ID   Y4618_METAC             Reviewed;         742 AA.
AC   Q8THA2;
DT   24-MAY-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   03-AUG-2022, entry version 95.
DE   RecName: Full=UPF0313 protein MA_4618;
GN   OrderedLocusNames=MA_4618;
OS   Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 /
OS   C2A).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC   Methanosarcinales; Methanosarcinaceae; Methanosarcina.
OX   NCBI_TaxID=188937;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 35395 / DSM 2834 / JCM 12185 / C2A;
RX   PubMed=11932238; DOI=10.1101/gr.223902;
RA   Galagan J.E., Nusbaum C., Roy A., Endrizzi M.G., Macdonald P., FitzHugh W.,
RA   Calvo S., Engels R., Smirnov S., Atnoor D., Brown A., Allen N., Naylor J.,
RA   Stange-Thomann N., DeArellano K., Johnson R., Linton L., McEwan P.,
RA   McKernan K., Talamas J., Tirrell A., Ye W., Zimmer A., Barber R.D.,
RA   Cann I., Graham D.E., Grahame D.A., Guss A.M., Hedderich R.,
RA   Ingram-Smith C., Kuettner H.C., Krzycki J.A., Leigh J.A., Li W., Liu J.,
RA   Mukhopadhyay B., Reeve J.N., Smith K., Springer T.A., Umayam L.A.,
RA   White O., White R.H., de Macario E.C., Ferry J.G., Jarrell K.F., Jing H.,
RA   Macario A.J.L., Paulsen I.T., Pritchett M., Sowers K.R., Swanson R.V.,
RA   Zinder S.H., Lander E., Metcalf W.W., Birren B.;
RT   "The genome of Methanosarcina acetivorans reveals extensive metabolic and
RT   physiological diversity.";
RL   Genome Res. 12:532-542(2002).
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Evidence={ECO:0000305};
CC       Note=Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3
CC       cysteines and an exchangeable S-adenosyl-L-methionine. {ECO:0000305};
CC   -!- SIMILARITY: In the C-terminal section; belongs to the UPF0313 family.
CC       {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AE010299; AAM07954.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q8THA2; -.
DR   STRING; 188937.MA_4618; -.
DR   EnsemblBacteria; AAM07954; AAM07954; MA_4618.
DR   KEGG; mac:MA_4618; -.
DR   HOGENOM; CLU_018288_2_0_2; -.
DR   InParanoid; Q8THA2; -.
DR   OMA; TCMYYTG; -.
DR   PhylomeDB; Q8THA2; -.
DR   Proteomes; UP000002487; Chromosome.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.80.30.20; -; 1.
DR   HAMAP; MF_01251; UPF0313; 1.
DR   InterPro; IPR006638; Elp3/MiaB/NifB.
DR   InterPro; IPR020612; Methylthiotransferase_CS.
DR   InterPro; IPR007197; rSAM.
DR   InterPro; IPR023404; rSAM_horseshoe.
DR   InterPro; IPR022946; UPF0313.
DR   InterPro; IPR024560; UPF0313_C.
DR   InterPro; IPR013704; UPF0313_N.
DR   PANTHER; PTHR32331; PTHR32331; 1.
DR   Pfam; PF11842; DUF3362; 1.
DR   Pfam; PF04055; Radical_SAM; 1.
DR   Pfam; PF08497; Radical_SAM_N; 1.
DR   SFLD; SFLDS00029; Radical_SAM; 1.
DR   SFLD; SFLDG01069; UPF0313; 1.
DR   SMART; SM00729; Elp3; 1.
DR   TIGRFAMs; TIGR03904; SAM_YgiQ; 1.
DR   PROSITE; PS51918; RADICAL_SAM; 1.
PE   3: Inferred from homology;
KW   4Fe-4S; Iron; Iron-sulfur; Metal-binding; Reference proteome;
KW   S-adenosyl-L-methionine.
FT   CHAIN           1..742
FT                   /note="UPF0313 protein MA_4618"
FT                   /id="PRO_0000076403"
FT   DOMAIN          438..707
FT                   /note="Radical SAM core"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01266"
FT   REGION          1..128
FT                   /note="Unknown"
FT   REGION          1..125
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          129..742
FT                   /note="UPF0313"
FT   COMPBIAS        16..73
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        89..107
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         452
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT                   /evidence="ECO:0000255"
FT   BINDING         456
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT                   /evidence="ECO:0000255"
FT   BINDING         459
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   742 AA;  83276 MW;  82BDB029802A937F CRC64;
     MGVRKQTMVK DTAVPIENKK FTREKKDKEK DKKPDRERAG RKTGFFVPKK VETGKKGKLG
     RKSEGKAEEK AEGKAGISTS TISERAVLKP SAGKRKKAES GPGTKTGKKE KKQKKSFSSS
     LPASKFLPMS PEEVKARGWK ELDIILVTGD AYVDHSSFGT AIIGRVLEDA GFRVGIIAQP
     RWDNPEDLKK LGRPRLFFSV SAGNTDSMVS NLTPGLKPRK KDAYSPGNKT GLRPNRSVII
     YSNRIKEAFP NVPIVLGGIE ASLRRFAHYD YLSDKVRQAI LADAPADLVV YGMGELQIVE
     IAKRLQAGED IRKIRDIPGT VWKMEVRAWK ELKEKGKGTN EAVARDTAPE AAEFFKEYIE
     IPSFSEVSQD KAAFAKAFRT YFLEQNPITG KGIVQPHPKT VIVQNRPMRP LTEAELDHVY
     ELPYTGEAHP SYTEPIPALE MVKFSLTTHR GCFGGCSFCA ITQHQGRMIT SRSIESVLRE
     AKKLTEKPDF KGIINGVGGP TANMYGMGCR SWEKQGACLD KACLYPRVCP ALDTSHKKLL
     ELMKRLRELP GVKHVFTGYG VRYDLALEDE EYLEELCTHH ISGQLRIAPE HFSKRVTDAM
     SKPGKKVYER FSEKFAAFNK KCGKEQYIVN YLMSGHPGCT LKDMIEMAEY LRDHGGYTEQ
     VQDFTPTPMT VSTCMYYTGL DPFTGKKVYV AKDKKEKAMQ RALMHYRSPA NYELVYEALE
     KAGRLDLVGN AHKCLIRRKE KQ