ID   CAS1_GLYGL              Reviewed;         757 AA.
AC   Q9SXV6;
DT   16-NOV-2011, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   25-MAY-2022, entry version 65.
DE   RecName: Full=Cycloartenol synthase;
DE            EC=5.4.99.8;
GN   Name=GgCAS1;
OS   Glycyrrhiza glabra (Licorice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; Hologalegina; IRL clade; Galegeae; Glycyrrhiza.
OX   NCBI_TaxID=49827;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND CATALYTIC ACTIVITY.
RX   PubMed=10706391; DOI=10.1248/bpb.23.231;
RA   Hayashi H., Hiraoka N., Ikeshiro Y., Kushiro T., Morita M., Shibuya M.,
RA   Ebizuka Y.;
RT   "Molecular cloning and characterization of a cDNA for Glycyrrhiza glabra
RT   cycloartenol synthase.";
RL   Biol. Pharm. Bull. 23:231-234(2000).
RN   [2]
RP   TISSUE SPECIFICITY, INDUCTION, AND DEVELOPMENTAL STAGE.
RX   PubMed=15256745; DOI=10.1248/bpb.27.1086;
RA   Hayashi H., Huang P., Takada S., Obinata M., Inoue K., Shibuya M.,
RA   Ebizuka Y.;
RT   "Differential expression of three oxidosqualene cyclase mRNAs in
RT   Glycyrrhiza glabra.";
RL   Biol. Pharm. Bull. 27:1086-1092(2004).
CC   -!- FUNCTION: Oxidosqualene cyclase converting oxidosqualene to
CC       cycloartenol. Required for the production of sterols.
CC       {ECO:0000269|PubMed:10706391}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(S)-2,3-epoxysqualene = cycloartenol; Xref=Rhea:RHEA:21308,
CC         ChEBI:CHEBI:15441, ChEBI:CHEBI:17030; EC=5.4.99.8;
CC         Evidence={ECO:0000269|PubMed:10706391};
CC   -!- TISSUE SPECIFICITY: Expressed in thickened roots and root nodules.
CC       {ECO:0000269|PubMed:15256745}.
CC   -!- DEVELOPMENTAL STAGE: Constant levels of expression during germination
CC       and throughout the year. {ECO:0000269|PubMed:15256745}.
CC   -!- INDUCTION: No effect of methyl jasmonate (MeJA) or gibberellin A3
CC       (GA3). {ECO:0000269|PubMed:15256745}.
CC   -!- SIMILARITY: Belongs to the terpene cyclase/mutase family.
CC       {ECO:0000305}.
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DR   EMBL; AB025968; BAA76902.1; -; mRNA.
DR   AlphaFoldDB; Q9SXV6; -.
DR   SMR; Q9SXV6; -.
DR   BRENDA; 5.4.99.8; 2487.
DR   GO; GO:0005811; C:lipid droplet; IEA:InterPro.
DR   GO; GO:0016871; F:cycloartenol synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016104; P:triterpenoid biosynthetic process; IEA:InterPro.
DR   CDD; cd02892; SQCY_1; 1.
DR   InterPro; IPR032696; SQ_cyclase_C.
DR   InterPro; IPR032697; SQ_cyclase_N.
DR   InterPro; IPR018333; Squalene_cyclase.
DR   InterPro; IPR002365; Terpene_synthase_CS.
DR   InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR   PANTHER; PTHR11764; PTHR11764; 1.
DR   Pfam; PF13243; SQHop_cyclase_C; 1.
DR   Pfam; PF13249; SQHop_cyclase_N; 1.
DR   SFLD; SFLDG01016; Prenyltransferase_Like_2; 1.
DR   SUPFAM; SSF48239; SSF48239; 2.
DR   TIGRFAMs; TIGR01787; squalene_cyclas; 1.
DR   PROSITE; PS01074; TERPENE_SYNTHASES; 1.
PE   1: Evidence at protein level;
KW   Isomerase; Repeat.
FT   CHAIN           1..757
FT                   /note="Cycloartenol synthase"
FT                   /id="PRO_0000413992"
FT   REPEAT          147..188
FT                   /note="PFTB 1"
FT   REPEAT          512..557
FT                   /note="PFTB 2"
FT   REPEAT          589..629
FT                   /note="PFTB 3"
FT   REPEAT          638..679
FT                   /note="PFTB 4"
FT   REPEAT          700..741
FT                   /note="PFTB 5"
FT   ACT_SITE        483
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250|UniProtKB:P48449"
SQ   SEQUENCE   757 AA;  86454 MW;  396BBAEB1C2A2447 CRC64;
     MWKLKIAEGG SPWLRTVNNH VGRQVWEFDP KLGSPEDLLE IEKARQNFHD NRFTHKHSAD
     LLMRIHFAKE NPMNEVLPKV RVKDIEDVTE ETVKTTLRRA INFHSTLQSH DGHWPGDYGG
     PMFLMPGLVI TLSITGALNA VLTEEHRKEI CRYLYNHQNK DGGWGLHIEG PSTMFGSVLN
     YVALRLLGEG PNDRQGEMEK GRDWILGHGG ATFITSWGKM WLSVLGVYEW SGNNPLPPEI
     WLLPYVLPIH PGRMWCHCRM VYLPMSYLYG KRFVGPITPT ILSLRKELYT IPYHDIDWNQ
     ARNLCAKEDL YYPHPLVQDI LWASLHKFLE PILMHWPGKK LREMAIKTAI EHIHYEDDNT
     RYLCIGPVNK VLNMLCCWVE DPNSEAFKLH LPRIYDYLWI AEDGMKMQGY NGSQLWDTAF
     TAQAIISSNL IEEYGPTLRK AHTYIKNSQV LEDCPGDLSK WYRHISKGAW PFSTADHGWP
     ISDCTAEGLK AVLLLSKIAP EIVGEPLDAK RLYDAVNVIL SLQNEDGGFA TYELTRSYTW
     LELINPAETF GDIVIDYPYV ECTSAAIQAL TSFKKLYPGH RREEIQCCIE KAASFIEKTQ
     ASDGSWYGSW GVCFTYGTWF GVKGLIAAGK SFNNCSSIRK ACEFLLSKQL PSGGWGESYL
     SCQNKVYSNV ESNRSHVVNT GWAMLALIDA EQAKRDPTPL HRAAVYLINS QMENGDFPQQ
     EIMGVFNKNC MITYAAYRNV FPIWALGEYR HRVLQSQ