ID   CAS2A_FRATN             Reviewed;          97 AA.
AC   A0Q5Y5;
DT   11-MAY-2016, integrated into UniProtKB/Swiss-Prot.
DT   09-JAN-2007, sequence version 1.
DT   03-AUG-2022, entry version 78.
DE   RecName: Full=CRISPR-associated endoribonuclease Cas2 1 {ECO:0000255|HAMAP-Rule:MF_01471};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_01471};
GN   Name=cas2-1 {ECO:0000255|HAMAP-Rule:MF_01471};
GN   OrderedLocusNames=FTN_0759 {ECO:0000312|EMBL:ABK89650.1};
OS   Francisella tularensis subsp. novicida (strain U112).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Thiotrichales;
OC   Francisellaceae; Francisella.
OX   NCBI_TaxID=401614;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=U112;
RX   PubMed=17550600; DOI=10.1186/gb-2007-8-6-r102;
RA   Rohmer L., Fong C., Abmayr S., Wasnick M., Larson Freeman T.J., Radey M.,
RA   Guina T., Svensson K., Hayden H.S., Jacobs M., Gallagher L.A., Manoil C.,
RA   Ernst R.K., Drees B., Buckley D., Haugen E., Bovee D., Zhou Y., Chang J.,
RA   Levy R., Lim R., Gillett W., Guenthener D., Kang A., Shaffer S.A.,
RA   Taylor G., Chen J., Gallis B., D'Argenio D.A., Forsman M., Olson M.V.,
RA   Goodlett D.R., Kaul R., Miller S.I., Brittnacher M.J.;
RT   "Comparison of Francisella tularensis genomes reveals evolutionary events
RT   associated with the emergence of human pathogenic strains.";
RL   Genome Biol. 8:R102.1-R102.16(2007).
RN   [2]
RP   INDUCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=U112;
RX   PubMed=23584588; DOI=10.1038/nature12048;
RA   Sampson T.R., Saroj S.D., Llewellyn A.C., Tzeng Y.L., Weiss D.S.;
RT   "A CRISPR/Cas system mediates bacterial innate immune evasion and
RT   virulence.";
RL   Nature 497:254-257(2013).
RN   [3]
RP   ERRATUM OF PUBMED:23584588.
RX   DOI=10.1038/nature12498;
RA   Sampson T.R., Saroj S.D., Llewellyn A.C., Tzeng Y.L., Weiss D.S.;
RL   Nature 501:602-602(2013).
CC   -!- FUNCTION: CRISPR (clustered regularly interspaced short palindromic
CC       repeat) is an adaptive immune system that provides protection against
CC       mobile genetic elements (viruses, transposable elements and conjugative
CC       plasmids). CRISPR clusters contain sequences complementary to
CC       antecedent mobile elements and target invading nucleic acids. CRISPR
CC       clusters are transcribed and processed into CRISPR RNA (crRNA).
CC       Functions as a ssRNA-specific endoribonuclease. Involved in the
CC       integration of spacer DNA into the CRISPR cassette. {ECO:0000255|HAMAP-
CC       Rule:MF_01471}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01471};
CC   -!- SUBUNIT: Homodimer, forms a heterotetramer with a Cas1 homodimer.
CC       {ECO:0000255|HAMAP-Rule:MF_01471}.
CC   -!- INDUCTION: In culture expression is high during mid-log phase (2 hour),
CC       then decreases to nearly undetectable levels in late stationary phase
CC       (16 hours). During infection of mouse bone marrow-derived macrophages
CC       (BMDM) expression is maximal after 1 hour, when the bacteria is
CC       expected to be in the phagosome. {ECO:0000269|PubMed:23584588}.
CC   -!- DISRUPTION PHENOTYPE: No effect on expression of bacterial lipoprotein
CC       FTN_1103. Bacteria are as virulent in mice as wild-type bacteria.
CC       {ECO:0000269|PubMed:23584588}.
CC   -!- MISCELLANEOUS: Part of a type II CRISPR-Cas system.
CC       {ECO:0000305|PubMed:23584588}.
CC   -!- SIMILARITY: Belongs to the CRISPR-associated endoribonuclease Cas2
CC       protein family. {ECO:0000255|HAMAP-Rule:MF_01471}.
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DR   EMBL; CP000439; ABK89650.1; -; Genomic_DNA.
DR   RefSeq; WP_003026686.1; NZ_CP009633.1.
DR   AlphaFoldDB; A0Q5Y5; -.
DR   SMR; A0Q5Y5; -.
DR   EnsemblBacteria; ABK89650; ABK89650; FTN_0759.
DR   GeneID; 60807049; -.
DR   KEGG; ftn:FTN_0759; -.
DR   OMA; KSVFWGH; -.
DR   OrthoDB; 2078871at2; -.
DR   BioCyc; FTUL401614:G1G75-791-MON; -.
DR   Proteomes; UP000000762; Chromosome.
DR   GO; GO:0004521; F:endoribonuclease activity; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-UniRule.
DR   GO; GO:0043571; P:maintenance of CRISPR repeat elements; IEA:UniProtKB-UniRule.
DR   CDD; cd09725; Cas2_I_II_III; 1.
DR   HAMAP; MF_01471; Cas2; 1.
DR   InterPro; IPR021127; CRISPR_associated_Cas2.
DR   InterPro; IPR019199; Virulence_VapD/CRISPR_Cas2.
DR   Pfam; PF09827; CRISPR_Cas2; 1.
DR   TIGRFAMs; TIGR01573; cas2; 1.
PE   2: Evidence at transcript level;
KW   Antiviral defense; Endonuclease; Hydrolase; Magnesium; Metal-binding;
KW   Nuclease.
FT   CHAIN           1..97
FT                   /note="CRISPR-associated endoribonuclease Cas2 1"
FT                   /id="PRO_0000436105"
FT   BINDING         12
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01471"
SQ   SEQUENCE   97 AA;  11555 MW;  524575FBD6638669 CRC64;
     MQLRKEYLIA YDIEDNKTRT IIYKQLLAYG LKAVQKSVFW GYVSIAELNA IKRLFDSSLT
     ISDKVFITRV NMHEQKLDYS FGYDDKTFKD WDEYGHI