ID   CAS1_RICCO              Reviewed;         759 AA.
AC   Q2XPU6;
DT   16-NOV-2011, integrated into UniProtKB/Swiss-Prot.
DT   20-DEC-2005, sequence version 1.
DT   25-MAY-2022, entry version 58.
DE   RecName: Full=Cycloartenol synthase;
DE            Short=RcCAS;
DE            EC=5.4.99.8;
OS   Ricinus communis (Castor bean).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Malpighiales; Euphorbiaceae; Acalyphoideae; Acalypheae;
OC   Ricinus.
OX   NCBI_TaxID=3988;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, DEVELOPMENTAL
RP   STAGE, AND TISSUE SPECIFICITY.
RX   PubMed=16445885; DOI=10.1016/j.abb.2005.12.013;
RA   Guhling O., Hobl B., Yeats T., Jetter R.;
RT   "Cloning and characterization of a lupeol synthase involved in the
RT   synthesis of epicuticular wax crystals on stem and hypocotyl surfaces of
RT   Ricinus communis.";
RL   Arch. Biochem. Biophys. 448:60-72(2006).
CC   -!- FUNCTION: Oxidosqualene cyclase involved in the biosynthesis of
CC       cycloartenol. {ECO:0000269|PubMed:16445885}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(S)-2,3-epoxysqualene = cycloartenol; Xref=Rhea:RHEA:21308,
CC         ChEBI:CHEBI:15441, ChEBI:CHEBI:17030; EC=5.4.99.8;
CC         Evidence={ECO:0000269|PubMed:16445885};
CC   -!- TISSUE SPECIFICITY: Ubiquitous. {ECO:0000269|PubMed:16445885}.
CC   -!- DEVELOPMENTAL STAGE: Expressed constitutively.
CC       {ECO:0000269|PubMed:16445885}.
CC   -!- SIMILARITY: Belongs to the terpene cyclase/mutase family.
CC       {ECO:0000305}.
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DR   EMBL; DQ268870; ABB76767.1; -; mRNA.
DR   RefSeq; NP_001310632.1; NM_001323703.1.
DR   AlphaFoldDB; Q2XPU6; -.
DR   SMR; Q2XPU6; -.
DR   STRING; 3988.XP_002527812.1; -.
DR   GeneID; 8262543; -.
DR   KEGG; rcu:8262543; -.
DR   eggNOG; KOG0497; Eukaryota.
DR   OrthoDB; 365003at2759; -.
DR   BRENDA; 5.4.99.8; 1204.
DR   GO; GO:0005811; C:lipid droplet; IEA:InterPro.
DR   GO; GO:0016871; F:cycloartenol synthase activity; IDA:UniProtKB.
DR   GO; GO:0010686; P:tetracyclic triterpenoid biosynthetic process; IDA:UniProtKB.
DR   CDD; cd02892; SQCY_1; 1.
DR   InterPro; IPR032696; SQ_cyclase_C.
DR   InterPro; IPR032697; SQ_cyclase_N.
DR   InterPro; IPR018333; Squalene_cyclase.
DR   InterPro; IPR002365; Terpene_synthase_CS.
DR   InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR   PANTHER; PTHR11764; PTHR11764; 1.
DR   Pfam; PF13243; SQHop_cyclase_C; 1.
DR   Pfam; PF13249; SQHop_cyclase_N; 1.
DR   SFLD; SFLDG01016; Prenyltransferase_Like_2; 1.
DR   SUPFAM; SSF48239; SSF48239; 2.
DR   TIGRFAMs; TIGR01787; squalene_cyclas; 1.
DR   PROSITE; PS01074; TERPENE_SYNTHASES; 1.
PE   1: Evidence at protein level;
KW   Isomerase; Repeat.
FT   CHAIN           1..759
FT                   /note="Cycloartenol synthase"
FT                   /id="PRO_0000413993"
FT   REPEAT          149..190
FT                   /note="PFTB 1"
FT   REPEAT          514..559
FT                   /note="PFTB 2"
FT   REPEAT          591..631
FT                   /note="PFTB 3"
FT   REPEAT          640..681
FT                   /note="PFTB 4"
FT   REPEAT          702..743
FT                   /note="PFTB 5"
FT   ACT_SITE        485
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250|UniProtKB:P48449"
SQ   SEQUENCE   759 AA;  86590 MW;  7F1DCA5CF124A71C CRC64;
     MWKLRIAEGS GNPWLRTTND HIGRQVWEFD SSKIGSPEEL SQIENARQNF TKNRFIHKHS
     SDLLMRIQFS KENPICEVLP QVKVKESEQV TEEKVKITLR RALNYYSSIQ ADDGHWPGDY
     GGPMFLMPGL IIALSITGAL NAILSEEHKR EMCRYLYNHQ NRDGGWGLHI EGPSTMFGSV
     LCYVSLRLLG EGPNEGEGAV ERGRNWILKH GGATAITSWG KMWLSVLGAY EWSGNNPLPP
     EMWLLPYILP VHPGRMWCHC RMVYLPMSYL YGKRFVGPIT PTVLSLRKEL YTVPYHEIDW
     NQARNQCAKE DLYYPHPMLQ DVLWATLHKF VEPILMHWPG KRLREKAIQT AIEHIHYEDE
     NTRYICIGPV NKVLNMLCCW VEDPNSEAFK LHLPRLYDYL WLAEDGMKMQ GYNGSQLWDT
     AFAVQAIVST NLIEEYGPTL KKAHSFIKKM QVLENCPGDL NFWYRHISKG AWPFSTADHG
     WPISDCTAEG IKALMLLSKI PSEIVGEGLN ANRLYDAVNV VLSLQNGDGG FPTYELSRSY
     SWLEFINPAE TFGDIVIDYP YVECTSAAIQ ALTSFRKSYP EHQREEIECC IKKAAKFMEK
     IQISDGSWYG SWGVCFTYGT WFGIKGLVAA GKSFGNCSSI RKACDFLLSK QCPSGGWGES
     YLSCQKKVYS NLEGDRSHVV NTAWAMLSLI DAGQAERDPT PLHRAARYLI NAQMENGDFP
     QQEIMGVFNR NCMITYAAYR DIFPIWALGE YRCRVLKAS