ID   CAS1_NEIM8              Reviewed;         304 AA.
AC   C9X1G6;
DT   03-SEP-2014, integrated into UniProtKB/Swiss-Prot.
DT   24-NOV-2009, sequence version 1.
DT   03-AUG-2022, entry version 52.
DE   RecName: Full=CRISPR-associated endonuclease Cas1 {ECO:0000255|HAMAP-Rule:MF_01470};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_01470};
GN   Name=cas1 {ECO:0000255|HAMAP-Rule:MF_01470}; OrderedLocusNames=NMV_1994;
OS   Neisseria meningitidis serogroup C (strain 8013).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Neisseriales; Neisseriaceae;
OC   Neisseria.
OX   NCBI_TaxID=604162;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=8013;
RX   PubMed=19818133; DOI=10.1186/gb-2009-10-10-r110;
RA   Rusniok C., Vallenet D., Floquet S., Ewles H., Mouze-Soulama C., Brown D.,
RA   Lajus A., Buchrieser C., Medigue C., Glaser P., Pelicic V.;
RT   "NeMeSys: a biological resource for narrowing the gap between sequence and
RT   function in the human pathogen Neisseria meningitidis.";
RL   Genome Biol. 10:R110.1-R110.13(2009).
RN   [2]
RP   DISRUPTION PHENOTYPE.
RC   STRAIN=8013;
RX   PubMed=23706818; DOI=10.1016/j.molcel.2013.05.001;
RA   Zhang Y., Heidrich N., Ampattu B.J., Gunderson C.W., Seifert H.S.,
RA   Schoen C., Vogel J., Sontheimer E.J.;
RT   "Processing-independent CRISPR RNAs limit natural transformation in
RT   Neisseria meningitidis.";
RL   Mol. Cell 50:488-503(2013).
CC   -!- FUNCTION: CRISPR (clustered regularly interspaced short palindromic
CC       repeat), is an adaptive immune system that provides protection against
CC       mobile genetic elements (viruses, transposable elements and conjugative
CC       plasmids). CRISPR clusters contain spacers, sequences complementary to
CC       antecedent mobile elements, and target invading nucleic acids. CRISPR
CC       clusters are transcribed and processed into CRISPR RNA (crRNA). Acts as
CC       a dsDNA endonuclease. Involved in the integration of spacer DNA into
CC       the CRISPR cassette. {ECO:0000255|HAMAP-Rule:MF_01470}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01470};
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01470};
CC   -!- SUBUNIT: Homodimer, forms a heterotetramer with a Cas2 homodimer.
CC       {ECO:0000255|HAMAP-Rule:MF_01470}.
CC   -!- DISRUPTION PHENOTYPE: No effect on CRISPR interference during plasmid
CC       transformation. {ECO:0000269|PubMed:23706818}.
CC   -!- SIMILARITY: Belongs to the CRISPR-associated endonuclease Cas1 family.
CC       {ECO:0000255|HAMAP-Rule:MF_01470}.
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DR   EMBL; FM999788; CAX50780.1; -; Genomic_DNA.
DR   RefSeq; WP_002256914.1; NC_017501.1.
DR   AlphaFoldDB; C9X1G6; -.
DR   SMR; C9X1G6; -.
DR   KEGG; nmt:NMV_1994; -.
DR   PATRIC; fig|604162.3.peg.2306; -.
DR   HOGENOM; CLU_055263_1_0_4; -.
DR   OMA; DDLMEPY; -.
DR   Proteomes; UP000002076; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0004520; F:endodeoxyribonuclease activity; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-UniRule.
DR   GO; GO:0043571; P:maintenance of CRISPR repeat elements; IEA:UniProtKB-UniRule.
DR   Gene3D; 1.20.120.920; -; 1.
DR   HAMAP; MF_01470; Cas1; 1.
DR   InterPro; IPR002729; CRISPR-assoc_Cas1.
DR   InterPro; IPR042206; CRISPR-assoc_Cas1_C.
DR   InterPro; IPR019855; CRISPR-assoc_Cas1_NMENI.
DR   Pfam; PF01867; Cas_Cas1; 1.
DR   TIGRFAMs; TIGR00287; cas1; 1.
DR   TIGRFAMs; TIGR03639; cas1_NMENI; 1.
PE   3: Inferred from homology;
KW   Antiviral defense; DNA-binding; Endonuclease; Hydrolase; Magnesium;
KW   Manganese; Metal-binding; Nuclease.
FT   CHAIN           1..304
FT                   /note="CRISPR-associated endonuclease Cas1"
FT                   /id="PRO_0000429991"
FT   BINDING         148
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01470"
FT   BINDING         204
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01470"
FT   BINDING         219
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01470"
SQ   SEQUENCE   304 AA;  34471 MW;  E2D7991B7A86EB4C CRC64;
     MTWRSLLIQN GGKLSLQRRQ LLIQQNGESH TVPLEDIAVI IIENRETLIT APLLSALAEH
     GATLLTCDEQ FLPCGQWLPY AQYHRQLKIL KLQLNISEPL KKQLWQHIVR QKILNQAFVA
     DETGNDLAAK RLRTLASEVR SGDTGNREAQ AAALYFQALF GEKFTRNDNN AVNAALNYTY
     AVLRAAVART LTLYGWLPAL GLFHRSELNP FNLADDFIEP LRPLADLTVI HLYEQGRLKA
     ELTPGIKQHL IKTLHYQISI ERQHFSTLAA IDKMVSSFQA GVTDKNAKQL KLPEILPLKE
     YQYE