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Y4692_MYCS2
ID   Y4692_MYCS2             Reviewed;         157 AA.
AC   A0R1B5; I7G5U1;
DT   10-AUG-2010, integrated into UniProtKB/Swiss-Prot.
DT   09-JAN-2007, sequence version 1.
DT   03-AUG-2022, entry version 75.
DE   RecName: Full=Uncharacterized protein MSMEG_4692/MSMEI_4575;
GN   OrderedLocusNames=MSMEG_4692, MSMEI_4575;
OS   Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium
OS   smegmatis).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycolicibacterium.
OX   NCBI_TaxID=246196;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RA   Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C.,
RA   Fraser C.M.;
RL   Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=17295914; DOI=10.1186/gb-2007-8-2-r20;
RA   Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C.,
RA   Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.;
RT   "Interrupted coding sequences in Mycobacterium smegmatis: authentic
RT   mutations or sequencing errors?";
RL   Genome Biol. 8:R20.1-R20.9(2007).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=18955433; DOI=10.1101/gr.081901.108;
RA   Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M.,
RA   Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.;
RT   "Ortho-proteogenomics: multiple proteomes investigation through orthology
RT   and a new MS-based protocol.";
RL   Genome Res. 19:128-135(2009).
RN   [4]
RP   PUPYLATION AT LYS-115, AND IDENTIFICATION BY MASS SPECTROMETRY.
RX   PubMed=20094657; DOI=10.1039/b916104j;
RA   Watrous J., Burns K., Liu W.T., Patel A., Hook V., Bafna V.,
RA   Barry C.E. III, Bark S., Dorrestein P.C.;
RT   "Expansion of the mycobacterial 'PUPylome'.";
RL   Mol. Biosyst. 6:376-385(2010).
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DR   EMBL; CP000480; ABK73349.1; -; Genomic_DNA.
DR   EMBL; CP001663; AFP41027.1; -; Genomic_DNA.
DR   RefSeq; WP_003896073.1; NZ_SIJM01000004.1.
DR   RefSeq; YP_888953.1; NC_008596.1.
DR   PDB; 6ADQ; EM; 3.50 A; J/V=1-157.
DR   PDB; 6HWH; EM; 3.30 A; O/T=1-157.
DR   PDB; 7E1V; EM; 2.68 A; J/V=1-157.
DR   PDB; 7E1W; EM; 2.67 A; J/V=1-157.
DR   PDB; 7E1X; EM; 2.93 A; J/V=1-157.
DR   PDB; 7RH5; EM; 3.00 A; V/b=13-157.
DR   PDB; 7RH6; EM; 3.50 A; V/b=13-157.
DR   PDB; 7RH7; EM; 3.00 A; V/b=13-157.
DR   PDBsum; 6ADQ; -.
DR   PDBsum; 6HWH; -.
DR   PDBsum; 7E1V; -.
DR   PDBsum; 7E1W; -.
DR   PDBsum; 7E1X; -.
DR   PDBsum; 7RH5; -.
DR   PDBsum; 7RH6; -.
DR   PDBsum; 7RH7; -.
DR   AlphaFoldDB; A0R1B5; -.
DR   SMR; A0R1B5; -.
DR   IntAct; A0R1B5; 2.
DR   STRING; 246196.MSMEI_4575; -.
DR   PRIDE; A0R1B5; -.
DR   EnsemblBacteria; ABK73349; ABK73349; MSMEG_4692.
DR   EnsemblBacteria; AFP41027; AFP41027; MSMEI_4575.
DR   GeneID; 66736008; -.
DR   KEGG; msg:MSMEI_4575; -.
DR   KEGG; msm:MSMEG_4692; -.
DR   PATRIC; fig|246196.19.peg.4582; -.
DR   eggNOG; COG1512; Bacteria.
DR   OMA; LSVQYPF; -.
DR   OrthoDB; 1967752at2; -.
DR   Proteomes; UP000000757; Chromosome.
DR   Proteomes; UP000006158; Chromosome.
DR   InterPro; IPR033437; DUF5130.
DR   Pfam; PF17174; DUF5130; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Isopeptide bond; Reference proteome; Ubl conjugation.
FT   CHAIN           1..157
FT                   /note="Uncharacterized protein MSMEG_4692/MSMEI_4575"
FT                   /id="PRO_0000396086"
FT   CROSSLNK        115
FT                   /note="Isoglutamyl lysine isopeptide (Lys-Gln) (interchain
FT                   with Q-Cter in protein Pup)"
FT                   /evidence="ECO:0000269|PubMed:20094657"
FT   STRAND          17..19
FT                   /evidence="ECO:0007829|PDB:7RH5"
FT   STRAND          24..26
FT                   /evidence="ECO:0007829|PDB:7E1W"
FT   STRAND          34..36
FT                   /evidence="ECO:0007829|PDB:7RH7"
FT   HELIX           45..60
FT                   /evidence="ECO:0007829|PDB:7E1W"
FT   TURN            61..63
FT                   /evidence="ECO:0007829|PDB:7E1W"
FT   STRAND          64..69
FT                   /evidence="ECO:0007829|PDB:7E1W"
FT   HELIX           77..84
FT                   /evidence="ECO:0007829|PDB:7E1W"
FT   HELIX           85..87
FT                   /evidence="ECO:0007829|PDB:7E1W"
FT   STRAND          88..90
FT                   /evidence="ECO:0007829|PDB:7E1W"
FT   STRAND          93..100
FT                   /evidence="ECO:0007829|PDB:7E1W"
FT   TURN            101..104
FT                   /evidence="ECO:0007829|PDB:7E1W"
FT   STRAND          105..109
FT                   /evidence="ECO:0007829|PDB:7E1W"
FT   STRAND          112..114
FT                   /evidence="ECO:0007829|PDB:7E1W"
FT   STRAND          116..118
FT                   /evidence="ECO:0007829|PDB:7E1W"
FT   TURN            120..122
FT                   /evidence="ECO:0007829|PDB:7E1W"
FT   HELIX           123..134
FT                   /evidence="ECO:0007829|PDB:7E1W"
FT   TURN            135..137
FT                   /evidence="ECO:0007829|PDB:7E1W"
FT   HELIX           139..153
FT                   /evidence="ECO:0007829|PDB:7E1W"
SQ   SEQUENCE   157 AA;  15899 MW;  0681183BDB1ACB66 CRC64;
     MASGDIATVA NAELDLPYGS ALTSSGRISA VTEPGELSVH YPFPTMDLVV LDDALKYGSR
     AAKARFAVYI GPLGADTAAT AREILANVPT PENAVLLAVS PDQRAIEVVY GADVKGRGIE
     SAAPLGVSAA AASFKEGNLI DGLISAVRVM SAGVSPA
 
 
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