ID   CAS1_MYCTU              Reviewed;         338 AA.
AC   P9WPJ5; F2GLB7; L0TDM9; P71636; Q7D6I7;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   03-AUG-2022, entry version 40.
DE   RecName: Full=CRISPR-associated endonuclease Cas1 {ECO:0000255|HAMAP-Rule:MF_01470};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_01470};
GN   Name=cas1 {ECO:0000255|HAMAP-Rule:MF_01470}; OrderedLocusNames=Rv2817c;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA   Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA   Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA   Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA   Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT   "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT   mass spectrometry.";
RL   Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
RN   [3]
RP   FUNCTION IN PLASMID RESISTANCE, AND DISRUPTION PHENOTYPE.
RC   STRAIN=H37Rv;
RX   PubMed=29979631; DOI=10.1096/fj.201800557rr;
RA   Wei W., Zhang S., Fleming J., Chen Y., Li Z., Fan S., Liu Y., Wang W.,
RA   Wang T., Liu Y., Ren B., Wang M., Jiao J., Chen Y., Zhou Y., Zhou Y.,
RA   Gu S., Zhang X., Wan L., Chen T., Zhou L., Chen Y., Zhang X.E., Li C.,
RA   Zhang H., Bi L.;
RT   "Mycobacterium tuberculosis type III-A CRISPR/Cas system crRNA and its
RT   maturation have atypical features.";
RL   FASEB J. 33:1496-1509(2019).
CC   -!- FUNCTION: CRISPR (clustered regularly interspaced short palindromic
CC       repeat) is an adaptive immune system that provides protection against
CC       mobile genetic elements (viruses, transposable elements and conjugative
CC       plasmids). CRISPR clusters contain spacers, sequences complementary to
CC       antecedent mobile elements, and target invading nucleic acids. CRISPR
CC       clusters are transcribed and processed into CRISPR RNA (crRNA). The
CC       type III-A Csm effector complex binds crRNA and acts as a crRNA-guided
CC       RNase, DNase and cyclic oligoadenylate synthase; binding of target RNA
CC       cognate to the crRNA is required for all activities (Probable). This
CC       CRISPR-Cas system protects bacteria against transformation with
CC       plasmids containing DNA homologous to its spacer regions
CC       (PubMed:29979631). {ECO:0000269|PubMed:29979631,
CC       ECO:0000305|PubMed:29979631}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01470};
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01470};
CC   -!- SUBUNIT: Homodimer, forms a heterotetramer with a Cas2 homodimer.
CC       {ECO:0000255|HAMAP-Rule:MF_01470}.
CC   -!- DISRUPTION PHENOTYPE: Deletion of the entire CRISPR-Cas locus (cas6 to
CC       cas2, Rv2824c to Rv2816c) decreases resistance to plasmids encoding
CC       spacer elements about 6-fold. {ECO:0000269|PubMed:29979631}.
CC   -!- MISCELLANEOUS: Encoded in a type III-A CRISPR locus.
CC       {ECO:0000305|PubMed:29979631}.
CC   -!- SIMILARITY: Belongs to the CRISPR-associated endonuclease Cas1 family.
CC       {ECO:0000255|HAMAP-Rule:MF_01470}.
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DR   EMBL; AL123456; CCP45617.1; -; Genomic_DNA.
DR   PIR; D70691; D70691.
DR   RefSeq; NP_217333.1; NC_000962.3.
DR   RefSeq; WP_003414279.1; NZ_NVQJ01000006.1.
DR   AlphaFoldDB; P9WPJ5; -.
DR   SMR; P9WPJ5; -.
DR   STRING; 83332.Rv2817c; -.
DR   PaxDb; P9WPJ5; -.
DR   DNASU; 888506; -.
DR   GeneID; 45426805; -.
DR   GeneID; 888506; -.
DR   KEGG; mtu:Rv2817c; -.
DR   TubercuList; Rv2817c; -.
DR   eggNOG; COG1518; Bacteria.
DR   OMA; AYKLIKH; -.
DR   PhylomeDB; P9WPJ5; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0009274; C:peptidoglycan-based cell wall; HDA:MTBBASE.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-UniRule.
DR   GO; GO:0043571; P:maintenance of CRISPR repeat elements; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.20.120.920; -; 1.
DR   Gene3D; 3.100.10.20; -; 1.
DR   HAMAP; MF_01470; Cas1; 1.
DR   InterPro; IPR002729; CRISPR-assoc_Cas1.
DR   InterPro; IPR042206; CRISPR-assoc_Cas1_C.
DR   InterPro; IPR042211; CRISPR-assoc_Cas1_N.
DR   Pfam; PF01867; Cas_Cas1; 1.
DR   TIGRFAMs; TIGR00287; cas1; 1.
PE   1: Evidence at protein level;
KW   Antiviral defense; DNA-binding; Endonuclease; Hydrolase; Magnesium;
KW   Manganese; Metal-binding; Nuclease; Reference proteome.
FT   CHAIN           1..338
FT                   /note="CRISPR-associated endonuclease Cas1"
FT                   /id="PRO_0000418217"
FT   BINDING         155
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01470"
FT   BINDING         220
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01470"
FT   BINDING         235
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01470"
SQ   SEQUENCE   338 AA;  37664 MW;  7925B1F1B947197A CRC64;
     MVQLYVSDSV SRISFADGRV IVWSEELGES QYPIETLDGI TLFGRPTMTT PFIVEMLKRE
     RDIQLFTTDG HYQGRISTPD VSYAPRLRQQ VHRTDDPAFC LSLSKRIVSR KILNQQALIR
     AHTSGQDVAE SIRTMKHSLA WVDRSGSLAE LNGFEGNAAK AYFTALGHLV PQEFAFQGRS
     TRPPLDAFNS MVSLGYSLLY KNIIGAIERH SLNAYIGFLH QDSRGHATLA SDLMEVWRAP
     IIDDTVLRLI ADGVVDTRAF SKNSDTGAVF ATREATRSIA RAFGNRIART ATYIKGDPHR
     YTFQYALDLQ LQSLVRVIEA GHPSRLVDID ITSEPSGA