ID   CAS1_KORCO              Reviewed;         341 AA.
AC   B1L400;
DT   16-MAY-2012, integrated into UniProtKB/Swiss-Prot.
DT   29-APR-2008, sequence version 1.
DT   03-AUG-2022, entry version 65.
DE   RecName: Full=CRISPR-associated endonuclease Cas1 {ECO:0000255|HAMAP-Rule:MF_01470};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_01470};
GN   Name=cas1 {ECO:0000255|HAMAP-Rule:MF_01470}; OrderedLocusNames=Kcr_0423;
OS   Korarchaeum cryptofilum (strain OPF8).
OC   Archaea; Candidatus Korarchaeota; Candidatus Korarchaeum.
OX   NCBI_TaxID=374847;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=OPF8;
RX   PubMed=18535141; DOI=10.1073/pnas.0801980105;
RA   Elkins J.G., Podar M., Graham D.E., Makarova K.S., Wolf Y., Randau L.,
RA   Hedlund B.P., Brochier-Armanet C., Kunin V., Anderson I., Lapidus A.,
RA   Goltsman E., Barry K., Koonin E.V., Hugenholtz P., Kyrpides N., Wanner G.,
RA   Richardson P., Keller M., Stetter K.O.;
RT   "A korarchaeal genome reveals new insights into the evolution of the
RT   Archaea.";
RL   Proc. Natl. Acad. Sci. U.S.A. 105:8102-8107(2008).
CC   -!- FUNCTION: CRISPR (clustered regularly interspaced short palindromic
CC       repeat), is an adaptive immune system that provides protection against
CC       mobile genetic elements (viruses, transposable elements and conjugative
CC       plasmids). CRISPR clusters contain spacers, sequences complementary to
CC       antecedent mobile elements, and target invading nucleic acids. CRISPR
CC       clusters are transcribed and processed into CRISPR RNA (crRNA). Acts as
CC       a dsDNA endonuclease. Involved in the integration of spacer DNA into
CC       the CRISPR cassette. {ECO:0000255|HAMAP-Rule:MF_01470}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01470};
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01470};
CC   -!- SUBUNIT: Homodimer, forms a heterotetramer with a Cas2 homodimer.
CC       {ECO:0000255|HAMAP-Rule:MF_01470}.
CC   -!- SIMILARITY: Belongs to the CRISPR-associated endonuclease Cas1 family.
CC       {ECO:0000255|HAMAP-Rule:MF_01470}.
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DR   EMBL; CP000968; ACB07179.1; -; Genomic_DNA.
DR   RefSeq; WP_012309076.1; NC_010482.1.
DR   AlphaFoldDB; B1L400; -.
DR   SMR; B1L400; -.
DR   STRING; 374847.Kcr_0423; -.
DR   PRIDE; B1L400; -.
DR   EnsemblBacteria; ACB07179; ACB07179; Kcr_0423.
DR   GeneID; 6093710; -.
DR   KEGG; kcr:Kcr_0423; -.
DR   eggNOG; arCOG01452; Archaea.
DR   HOGENOM; CLU_052779_0_0_2; -.
DR   InParanoid; B1L400; -.
DR   OMA; YYVGSFY; -.
DR   OrthoDB; 70037at2157; -.
DR   PhylomeDB; B1L400; -.
DR   Proteomes; UP000001686; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-UniRule.
DR   GO; GO:0043571; P:maintenance of CRISPR repeat elements; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.20.120.920; -; 1.
DR   Gene3D; 3.100.10.20; -; 1.
DR   HAMAP; MF_01470; Cas1; 1.
DR   InterPro; IPR002729; CRISPR-assoc_Cas1.
DR   InterPro; IPR042206; CRISPR-assoc_Cas1_C.
DR   InterPro; IPR042211; CRISPR-assoc_Cas1_N.
DR   Pfam; PF01867; Cas_Cas1; 1.
DR   TIGRFAMs; TIGR00287; cas1; 1.
PE   3: Inferred from homology;
KW   Antiviral defense; DNA-binding; Endonuclease; Hydrolase; Magnesium;
KW   Manganese; Metal-binding; Nuclease; Reference proteome.
FT   CHAIN           1..341
FT                   /note="CRISPR-associated endonuclease Cas1"
FT                   /id="PRO_0000417096"
FT   BINDING         173
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01470"
FT   BINDING         242
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01470"
FT   BINDING         257
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01470"
SQ   SEQUENCE   341 AA;  39291 MW;  547418BAE072F614 CRC64;
     METTQYGHLL IDGFGVSLRK RRGRILILSK GEKKEIPMKS VKEVVIIGKA ALSSELLKAL
     AQSGTDLLIA TPTGRPVARL IPAKAGGTAR NRYEQYKSLE DRRGIEIARA VIVGKIRNQA
     SNLSYYSKAR RMDEELSSEL YDAAQQLKRE MEELKNEEFP DIDEARKRIM ARESKCANIY
     WEKIASIMEE WKFRGREKRT DLEGNVIDPV NLCLNVCYNL LSAQIWKNVL RFGLDPFLGY
     LHVERPGRIS LVYDLMEPFR PMVDRFVFSY LRGMSPSLFS SNIVSGTIAS LRSRFFSDFM
     NWRLDYKGRK LGMETIMFLY VRDIVSFLRG GKEPTMPYIP W