ID   CAS1_FUSNN              Reviewed;         338 AA.
AC   Q8RED1;
DT   16-MAY-2012, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   03-AUG-2022, entry version 89.
DE   RecName: Full=CRISPR-associated endonuclease Cas1 {ECO:0000255|HAMAP-Rule:MF_01470};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_01470};
GN   Name=cas1 {ECO:0000255|HAMAP-Rule:MF_01470}; OrderedLocusNames=FN1177;
OS   Fusobacterium nucleatum subsp. nucleatum (strain ATCC 25586 / DSM 15643 /
OS   BCRC 10681 / CIP 101130 / JCM 8532 / KCTC 2640 / LMG 13131 / VPI 4355).
OC   Bacteria; Fusobacteria; Fusobacteriales; Fusobacteriaceae; Fusobacterium.
OX   NCBI_TaxID=190304;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25586 / DSM 15643 / BCRC 10681 / CIP 101130 / JCM 8532 / KCTC
RC   2640 / LMG 13131 / VPI 4355;
RX   PubMed=11889109; DOI=10.1128/jb.184.7.2005-2018.2002;
RA   Kapatral V., Anderson I., Ivanova N., Reznik G., Los T., Lykidis A.,
RA   Bhattacharyya A., Bartman A., Gardner W., Grechkin G., Zhu L., Vasieva O.,
RA   Chu L., Kogan Y., Chaga O., Goltsman E., Bernal A., Larsen N., D'Souza M.,
RA   Walunas T., Pusch G., Haselkorn R., Fonstein M., Kyrpides N.C.,
RA   Overbeek R.;
RT   "Genome sequence and analysis of the oral bacterium Fusobacterium nucleatum
RT   strain ATCC 25586.";
RL   J. Bacteriol. 184:2005-2018(2002).
CC   -!- FUNCTION: CRISPR (clustered regularly interspaced short palindromic
CC       repeat), is an adaptive immune system that provides protection against
CC       mobile genetic elements (viruses, transposable elements and conjugative
CC       plasmids). CRISPR clusters contain spacers, sequences complementary to
CC       antecedent mobile elements, and target invading nucleic acids. CRISPR
CC       clusters are transcribed and processed into CRISPR RNA (crRNA). Acts as
CC       a dsDNA endonuclease. Involved in the integration of spacer DNA into
CC       the CRISPR cassette. {ECO:0000255|HAMAP-Rule:MF_01470}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01470};
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01470};
CC   -!- SUBUNIT: Homodimer, forms a heterotetramer with a Cas2 homodimer.
CC       {ECO:0000255|HAMAP-Rule:MF_01470}.
CC   -!- SIMILARITY: Belongs to the CRISPR-associated endonuclease Cas1 family.
CC       {ECO:0000255|HAMAP-Rule:MF_01470}.
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DR   EMBL; AE009951; AAL95373.1; -; Genomic_DNA.
DR   RefSeq; NP_604074.1; NC_003454.1.
DR   AlphaFoldDB; Q8RED1; -.
DR   SMR; Q8RED1; -.
DR   STRING; 190304.FN1177; -.
DR   EnsemblBacteria; AAL95373; AAL95373; FN1177.
DR   KEGG; fnu:FN1177; -.
DR   PATRIC; fig|190304.8.peg.1741; -.
DR   eggNOG; COG1518; Bacteria.
DR   HOGENOM; CLU_052779_2_0_0; -.
DR   InParanoid; Q8RED1; -.
DR   OMA; AYKLIKH; -.
DR   BioCyc; FNUC190304:G1FZS-1755-MON; -.
DR   Proteomes; UP000002521; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0004520; F:endodeoxyribonuclease activity; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-UniRule.
DR   GO; GO:0043571; P:maintenance of CRISPR repeat elements; IEA:UniProtKB-UniRule.
DR   CDD; cd09722; Cas1_I-B; 1.
DR   Gene3D; 1.20.120.920; -; 1.
DR   Gene3D; 3.100.10.20; -; 1.
DR   HAMAP; MF_01470; Cas1; 1.
DR   InterPro; IPR002729; CRISPR-assoc_Cas1.
DR   InterPro; IPR042206; CRISPR-assoc_Cas1_C.
DR   InterPro; IPR019858; CRISPR-assoc_Cas1_HMARI/TNEAP.
DR   InterPro; IPR042211; CRISPR-assoc_Cas1_N.
DR   PANTHER; PTHR43219; PTHR43219; 1.
DR   Pfam; PF01867; Cas_Cas1; 1.
DR   TIGRFAMs; TIGR00287; cas1; 1.
DR   TIGRFAMs; TIGR03641; cas1_HMARI; 1.
PE   3: Inferred from homology;
KW   Antiviral defense; DNA-binding; Endonuclease; Hydrolase; Magnesium;
KW   Manganese; Metal-binding; Nuclease; Reference proteome.
FT   CHAIN           1..338
FT                   /note="CRISPR-associated endonuclease Cas1"
FT                   /id="PRO_0000417077"
FT   BINDING         165
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01470"
FT   BINDING         230
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01470"
FT   BINDING         245
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01470"
SQ   SEQUENCE   338 AA;  40819 MW;  CA00AA9225E885A2 CRC64;
     MFCIGGVNMK RSFFLYSNGT LKRKDNTITF INEKDEKRDI PIEMVDDFYV MSEMNFNTKF
     INYISQFGIP IHFFNYYTFY TGSFYPREMN ISGQLLVKQV EHYTNEQKRV EIAREFIEGA
     SFNIYRNLRY YNGRGKDLKL YMDQIEELRK HLKEVNNVEE LMGYEGNIRK IYYEAWNIIV
     NQEIDFEKRV KNPPDNMINS LISFVNTLFY TKVLGEIYKT QLNPTVSYLH QPSTRRFSLS
     LDISEVFKPL IVDRLIFSLL NKNQITEKSF VKDFEYLRLK EDVSKLIVQE FEDRLKQIIT
     HKDLNRKISY QYLVRLECYK LIKHLLGEKK YKSFQMWW