTB 2"
FT REPEAT 597..637
FT /note="PFTB 3"
FT REPEAT 646..687
FT /note="PFTB 4"
FT ACT_SITE 491
FT /note="Proton donor"
FT /evidence="ECO:0000250|UniProtKB:P48449"
SQ SEQUENCE 766 AA; 87364 MW; C01270A357B60007 CRC64;
MWQLKIGADT VPSDPSNAGG WLSTLNNHVG RQVWHFHPEL GSPEDLQQIQ QARQHFSDHR
FEKKHSADLL MRMQFAKENS SFVNLPQVKV KDKEDVTEEA VTRTLRRAIN FYSTIQADDG
HWPGDYGGPM FLIPGLVITL SITGALNAVL STEHQREICR YLYNHQNKDG GWGLHIEGPS
TMFGSVLNYV TLRLLGEEAE DGQGAVDKAR KWILDHGGAA AITSWGKMWL SVLGVYEWAG
NNPLPPELWL LPYLLPCHPG RMWCHCRMVY LPMCYLYGKR FVGPITPIIR SLRKELYLVP
YHEVDWNKAR NQCAKEDLYY PHPLVQDILW ATLHHVYEPL FMHWPAKRLR EKALQSVMQH
IHYEDENTRY ICIGPVNKVL NMLCCWAEDP HSEAFKLHIP RIYDYLWIAE DGMKMQGYNG
SQLWDTAFAV QAIISTELAE EYETTLRKAH KYIKDSQVLE DCPGDLQSWY RHISKGAWPF
STADHGWPIS DCTAEGLKAV LLLSKLPSEI VGKSIDEQQL YNAVNVILSL QNTDGGFATY
ELTRSYRWLE LMNPAETFGD IVIDYPYVEC SSAAIQALAA FKKLYPGHRR DEIDNCIAEA
ADFIESIQAT DGSWYGSWGV CFTYGGWFGI RGLVAAGRRY NNCSSLRKAC DFLLSKELAA
GGWGESYLSC QNKVYTNIKD DRPHIVNTGW AMLSLIDAGQ SERDPTPLHR AARVLINSQM
EDGDFPQEEI MGVFNKNCMI SYSAYRNIFP IWALGEYRSR VLKPLK