Y4539_ARATH
ID Y4539_ARATH Reviewed; 651 AA.
AC Q9STJ8; Q3E7L1;
DT 11-JAN-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=Receptor-like serine/threonine-protein kinase At4g25390;
DE EC=2.7.11.1;
DE Flags: Precursor;
GN OrderedLocusNames=At4g25390; ORFNames=T30C3.60;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-
CC [protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.1;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-
CC threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060,
CC Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216;
CC EC=2.7.11.1;
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Single-pass type I
CC membrane protein {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9STJ8-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9STJ8-2; Sequence=VSP_040370;
CC -!- SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein
CC kinase family. {ECO:0000255|PROSITE-ProRule:PRU00159}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AK229164; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AL079350; CAB45516.1; -; Genomic_DNA.
DR EMBL; AL161563; CAB81350.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE85051.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE85052.1; -; Genomic_DNA.
DR EMBL; AK229164; -; NOT_ANNOTATED_CDS; mRNA.
DR PIR; T10219; T10219.
DR RefSeq; NP_194269.1; NM_118671.3. [Q9STJ8-1]
DR RefSeq; NP_849442.1; NM_179111.1. [Q9STJ8-2]
DR AlphaFoldDB; Q9STJ8; -.
DR SMR; Q9STJ8; -.
DR BioGRID; 13929; 1.
DR STRING; 3702.AT4G25390.1; -.
DR iPTMnet; Q9STJ8; -.
DR PaxDb; Q9STJ8; -.
DR PRIDE; Q9STJ8; -.
DR ProteomicsDB; 243000; -. [Q9STJ8-1]
DR EnsemblPlants; AT4G25390.1; AT4G25390.1; AT4G25390. [Q9STJ8-1]
DR EnsemblPlants; AT4G25390.2; AT4G25390.2; AT4G25390. [Q9STJ8-2]
DR GeneID; 828642; -.
DR Gramene; AT4G25390.1; AT4G25390.1; AT4G25390. [Q9STJ8-1]
DR Gramene; AT4G25390.2; AT4G25390.2; AT4G25390. [Q9STJ8-2]
DR KEGG; ath:AT4G25390; -.
DR Araport; AT4G25390; -.
DR TAIR; locus:2138014; AT4G25390.
DR eggNOG; KOG1187; Eukaryota.
DR HOGENOM; CLU_011728_0_0_1; -.
DR InParanoid; Q9STJ8; -.
DR OMA; WISMDEE; -.
DR OrthoDB; 337129at2759; -.
DR PhylomeDB; Q9STJ8; -.
DR PRO; PR:Q9STJ8; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q9STJ8; baseline and differential.
DR Genevisible; Q9STJ8; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0106310; F:protein serine kinase activity; IEA:RHEA.
DR GO; GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW.
DR GO; GO:0006468; P:protein phosphorylation; IEA:InterPro.
DR InterPro; IPR044576; At4g25390-like.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR000719; Prot_kinase_dom.
DR InterPro; IPR017441; Protein_kinase_ATP_BS.
DR InterPro; IPR001245; Ser-Thr/Tyr_kinase_cat_dom.
DR InterPro; IPR008271; Ser/Thr_kinase_AS.
DR PANTHER; PTHR46821; PTHR46821; 1.
DR Pfam; PF07714; PK_Tyr_Ser-Thr; 1.
DR Pfam; PF00069; Pkinase; 1.
DR SMART; SM00220; S_TKc; 1.
DR SUPFAM; SSF56112; SSF56112; 1.
DR PROSITE; PS00107; PROTEIN_KINASE_ATP; 1.
DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
DR PROSITE; PS00108; PROTEIN_KINASE_ST; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; ATP-binding; Cell membrane; Glycoprotein; Kinase;
KW Membrane; Nucleotide-binding; Receptor; Reference proteome;
KW Serine/threonine-protein kinase; Signal; Transferase; Transmembrane;
KW Transmembrane helix.
FT SIGNAL 1..25
FT /evidence="ECO:0000255"
FT CHAIN 26..651
FT /note="Receptor-like serine/threonine-protein kinase
FT At4g25390"
FT /id="PRO_0000403342"
FT TOPO_DOM 26..40
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 41..61
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 62..651
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 99..633
FT /note="Protein kinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT REGION 66..87
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 69..87
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 225
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159,
FT ECO:0000255|PROSITE-ProRule:PRU10027"
FT BINDING 105..113
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT BINDING 127
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT CARBOHYD 32
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 465..619
FT /note="RGSGSGSSIDWWLDGLSGERWLRARGNSHDSVSGEIAKSCGISSTPSMRGTV
FT CYAAPEYCNLDNNVSEKCDVYSYGVLLLVLISGRRPLEMTGSASEIQRANLMSWARKLA
FT RRGKLVDLVDQKLQNLDQEQAVLCIKVALLCLQRLPISRPSMKE -> Q (in
FT isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_040370"
FT CONFLICT 405
FT /note="W -> G (in Ref. 3; AK229164)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 651 AA; 72240 MW; EDC517150EA55878 CRC64;
MPSRSISAPV PVLAPAPIVS SLVPAAPSGH QNKTTRIFPP FVVAGAGAGF SLFITLSVCF
CKFSRKRSSP PAENASSSPR RPSPREFSYS SLRRATGSFS QANRLGQGGF GVVFRGTISG
GENVAVKVMD SGSLQGEGEF QNELFFAAKL DSPHVVPVIG FSHDRKRRRL LLVYKLMDNG
NLQDALLHRR CPELMDWNRR FLVAVNIADG IKHLHSLEPP VIHGDIKPSN VLLDSLFSAK
IADFGLARLK AEQVEISVAP ERDGDGSMVE EVESVVTTVT GYEDFNFGLV DQSPESVAKV
PGSVSASPEA TTVVSVSPEM GEKTDEDGGS VVVMKKGKES ESKDWWWKQE SNVERGRVKE
YVMQWIGSEV KKERPSRSDW IEAAALSSSS SKKLEKKTSK RLDWWLSLEE EDENKKKKKR
RMVREWWKDE YRRELAKKRK KKKKMTLEAE FCSDDGSSSV SQWRRGSGSG SSIDWWLDGL
SGERWLRARG NSHDSVSGEI AKSCGISSTP SMRGTVCYAA PEYCNLDNNV SEKCDVYSYG
VLLLVLISGR RPLEMTGSAS EIQRANLMSW ARKLARRGKL VDLVDQKLQN LDQEQAVLCI
KVALLCLQRL PISRPSMKEV LGMLKGEVNL PELPSEFSPS PPLKTTRKQR R
