CAS1_HALVD
ID CAS1_HALVD Reviewed; 331 AA.
AC D4GQP0;
DT 04-MAR-2015, integrated into UniProtKB/Swiss-Prot.
DT 18-MAY-2010, sequence version 1.
DT 03-AUG-2022, entry version 54.
DE RecName: Full=CRISPR-associated endonuclease Cas1 {ECO:0000255|HAMAP-Rule:MF_01470};
DE EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_01470};
GN Name=cas1 {ECO:0000255|HAMAP-Rule:MF_01470}; OrderedLocusNames=HVO_A0211;
OS Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 /
OS NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii).
OG Plasmid pHV4.
OC Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Haloferacales;
OC Haloferacaceae; Haloferax.
OX NCBI_TaxID=309800;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM
RC B-1768 / DS2 {ECO:0000312|Proteomes:UP000008243};
RX PubMed=20333302; DOI=10.1371/journal.pone.0009605;
RA Hartman A.L., Norais C., Badger J.H., Delmas S., Haldenby S., Madupu R.,
RA Robinson J., Khouri H., Ren Q., Lowe T.M., Maupin-Furlow J.,
RA Pohlschroder M., Daniels C., Pfeiffer F., Allers T., Eisen J.A.;
RT "The complete genome sequence of Haloferax volcanii DS2, a model
RT archaeon.";
RL PLoS ONE 5:E9605-E9605(2010).
RN [2]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=DS2 / DS70 / H26;
RX PubMed=22767603; DOI=10.1074/jbc.m112.377002;
RA Fischer S., Maier L.K., Stoll B., Brendel J., Fischer E., Pfeiffer F.,
RA Dyall-Smith M., Marchfelder A.;
RT "An archaeal immune system can detect multiple protospacer adjacent motifs
RT (PAMs) to target invader DNA.";
RL J. Biol. Chem. 287:33351-33363(2012).
CC -!- FUNCTION: CRISPR (clustered regularly interspaced short palindromic
CC repeat), is an adaptive immune system that provides protection against
CC mobile genetic elements (viruses, transposable elements and conjugative
CC plasmids). CRISPR clusters contain spacers, sequences complementary to
CC antecedent mobile elements, and target invading nucleic acids. CRISPR
CC clusters are transcribed and processed into CRISPR RNA (crRNA). Acts as
CC a dsDNA endonuclease. Involved in the integration of spacer DNA into
CC the CRISPR cassette. Plasmid targeted by CRISPR locus P1 transform
CC wild-type cells very poorly (PubMed:22767603). {ECO:0000255|HAMAP-
CC Rule:MF_01470, ECO:0000269|PubMed:22767603}.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01470};
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01470};
CC -!- SUBUNIT: Homodimer, forms a heterotetramer with a Cas2 homodimer.
CC {ECO:0000255|HAMAP-Rule:MF_01470}.
CC -!- DISRUPTION PHENOTYPE: Loss of the 8 Cas genes in this locus (cas1,
CC cas2, cas3, cas4, cas5, cas6, cas7 and cas8b) leads to loss of CRISPR
CC interference against plasmid targeted by this CRISPR locus, i.e.
CC plasmid is not destroyed by CRISPR. {ECO:0000269|PubMed:22767603}.
CC -!- MISCELLANEOUS: There are 3 CRISPR RNA loci in this organism and a
CC single cas gene locus. A CRISPR-Cas type I-B system.
CC {ECO:0000303|PubMed:20333302, ECO:0000303|PubMed:22767603}.
CC -!- SIMILARITY: Belongs to the CRISPR-associated endonuclease Cas1 family.
CC {ECO:0000255|HAMAP-Rule:MF_01470}.
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DR EMBL; CP001955; ADE01744.1; -; Genomic_DNA.
DR RefSeq; WP_013035043.1; NZ_AOHU01000052.1.
DR AlphaFoldDB; D4GQP0; -.
DR SMR; D4GQP0; -.
DR STRING; 309800.C498_09736; -.
DR EnsemblBacteria; ADE01744; ADE01744; HVO_A0211.
DR GeneID; 8923750; -.
DR KEGG; hvo:HVO_A0211; -.
DR eggNOG; arCOG01452; Archaea.
DR HOGENOM; CLU_052779_2_0_2; -.
DR OMA; AYKLIKH; -.
DR OrthoDB; 70037at2157; -.
DR Proteomes; UP000008243; Plasmid pHV4.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0004520; F:endodeoxyribonuclease activity; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-UniRule.
DR GO; GO:0043571; P:maintenance of CRISPR repeat elements; IEA:UniProtKB-UniRule.
DR CDD; cd09722; Cas1_I-B; 1.
DR Gene3D; 1.20.120.920; -; 1.
DR Gene3D; 3.100.10.20; -; 1.
DR HAMAP; MF_01470; Cas1; 1.
DR InterPro; IPR002729; CRISPR-assoc_Cas1.
DR InterPro; IPR042206; CRISPR-assoc_Cas1_C.
DR InterPro; IPR019858; CRISPR-assoc_Cas1_HMARI/TNEAP.
DR InterPro; IPR042211; CRISPR-assoc_Cas1_N.
DR PANTHER; PTHR43219; PTHR43219; 1.
DR Pfam; PF01867; Cas_Cas1; 1.
DR TIGRFAMs; TIGR00287; cas1; 1.
DR TIGRFAMs; TIGR03641; cas1_HMARI; 1.
PE 3: Inferred from homology;
KW Antiviral defense; DNA-binding; Endonuclease; Hydrolase; Magnesium;
KW Manganese; Metal-binding; Nuclease; Plasmid; Reference proteome.
FT CHAIN 1..331
FT /note="CRISPR-associated endonuclease Cas1"
FT /id="PRO_0000432148"
FT BINDING 158
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01470"
FT BINDING 223
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01470"
FT BINDING 238
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01470"
SQ SEQUENCE 331 AA; 38197 MW; C2AB225877BB24AE CRC64;
MTKANHHIFA DGELSRKEGT LRIDTLEGET KYLPVESIDA LYLHGQISFN TRALGLLNKH
GVPVHIFGWK DYYRGSYLPK RSQLSGNTVV EQVRAYDNTE RRLRIGHRII EASIHNMRAN
LQYYSGRRGD FDSVVETLRE LKTAVSDTQR IDELRAVEGD ARKRYYDCFD SILEAPFRLA
KREYNPPSNE TNALISFLNG MVYTSCVSAI RKTALDPTVG FVHEPGERRF TLSLDIADIF
KPILADRLVF RLVNRKQITT DDFETELAGC LLTEQGRLTV LEEFERSLDQ TVQHPRLKRK
VSFKTLIQTD VYSLKKHLLT GEPYHATEKW W
