CAS1_PYRHO
ID CAS1_PYRHO Reviewed; 322 AA.
AC O58938;
DT 16-MAY-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1998, sequence version 1.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=CRISPR-associated endonuclease Cas1 {ECO:0000255|HAMAP-Rule:MF_01470};
DE EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_01470};
GN Name=cas1 {ECO:0000255|HAMAP-Rule:MF_01470}; OrderedLocusNames=PH1245;
OS Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC
OS 100139 / OT-3).
OC Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC Pyrococcus.
OX NCBI_TaxID=70601;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3;
RX PubMed=9679194; DOI=10.1093/dnares/5.2.55;
RA Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y., Yamamoto S.,
RA Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y., Sakai M., Ogura K.,
RA Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y., Funahashi T., Tanaka T.,
RA Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Yoshizawa T.,
RA Nakamura Y., Robb F.T., Horikoshi K., Masuchi Y., Shizuya H., Kikuchi H.;
RT "Complete sequence and gene organization of the genome of a hyper-
RT thermophilic archaebacterium, Pyrococcus horikoshii OT3.";
RL DNA Res. 5:55-76(1998).
RN [2]
RP X-RAY CRYSTALLOGRAPHY (2.85 ANGSTROMS), AND SUBUNIT.
RA Petit P., Brown G., Savchenko A., Yakunin A.F.;
RT "Structure determination and overall comparison of three Cas1 proteins.";
RL Submitted (DEC-2010) to the PDB data bank.
CC -!- FUNCTION: CRISPR (clustered regularly interspaced short palindromic
CC repeat), is an adaptive immune system that provides protection against
CC mobile genetic elements (viruses, transposable elements and conjugative
CC plasmids). CRISPR clusters contain spacers, sequences complementary to
CC antecedent mobile elements, and target invading nucleic acids. CRISPR
CC clusters are transcribed and processed into CRISPR RNA (crRNA). Acts as
CC a dsDNA endonuclease. Involved in the integration of spacer DNA into
CC the CRISPR cassette. {ECO:0000255|HAMAP-Rule:MF_01470}.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01470};
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01470};
CC -!- SUBUNIT: Homodimer, forms a heterotetramer with a Cas2 homodimer.
CC {ECO:0000255|HAMAP-Rule:MF_01470}.
CC -!- SIMILARITY: Belongs to the CRISPR-associated endonuclease Cas1 family.
CC {ECO:0000255|HAMAP-Rule:MF_01470}.
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DR EMBL; BA000001; BAA30345.1; -; Genomic_DNA.
DR PIR; G71068; G71068.
DR RefSeq; WP_010885333.1; NC_000961.1.
DR PDB; 4WJ0; X-ray; 2.85 A; A/B=1-322.
DR PDBsum; 4WJ0; -.
DR AlphaFoldDB; O58938; -.
DR SMR; O58938; -.
DR STRING; 70601.3257662; -.
DR EnsemblBacteria; BAA30345; BAA30345; BAA30345.
DR GeneID; 1443568; -.
DR KEGG; pho:PH1245; -.
DR eggNOG; arCOG01452; Archaea.
DR OMA; AYKLIKH; -.
DR OrthoDB; 70037at2157; -.
DR EvolutionaryTrace; O58938; -.
DR Proteomes; UP000000752; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0004520; F:endodeoxyribonuclease activity; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-UniRule.
DR GO; GO:0043571; P:maintenance of CRISPR repeat elements; IEA:UniProtKB-UniRule.
DR CDD; cd09722; Cas1_I-B; 1.
DR Gene3D; 1.20.120.920; -; 1.
DR Gene3D; 3.100.10.20; -; 1.
DR HAMAP; MF_01470; Cas1; 1.
DR InterPro; IPR002729; CRISPR-assoc_Cas1.
DR InterPro; IPR042206; CRISPR-assoc_Cas1_C.
DR InterPro; IPR019858; CRISPR-assoc_Cas1_HMARI/TNEAP.
DR InterPro; IPR042211; CRISPR-assoc_Cas1_N.
