ID   CAS1_THEKO              Reviewed;         326 AA.
AC   Q5JGA9;
DT   16-MAY-2012, integrated into UniProtKB/Swiss-Prot.
DT   15-FEB-2005, sequence version 1.
DT   03-AUG-2022, entry version 92.
DE   RecName: Full=CRISPR-associated endonuclease Cas1 {ECO:0000255|HAMAP-Rule:MF_01470};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_01470};
GN   Name=cas1 {ECO:0000255|HAMAP-Rule:MF_01470}; OrderedLocusNames=TK0455;
OS   Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1)
OS   (Pyrococcus kodakaraensis (strain KOD1)).
OC   Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC   Thermococcus.
OX   NCBI_TaxID=69014;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-918 / JCM 12380 / KOD1;
RX   PubMed=15710748; DOI=10.1101/gr.3003105;
RA   Fukui T., Atomi H., Kanai T., Matsumi R., Fujiwara S., Imanaka T.;
RT   "Complete genome sequence of the hyperthermophilic archaeon Thermococcus
RT   kodakaraensis KOD1 and comparison with Pyrococcus genomes.";
RL   Genome Res. 15:352-363(2005).
CC   -!- FUNCTION: CRISPR (clustered regularly interspaced short palindromic
CC       repeat), is an adaptive immune system that provides protection against
CC       mobile genetic elements (viruses, transposable elements and conjugative
CC       plasmids). CRISPR clusters contain spacers, sequences complementary to
CC       antecedent mobile elements, and target invading nucleic acids. CRISPR
CC       clusters are transcribed and processed into CRISPR RNA (crRNA). Acts as
CC       a dsDNA endonuclease. Involved in the integration of spacer DNA into
CC       the CRISPR cassette. {ECO:0000255|HAMAP-Rule:MF_01470}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01470};
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01470};
CC   -!- SUBUNIT: Homodimer, forms a heterotetramer with a Cas2 homodimer.
CC       {ECO:0000255|HAMAP-Rule:MF_01470}.
CC   -!- SIMILARITY: Belongs to the CRISPR-associated endonuclease Cas1 family.
CC       {ECO:0000255|HAMAP-Rule:MF_01470}.
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DR   EMBL; AP006878; BAD84644.1; -; Genomic_DNA.
DR   RefSeq; WP_011249410.1; NC_006624.1.
DR   AlphaFoldDB; Q5JGA9; -.
DR   SMR; Q5JGA9; -.
DR   STRING; 69014.TK0455; -.
DR   EnsemblBacteria; BAD84644; BAD84644; TK0455.
DR   GeneID; 3234082; -.
DR   KEGG; tko:TK0455; -.
DR   PATRIC; fig|69014.16.peg.448; -.
DR   eggNOG; arCOG01452; Archaea.
DR   HOGENOM; CLU_052779_2_0_2; -.
DR   InParanoid; Q5JGA9; -.
DR   OMA; AYKLIKH; -.
DR   OrthoDB; 70037at2157; -.
DR   PhylomeDB; Q5JGA9; -.
DR   Proteomes; UP000000536; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0004520; F:endodeoxyribonuclease activity; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-UniRule.
DR   GO; GO:0043571; P:maintenance of CRISPR repeat elements; IEA:UniProtKB-UniRule.
DR   CDD; cd09722; Cas1_I-B; 1.
DR   Gene3D; 1.20.120.920; -; 1.
DR   Gene3D; 3.100.10.20; -; 1.
DR   HAMAP; MF_01470; Cas1; 1.
DR   InterPro; IPR002729; CRISPR-assoc_Cas1.
DR   InterPro; IPR042206; CRISPR-assoc_Cas1_C.
DR   InterPro; IPR019858; CRISPR-assoc_Cas1_HMARI/TNEAP.
DR   InterPro; IPR042211; CRISPR-assoc_Cas1_N.
DR   PANTHER; PTHR43219; PTHR43219; 1.
DR   Pfam; PF01867; Cas_Cas1; 1.
DR   TIGRFAMs; TIGR00287; cas1; 1.
DR   TIGRFAMs; TIGR03641; cas1_HMARI; 1.
PE   3: Inferred from homology;
KW   Antiviral defense; DNA-binding; Endonuclease; Hydrolase; Magnesium;
KW   Manganese; Metal-binding; Nuclease; Reference proteome.
FT   CHAIN           1..326
FT                   /note="CRISPR-associated endonuclease Cas1"
FT                   /id="PRO_0000417110"
FT   BINDING         153
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01470"
FT   BINDING         218
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01470"
FT   BINDING         233
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01470"
SQ   SEQUENCE   326 AA;  37858 MW;  B43BBCCA5BDBFD68 CRC64;
     MRKRSLTLFS DGNLFRRENT LYFENAQGRK PLAVEGIYDI YVYGHVNISS QALHFLAQKG
     IPVHFFNHYG HYDGSFYPRE SLLSGDLIIK QAKHYLDQEK RLILAKLFVR GSALNMEKNL
     KRWKVADGFS GLLDELFEEL ESARKITEVM NVEARIRGEY YTRWDNALPE GFKIVKRTRR
     PPENEMNALI SFLNSRLYAT IVSELYNTQL APTVSYLHEP GERRFSLALD LSEIFKPIIA
     DRIANRLVKQ GIIKREHFRG ELNGVLLTKE GMKVVLKAYN EELGRSVKHP GLKKNVTKQR
     LIRLEAYKLI KHLVGVKDYE PLVAWF