ID   Y457_METJA              Reviewed;         410 AA.
AC   Q57899;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 112.
DE   RecName: Full=Uncharacterized metallohydrolase MJ0457;
DE            EC=3.-.-.-;
GN   OrderedLocusNames=MJ0457;
OS   Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM
OS   10045 / NBRC 100440) (Methanococcus jannaschii).
OC   Archaea; Euryarchaeota; Methanomada group; Methanococci; Methanococcales;
OC   Methanocaldococcaceae; Methanocaldococcus.
OX   NCBI_TaxID=243232;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440;
RX   PubMed=8688087; DOI=10.1126/science.273.5278.1058;
RA   Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G.,
RA   Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R.,
RA   Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R.,
RA   Kirkness E.F., Weinstock K.G., Merrick J.M., Glodek A., Scott J.L.,
RA   Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R.,
RA   Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D.,
RA   Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P.,
RA   Fraser C.M., Smith H.O., Woese C.R., Venter J.C.;
RT   "Complete genome sequence of the methanogenic archaeon, Methanococcus
RT   jannaschii.";
RL   Science 273:1058-1073(1996).
CC   -!- COFACTOR:
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000250};
CC       Name=Co(2+); Xref=ChEBI:CHEBI:48828; Evidence={ECO:0000250};
CC       Note=Binds 2 Zn(2+) or Co(2+) ions per subunit. {ECO:0000250};
CC   -!- SIMILARITY: Belongs to the peptidase M20A family. {ECO:0000305}.
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DR   EMBL; L77117; AAB98445.1; -; Genomic_DNA.
DR   PIR; A64357; A64357.
DR   RefSeq; WP_010869956.1; NC_000909.1.
DR   AlphaFoldDB; Q57899; -.
DR   SMR; Q57899; -.
DR   STRING; 243232.MJ_0457; -.
DR   EnsemblBacteria; AAB98445; AAB98445; MJ_0457.
DR   GeneID; 1451318; -.
DR   KEGG; mja:MJ_0457; -.
DR   eggNOG; arCOG01107; Archaea.
DR   HOGENOM; CLU_021802_2_2_2; -.
DR   InParanoid; Q57899; -.
DR   OMA; STFEPTM; -.
DR   OrthoDB; 14646at2157; -.
DR   PhylomeDB; Q57899; -.
DR   Proteomes; UP000000805; Chromosome.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   InterPro; IPR010182; ArgE/DapE.
DR   InterPro; IPR036264; Bact_exopeptidase_dim_dom.
DR   InterPro; IPR002933; Peptidase_M20.
DR   InterPro; IPR011650; Peptidase_M20_dimer.
DR   Pfam; PF07687; M20_dimer; 1.
DR   Pfam; PF01546; Peptidase_M20; 1.
DR   SUPFAM; SSF55031; SSF55031; 1.
DR   TIGRFAMs; TIGR01910; DapE-ArgE; 1.
PE   3: Inferred from homology;
KW   Cobalt; Hydrolase; Metal-binding; Reference proteome; Zinc.
FT   CHAIN           1..410
FT                   /note="Uncharacterized metallohydrolase MJ0457"
FT                   /id="PRO_0000185352"
FT   ACT_SITE        89
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        154
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
FT   BINDING         87
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250"
FT   BINDING         120
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250"
FT   BINDING         120
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250"
FT   BINDING         155
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250"
FT   BINDING         184
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000250"
FT   BINDING         387
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   410 AA;  47170 MW;  90E7ADB625339D86 CRC64;
     MDLIEEAIKL ESDLIRINSV NPSFGGKGEK EKAEYVKKKL MEYVESYNIE NYTLKEYNII
     DKYGIERPNI VFKIDFGRDK TLHIISHLDT VPEGDISLWG TNPYEPVIKD GKIYGRGSED
     NHKGIVSSLL LLKMIFENNI EPKYNLSLIF VSDEEDGSEY GLKYLLNNFE DEIFKKDDLI
     IVPDFGTPTG EFVEIGEKGI LWIKFNIKGK QCHGSTPENG LNADIVAFNF ANELYNGLYE
     KFDEINSIFL PEYSTFEPTI LKNKVENPNT IPGYVEVVFD CRILPTYKIE EVLEFINKFI
     KNFEFKKYIK HYDNSIKAEI TYEILKSENP NYTDENAEII KELKKAIKNV LNRDAKLCGM
     GGGTVAAFLR YKGYNVAVWG IGEETAHQPN EHIKIEDLVK MAEVFYEILK