ID   CAS1_LUFAE              Reviewed;         765 AA.
AC   Q9SLP9;
DT   19-OCT-2011, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   25-MAY-2022, entry version 63.
DE   RecName: Full=Cycloartenol synthase;
DE            Short=LcCAS1;
DE            EC=5.4.99.8;
GN   Name=CAS1;
OS   Luffa aegyptiaca (Sponge gourd) (Luffa cylindrica).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Cucurbitales; Cucurbitaceae; Sicyoeae; Luffa.
OX   NCBI_TaxID=3670;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND CATALYTIC ACTIVITY.
RA   Hayashi H., Hiraoka N., Ikeshiro Y., Yazaki K., Tanaka S., Kushiro T.,
RA   Shibuya M., Ebizuka Y.;
RT   "Molecular cloning of a cDNA encoding cycloartenol synthase from Luffa
RT   cylindrica.";
RL   (er) Plant Gene Register PGR99-183(1999).
RN   [2]
RP   INDUCTION.
RX   PubMed=11733028; DOI=10.1046/j.0014-2956.2001.02588.x;
RA   Hayashi H., Huang P., Inoue K., Hiraoka N., Ikeshiro Y., Yazaki K.,
RA   Tanaka S., Kushiro T., Shibuya M., Ebizuka Y.;
RT   "Molecular cloning and characterization of isomultiflorenol synthase, a new
RT   triterpene synthase from Luffa cylindrica, involved in biosynthesis of
RT   bryonolic acid.";
RL   Eur. J. Biochem. 268:6311-6317(2001).
CC   -!- FUNCTION: Oxidosqualene cyclase involved in the biosynthesis of
CC       cycloartenol. {ECO:0000269|Ref.1}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(S)-2,3-epoxysqualene = cycloartenol; Xref=Rhea:RHEA:21308,
CC         ChEBI:CHEBI:15441, ChEBI:CHEBI:17030; EC=5.4.99.8;
CC         Evidence={ECO:0000269|Ref.1};
CC   -!- MISCELLANEOUS: In cultured cells, CAS1 mRNA expression is high between
CC       days 4 and 8 and decreases at later growth stages.
CC   -!- SIMILARITY: Belongs to the terpene cyclase/mutase family.
CC       {ECO:0000305}.
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DR   EMBL; AB033334; BAA85266.1; -; mRNA.
DR   AlphaFoldDB; Q9SLP9; -.
DR   SMR; Q9SLP9; -.
DR   GO; GO:0005811; C:lipid droplet; IEA:InterPro.
DR   GO; GO:0016871; F:cycloartenol synthase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016104; P:triterpenoid biosynthetic process; IEA:InterPro.
DR   CDD; cd02892; SQCY_1; 1.
DR   InterPro; IPR032696; SQ_cyclase_C.
DR   InterPro; IPR032697; SQ_cyclase_N.
DR   InterPro; IPR018333; Squalene_cyclase.
DR   InterPro; IPR002365; Terpene_synthase_CS.
DR   InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR   PANTHER; PTHR11764; PTHR11764; 1.
DR   Pfam; PF13243; SQHop_cyclase_C; 1.
DR   Pfam; PF13249; SQHop_cyclase_N; 1.
DR   SFLD; SFLDG01016; Prenyltransferase_Like_2; 1.
DR   SUPFAM; SSF48239; SSF48239; 2.
DR   TIGRFAMs; TIGR01787; squalene_cyclas; 1.
DR   PROSITE; PS01074; TERPENE_SYNTHASES; 1.
PE   1: Evidence at protein level;
KW   Isomerase; Repeat.
FT   CHAIN           1..765
FT                   /note="Cycloartenol synthase"
FT                   /id="PRO_0000412992"
FT   REPEAT          155..196
FT                   /note="PFTB 1"
FT   REPEAT          520..565
FT                   /note="PFTB 2"
FT   REPEAT          597..637
FT                   /note="PFTB 3"
FT   REPEAT          646..687
FT                   /note="PFTB 4"
FT   ACT_SITE        491
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250|UniProtKB:P48449"
SQ   SEQUENCE   765 AA;  87146 MW;  0CEC82DA389EF3C7 CRC64;
     MWQLKIGADT VPADPSNAGG WLSSLNNHVG RQVWHFHPEL GTPEDLQQIQ HARQRFSDHR
     FEKKHSADLL MRMQFAKNNS SFVNLPQIKV KDKEDVTEEA VSRTLRRAIN FYSTIQGDDG
     HWPGDYGGPM FLIPGLVITL SITGALNAVL STEHQREICR YLYNHQNKDG GWGLHIEGPS
     TMFGSVLNYV SLRLLGEEAE DGQGAVDKAR KWILDHGGAS AITSWGKMWL SVLGVYEWAG
     NNPLPPELWL LPYLLPFHPG RMWCHCRMVY LPMCYLYGKR FVGPITPIIR SLRKELYLVP
     YHEVDWNKAR NECAKEDLYY PHPLVQDIVW ASLHHVYEPL FMRWPAKRLR EKALQCVMQH
     IHYEDENTRY ICIGPVNKVL NMLCCWVEDP HSEAFKLHIP RIFDYLWIAE DGMKMQGYNG
     SQLWDTAFAV QAIMSTKLAE EYGTTLRKAH KYIKDSQVLE DCPGDLQSWY RHISKGAWPF
     STADHGWPIS DCTAEGLKAV LLLSKLPSEI VGKSIDEEQI YDAVNVILSL QNTDGGFATY
     ELTRSYPWLE LMNPAETFGD IVIDYTYVEC TSAAIQALVA FKKLYPGHRR DEIDNCVAKA
     ADFIESIQAT DGSWYGSWGV CFTYGGWFGI RGLVAAGRRY DNCSSLRKAC DFLLSKELAS
     GGWGESYLSG QNKVYTNIKD DRPHIVNTGW AMLSLIDAGQ SERDPTPLHR AARILINSQM
     DDGDFPQEEI MGIFNKNCMI SYAAYRNIFP IWALGEYRCR VLQAP