CAS1_DICDI
ID CAS1_DICDI Reviewed; 703 AA.
AC Q55D85; Q9NDE9;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 25-MAY-2022, entry version 97.
DE RecName: Full=Cycloartenol synthase;
DE EC=5.4.99.8;
DE AltName: Full=2,3-epoxysqualene--cycloartenol cyclase;
DE AltName: Full=Oxidosqualene cyclase;
GN Name=cas1; ORFNames=DDB_G0269226;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND CATALYTIC ACTIVITY.
RX PubMed=10783001; DOI=10.1007/s11745-000-0520-3;
RA Godzina S.M., Lovato M.A., Meyer M.M., Foster K.A., Wilson W.K., Gu W.,
RA de Hostos E.L., Matsuda S.P.T.;
RT "Cloning and characterization of the Dictyostelium discoideum cycloartenol
RT synthase cDNA.";
RL Lipids 35:249-255(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- FUNCTION: Converts oxidosqualene to cycloartenol (in vitro).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(S)-2,3-epoxysqualene = cycloartenol; Xref=Rhea:RHEA:21308,
CC ChEBI:CHEBI:15441, ChEBI:CHEBI:17030; EC=5.4.99.8;
CC Evidence={ECO:0000269|PubMed:10783001};
CC -!- SIMILARITY: Belongs to the terpene cyclase/mutase family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF80384.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AF159949; AAF80384.1; ALT_INIT; mRNA.
DR EMBL; AAFI02000005; EAL71963.1; -; Genomic_DNA.
DR RefSeq; XP_646246.1; XM_641154.1.
DR AlphaFoldDB; Q55D85; -.
DR SMR; Q55D85; -.
DR STRING; 44689.DDB0191311; -.
DR PaxDb; Q55D85; -.
DR EnsemblProtists; EAL71963; EAL71963; DDB_G0269226.
DR GeneID; 8617202; -.
DR KEGG; ddi:DDB_G0269226; -.
DR dictyBase; DDB_G0269226; cas1.
DR eggNOG; KOG0497; Eukaryota.
DR HOGENOM; CLU_009074_2_1_1; -.
DR InParanoid; Q55D85; -.
DR OMA; CWARQTI; -.
DR PhylomeDB; Q55D85; -.
DR Reactome; R-DDI-191273; Cholesterol biosynthesis.
DR PRO; PR:Q55D85; -.
DR Proteomes; UP000002195; Chromosome 1.
DR GO; GO:0005811; C:lipid droplet; IBA:GO_Central.
DR GO; GO:0016871; F:cycloartenol synthase activity; IDA:dictyBase.
DR GO; GO:0000250; F:lanosterol synthase activity; IBA:GO_Central.
DR GO; GO:0006695; P:cholesterol biosynthetic process; IBA:GO_Central.
DR GO; GO:0016126; P:sterol biosynthetic process; TAS:dictyBase.
DR GO; GO:0016104; P:triterpenoid biosynthetic process; IEA:InterPro.
DR CDD; cd02892; SQCY_1; 1.
DR InterPro; IPR032696; SQ_cyclase_C.
DR InterPro; IPR032697; SQ_cyclase_N.
DR InterPro; IPR018333; Squalene_cyclase.
DR InterPro; IPR002365; Terpene_synthase_CS.
DR InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR PANTHER; PTHR11764; PTHR11764; 1.
DR Pfam; PF13243; SQHop_cyclase_C; 1.
DR Pfam; PF13249; SQHop_cyclase_N; 1.
DR SFLD; SFLDG01016; Prenyltransferase_Like_2; 1.
DR SUPFAM; SSF48239; SSF48239; 2.
DR TIGRFAMs; TIGR01787; squalene_cyclas; 1.
DR PROSITE; PS01074; TERPENE_SYNTHASES; 1.
PE 1: Evidence at protein level;
KW Isomerase; Lipid biosynthesis; Lipid metabolism; Reference proteome;
KW Repeat; Steroid biosynthesis.
FT CHAIN 1..703
FT /note="Cycloartenol synthase"
FT /id="PRO_0000327386"
FT REPEAT 59..103
FT /note="PFTB 1"
FT REPEAT 106..148
FT /note="PFTB 2"
FT REPEAT 461..503
FT /note="PFTB 3"
FT REPEAT 539..579
FT /note="PFTB 4"
FT REPEAT 587..628
FT /note="PFTB 5"
FT REPEAT 645..686
FT /note="PFTB 6"
FT ACT_SITE 435
FT /note="Proton donor"
FT /evidence="ECO:0000250|UniProtKB:P48449"
SQ SEQUENCE 703 AA; 80763 MW; C50F9F28056CA56B CRC64;
MTTTNWSLKV DRGRQTWEYS QEKKEATDVD IHLLRLKEPG THCPEGCDLN RAKTPQQAIK
KAFQYFSKVQ TEDGHWAGDY GGPMFLLPGL VITCYVTGYQ LPESTQREII RYLFNRQNPV
DGGWGLHIEA HSDIFGTTLQ YVSLRLLGVP ADHPSVVKAR TFLLQNGGAT GIPSWGKFWL
ATLNAYDWNG LNPIPIEFWL LPYNLPIAPG RWWCHCRMVY LPMSYIYAKK TTGPLTDLVK
DLRREIYCQE YEKINWSEQR NNISKLDMYY EHTSLLNVIN GSLNAYEKVH SKWLRDKAID
YTFDHIRYED EQTKYIDIGP VNKTVNMLCV WDREGKSPAF YKHADRLKDY LWLSFDGMKM
QGYNGSQLWD TAFTIQAFME SGIANQFQDC MKLAGHYLDI SQVPEDARDM KHYHRHYSKG
AWPFSTVDHG WPISDCTAEG IKSALALRSL PFIEPISLDR IADGINVLLT LQNGDGGWAS
YENTRGPKWL EKFNPSEVFQ NIMIDYSYVE CSAACIQAMS AFRKHAPNHP RIKEINRSIA
RGVKFIKSIQ RQDGSWLGSW GICFTYGTWF GIEGLVASGE PLTSPSIVKA CKFLASKQRA
DGGWGESFKS NVTKEYVQHE TSQVVNTGWA LLSLMSAKYP DRECIERGIK FLIQRQYPNG
DFPQESIIGV FNFNCMISYS NYKNIFPLWA LSRYNQLYLK SKI
