Y4692_MYCS2
ID Y4692_MYCS2 Reviewed; 157 AA.
AC A0R1B5; I7G5U1;
DT 10-AUG-2010, integrated into UniProtKB/Swiss-Prot.
DT 09-JAN-2007, sequence version 1.
DT 03-AUG-2022, entry version 75.
DE RecName: Full=Uncharacterized protein MSMEG_4692/MSMEI_4575;
GN OrderedLocusNames=MSMEG_4692, MSMEI_4575;
OS Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium
OS smegmatis).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycolicibacterium.
OX NCBI_TaxID=246196;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RA Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C.,
RA Fraser C.M.;
RL Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=17295914; DOI=10.1186/gb-2007-8-2-r20;
RA Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C.,
RA Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.;
RT "Interrupted coding sequences in Mycobacterium smegmatis: authentic
RT mutations or sequencing errors?";
RL Genome Biol. 8:R20.1-R20.9(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=18955433; DOI=10.1101/gr.081901.108;
RA Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M.,
RA Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.;
RT "Ortho-proteogenomics: multiple proteomes investigation through orthology
RT and a new MS-based protocol.";
RL Genome Res. 19:128-135(2009).
RN [4]
RP PUPYLATION AT LYS-115, AND IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=20094657; DOI=10.1039/b916104j;
RA Watrous J., Burns K., Liu W.T., Patel A., Hook V., Bafna V.,
RA Barry C.E. III, Bark S., Dorrestein P.C.;
RT "Expansion of the mycobacterial 'PUPylome'.";
RL Mol. Biosyst. 6:376-385(2010).
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DR EMBL; CP000480; ABK73349.1; -; Genomic_DNA.
DR EMBL; CP001663; AFP41027.1; -; Genomic_DNA.
DR RefSeq; WP_003896073.1; NZ_SIJM01000004.1.
DR RefSeq; YP_888953.1; NC_008596.1.
DR PDB; 6ADQ; EM; 3.50 A; J/V=1-157.
DR PDB; 6HWH; EM; 3.30 A; O/T=1-157.
DR PDB; 7E1V; EM; 2.68 A; J/V=1-157.
DR PDB; 7E1W; EM; 2.67 A; J/V=1-157.
DR PDB; 7E1X; EM; 2.93 A; J/V=1-157.
DR PDB; 7RH5; EM; 3.00 A; V/b=13-157.
DR PDB; 7RH6; EM; 3.50 A; V/b=13-157.
DR PDB; 7RH7; EM; 3.00 A; V/b=13-157.
DR PDBsum; 6ADQ; -.
DR PDBsum; 6HWH; -.
DR PDBsum; 7E1V; -.
DR PDBsum; 7E1W; -.
DR PDBsum; 7E1X; -.
DR PDBsum; 7RH5; -.
DR PDBsum; 7RH6; -.
DR PDBsum; 7RH7; -.
DR AlphaFoldDB; A0R1B5; -.
DR SMR; A0R1B5; -.
DR IntAct; A0R1B5; 2.
DR STRING; 246196.MSMEI_4575; -.
DR PRIDE; A0R1B5; -.
DR EnsemblBacteria; ABK73349; ABK73349; MSMEG_4692.
DR EnsemblBacteria; AFP41027; AFP41027; MSMEI_4575.
DR GeneID; 66736008; -.
DR KEGG; msg:MSMEI_4575; -.
DR KEGG; msm:MSMEG_4692; -.
DR PATRIC; fig|246196.19.peg.4582; -.
DR eggNOG; COG1512; Bacteria.
DR OMA; LSVQYPF; -.
DR OrthoDB; 1967752at2; -.
DR Proteomes; UP000000757; Chromosome.
DR Proteomes; UP000006158; Chromosome.
DR InterPro; IPR033437; DUF5130.
DR Pfam; PF17174; DUF5130; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Isopeptide bond; Reference proteome; Ubl conjugation.
FT CHAIN 1..157
FT /note="Uncharacterized protein MSMEG_4692/MSMEI_4575"
FT /id="PRO_0000396086"
FT CROSSLNK 115
FT /note="Isoglutamyl lysine isopeptide (Lys-Gln) (interchain
FT with Q-Cter in protein Pup)"
FT /evidence="ECO:0000269|PubMed:20094657"
FT STRAND 17..19
FT /evidence="ECO:0007829|PDB:7RH5"
FT STRAND 24..26
FT /evidence="ECO:0007829|PDB:7E1W"
FT STRAND 34..36
FT /evidence="ECO:0007829|PDB:7RH7"
FT HELIX 45..60
FT /evidence="ECO:0007829|PDB:7E1W"
FT TURN 61..63
FT /evidence="ECO:0007829|PDB:7E1W"
FT STRAND 64..69
FT /evidence="ECO:0007829|PDB:7E1W"
FT HELIX 77..84
FT /evidence="ECO:0007829|PDB:7E1W"
FT HELIX 85..87
FT /evidence="ECO:0007829|PDB:7E1W"
FT STRAND 88..90
FT /evidence="ECO:0007829|PDB:7E1W"
FT STRAND 93..100
FT /evidence="ECO:0007829|PDB:7E1W"
FT TURN 101..104
FT /evidence="ECO:0007829|PDB:7E1W"
FT STRAND 105..109
FT /evidence="ECO:0007829|PDB:7E1W"
FT STRAND 112..114
FT /evidence="ECO:0007829|PDB:7E1W"
FT STRAND 116..118
FT /evidence="ECO:0007829|PDB:7E1W"
FT TURN 120..122
FT /evidence="ECO:0007829|PDB:7E1W"
FT HELIX 123..134
FT /evidence="ECO:0007829|PDB:7E1W"
FT TURN 135..137
FT /evidence="ECO:0007829|PDB:7E1W"
FT HELIX 139..153
FT /evidence="ECO:0007829|PDB:7E1W"
SQ SEQUENCE 157 AA; 15899 MW; 0681183BDB1ACB66 CRC64;
MASGDIATVA NAELDLPYGS ALTSSGRISA VTEPGELSVH YPFPTMDLVV LDDALKYGSR
AAKARFAVYI GPLGADTAAT AREILANVPT PENAVLLAVS PDQRAIEVVY GADVKGRGIE
SAAPLGVSAA AASFKEGNLI DGLISAVRVM SAGVSPA