DR PANTHER; PTHR43219; PTHR43219; 1.
DR Pfam; PF01867; Cas_Cas1; 1.
DR TIGRFAMs; TIGR00287; cas1; 1.
DR TIGRFAMs; TIGR03641; cas1_HMARI; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Antiviral defense; DNA-binding; Endonuclease; Hydrolase;
KW Magnesium; Manganese; Metal-binding; Nuclease.
FT CHAIN 1..322
FT /note="CRISPR-associated endonuclease Cas1"
FT /id="PRO_0000417109"
FT BINDING 149
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01470"
FT BINDING 214
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01470"
FT BINDING 229
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01470"
FT STRAND 4..8
FT /evidence="ECO:0007829|PDB:4WJ0"
FT STRAND 12..17
FT /evidence="ECO:0007829|PDB:4WJ0"
FT STRAND 20..27
FT /evidence="ECO:0007829|PDB:4WJ0"
FT STRAND 30..32
FT /evidence="ECO:0007829|PDB:4WJ0"
FT STRAND 37..42
FT /evidence="ECO:0007829|PDB:4WJ0"
FT STRAND 46..48
FT /evidence="ECO:0007829|PDB:4WJ0"
FT HELIX 50..58
FT /evidence="ECO:0007829|PDB:4WJ0"
FT STRAND 62..66
FT /evidence="ECO:0007829|PDB:4WJ0"
FT STRAND 72..78
FT /evidence="ECO:0007829|PDB:4WJ0"
FT HELIX 85..96
FT /evidence="ECO:0007829|PDB:4WJ0"
FT HELIX 98..122
FT /evidence="ECO:0007829|PDB:4WJ0"
FT HELIX 129..132
FT /evidence="ECO:0007829|PDB:4WJ0"
FT HELIX 133..138
FT /evidence="ECO:0007829|PDB:4WJ0"
FT HELIX 142..160
FT /evidence="ECO:0007829|PDB:4WJ0"
FT HELIX 161..163
FT /evidence="ECO:0007829|PDB:4WJ0"
FT HELIX 166..168
FT /evidence="ECO:0007829|PDB:4WJ0"
FT HELIX 181..202
FT /evidence="ECO:0007829|PDB:4WJ0"
FT STRAND 204..206
FT /evidence="ECO:0007829|PDB:4WJ0"
FT STRAND 212..214
FT /evidence="ECO:0007829|PDB:4WJ0"
FT HELIX 222..234
FT /evidence="ECO:0007829|PDB:4WJ0"
FT HELIX 236..245
FT /evidence="ECO:0007829|PDB:4WJ0"
FT HELIX 251..253
FT /evidence="ECO:0007829|PDB:4WJ0"
FT STRAND 254..256
FT /evidence="ECO:0007829|PDB:4WJ0"
FT STRAND 261..263
FT /evidence="ECO:0007829|PDB:4WJ0"
FT HELIX 265..279
FT /evidence="ECO:0007829|PDB:4WJ0"
FT TURN 286..288
FT /evidence="ECO:0007829|PDB:4WJ0"
FT HELIX 294..309
FT /evidence="ECO:0007829|PDB:4WJ0"
SQ SEQUENCE 322 AA; 37755 MW; F9FE7ED7D36D55B4 CRC64;
MRKKPLTIFS DGTLTRRENT LYFESAKGRK PLAIEGIYDI YIYGHVNITS QALHYIAQKG
ILIHFFNHYG YYDGTFYPRE TLLSGDLIIR QAEHYLNKEK RLFLAKSFVT GGTKNMERNL
KNWGIKAKLS DYLDELNDAR KITEIMNVEA RIRQEYYAKW DENLPEEFKI VKRTRRPPKN
EMNALISFLN SRLYATIITE IYNTQLAPTI SYLHEPSERR FSLSLDLSEI FKPIIADRVA
NRLVKKGSLK KEHFREDLNG VLLTEEGMKI VTKAYNEELQ KSVKHPKIGS NVTRQRLIRL
EAYKLIKHLV GVEEYKPLVA WF
