ID   CAS2A_ARCFU             Reviewed;          94 AA.
AC   O28403;
DT   18-APR-2012, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   03-AUG-2022, entry version 89.
DE   RecName: Full=CRISPR-associated endoribonuclease Cas2 1;
DE            EC=3.1.-.-;
GN   Name=cas21; OrderedLocusNames=AF_1876;
OS   Archaeoglobus fulgidus (strain ATCC 49558 / DSM 4304 / JCM 9628 / NBRC
OS   100126 / VC-16).
OC   Archaea; Euryarchaeota; Archaeoglobi; Archaeoglobales; Archaeoglobaceae;
OC   Archaeoglobus.
OX   NCBI_TaxID=224325;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 49558 / DSM 4304 / JCM 9628 / NBRC 100126 / VC-16;
RX   PubMed=9389475; DOI=10.1038/37052;
RA   Klenk H.-P., Clayton R.A., Tomb J.-F., White O., Nelson K.E., Ketchum K.A.,
RA   Dodson R.J., Gwinn M.L., Hickey E.K., Peterson J.D., Richardson D.L.,
RA   Kerlavage A.R., Graham D.E., Kyrpides N.C., Fleischmann R.D.,
RA   Quackenbush J., Lee N.H., Sutton G.G., Gill S.R., Kirkness E.F.,
RA   Dougherty B.A., McKenney K., Adams M.D., Loftus B.J., Peterson S.N.,
RA   Reich C.I., McNeil L.K., Badger J.H., Glodek A., Zhou L., Overbeek R.,
RA   Gocayne J.D., Weidman J.F., McDonald L.A., Utterback T.R., Cotton M.D.,
RA   Spriggs T., Artiach P., Kaine B.P., Sykes S.M., Sadow P.W., D'Andrea K.P.,
RA   Bowman C., Fujii C., Garland S.A., Mason T.M., Olsen G.J., Fraser C.M.,
RA   Smith H.O., Woese C.R., Venter J.C.;
RT   "The complete genome sequence of the hyperthermophilic, sulphate-reducing
RT   archaeon Archaeoglobus fulgidus.";
RL   Nature 390:364-370(1997).
RN   [2]
RP   FUNCTION AS A SSRNA-SPECIFIC ENDORIBONUCLEASE.
RC   STRAIN=ATCC 49558 / DSM 4304 / JCM 9628 / NBRC 100126 / VC-16;
RX   PubMed=18482976; DOI=10.1074/jbc.m803225200;
RA   Beloglazova N., Brown G., Zimmerman M.D., Proudfoot M., Makarova K.S.,
RA   Kudritska M., Kochinyan S., Wang S., Chruszcz M., Minor W., Koonin E.V.,
RA   Edwards A.M., Savchenko A., Yakunin A.F.;
RT   "A novel family of sequence-specific endoribonucleases associated with the
RT   clustered regularly interspaced short palindromic repeats.";
RL   J. Biol. Chem. 283:20361-20371(2008).
CC   -!- FUNCTION: CRISPR (clustered regularly interspaced short palindromic
CC       repeat), is an adaptive immune system that provides protection against
CC       mobile genetic elements (viruses, transposable elements and conjugative
CC       plasmids). CRISPR clusters contain sequences complementary to
CC       antecedent mobile elements and target invading nucleic acids. CRISPR
CC       clusters are transcribed and processed into CRISPR RNA (crRNA).
CC       Involved in the integration of spacer DNA into the CRISPR cassette (By
CC       similarity). Functions as a ssRNA-specific endoribonuclease.
CC       {ECO:0000250, ECO:0000269|PubMed:18482976}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC   -!- SUBUNIT: Homodimer, forms a heterotetramer with a Cas1 homodimer.
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the CRISPR-associated endoribonuclease Cas2
CC       protein family. {ECO:0000305}.
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DR   EMBL; AE000782; AAB89376.1; -; Genomic_DNA.
DR   PIR; C69484; C69484.
DR   RefSeq; WP_010879369.1; NC_000917.1.
DR   AlphaFoldDB; O28403; -.
DR   SMR; O28403; -.
DR   STRING; 224325.AF_1876; -.
DR   DNASU; 1485097; -.
DR   EnsemblBacteria; AAB89376; AAB89376; AF_1876.
DR   GeneID; 24795621; -.
DR   KEGG; afu:AF_1876; -.
DR   eggNOG; arCOG04194; Archaea.
DR   HOGENOM; CLU_161124_2_0_2; -.
DR   OMA; IDIFPIC; -.
DR   OrthoDB; 123741at2157; -.
DR   PhylomeDB; O28403; -.
DR   Proteomes; UP000002199; Chromosome.
DR   GO; GO:0004521; F:endoribonuclease activity; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-UniRule.
DR   GO; GO:0043571; P:maintenance of CRISPR repeat elements; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_01471; Cas2; 1.
DR   InterPro; IPR021127; CRISPR_associated_Cas2.
DR   InterPro; IPR019199; Virulence_VapD/CRISPR_Cas2.
DR   PANTHER; PTHR34405; PTHR34405; 1.
DR   Pfam; PF09827; CRISPR_Cas2; 1.
DR   TIGRFAMs; TIGR01573; cas2; 1.
PE   1: Evidence at protein level;
KW   Antiviral defense; Endonuclease; Hydrolase; Magnesium; Metal-binding;
KW   Nuclease; Reference proteome.
FT   CHAIN           1..94
FT                   /note="CRISPR-associated endoribonuclease Cas2 1"
FT                   /id="PRO_0000416948"
FT   BINDING         8
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   94 AA;  10657 MW;  FDD6FB00B313918E CRC64;
     MLHLVVYDIS DDGSRARLAK LLEKFGLQRV QYSAFRGELN PNDREVLARQ VGKFVRDDRD
     CIFIIPLCQR CSSTAIVISN TGVELVKEKG VEFV