ID   CAS2C_NITEU             Reviewed;          96 AA.
AC   Q82W51;
DT   18-APR-2012, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 84.
DE   RecName: Full=CRISPR-associated endoribonuclease Cas2 3;
DE            EC=3.1.-.-;
GN   Name=cas23; OrderedLocusNames=NE0845;
OS   Nitrosomonas europaea (strain ATCC 19718 / CIP 103999 / KCTC 2705 / NBRC
OS   14298).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales;
OC   Nitrosomonadaceae; Nitrosomonas.
OX   NCBI_TaxID=228410;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 19718 / CIP 103999 / KCTC 2705 / NBRC 14298;
RX   PubMed=12700255; DOI=10.1128/jb.185.9.2759-2773.2003;
RA   Chain P., Lamerdin J.E., Larimer F.W., Regala W., Lao V., Land M.L.,
RA   Hauser L., Hooper A.B., Klotz M.G., Norton J., Sayavedra-Soto L.A.,
RA   Arciero D.M., Hommes N.G., Whittaker M.M., Arp D.J.;
RT   "Complete genome sequence of the ammonia-oxidizing bacterium and obligate
RT   chemolithoautotroph Nitrosomonas europaea.";
RL   J. Bacteriol. 185:2759-2773(2003).
RN   [2]
RP   FUNCTION AS A SSRNA-SPECIFIC ENDORIBONUCLEASE.
RC   STRAIN=ATCC 19718 / CIP 103999 / KCTC 2705 / NBRC 14298;
RX   PubMed=18482976; DOI=10.1074/jbc.m803225200;
RA   Beloglazova N., Brown G., Zimmerman M.D., Proudfoot M., Makarova K.S.,
RA   Kudritska M., Kochinyan S., Wang S., Chruszcz M., Minor W., Koonin E.V.,
RA   Edwards A.M., Savchenko A., Yakunin A.F.;
RT   "A novel family of sequence-specific endoribonucleases associated with the
RT   clustered regularly interspaced short palindromic repeats.";
RL   J. Biol. Chem. 283:20361-20371(2008).
CC   -!- FUNCTION: CRISPR (clustered regularly interspaced short palindromic
CC       repeat), is an adaptive immune system that provides protection against
CC       mobile genetic elements (viruses, transposable elements and conjugative
CC       plasmids). CRISPR clusters contain sequences complementary to
CC       antecedent mobile elements and target invading nucleic acids. CRISPR
CC       clusters are transcribed and processed into CRISPR RNA (crRNA).
CC       Involved in the integration of spacer DNA into the CRISPR cassette (By
CC       similarity). Functions as a ssRNA-specific endoribonuclease.
CC       {ECO:0000250, ECO:0000269|PubMed:18482976}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC   -!- SUBUNIT: Homodimer, forms a heterotetramer with a Cas1 homodimer.
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the CRISPR-associated endoribonuclease Cas2
CC       protein family. {ECO:0000305}.
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DR   EMBL; AL954747; CAD84756.1; -; Genomic_DNA.
DR   RefSeq; WP_011111456.1; NC_004757.1.
DR   AlphaFoldDB; Q82W51; -.
DR   SMR; Q82W51; -.
DR   STRING; 228410.NE0845; -.
DR   EnsemblBacteria; CAD84756; CAD84756; NE0845.
DR   KEGG; neu:NE0845; -.
DR   eggNOG; COG1343; Bacteria.
DR   HOGENOM; CLU_161124_3_1_4; -.
DR   OMA; CYDVNTE; -.
DR   OrthoDB; 1951170at2; -.
DR   PhylomeDB; Q82W51; -.
DR   Proteomes; UP000001416; Chromosome.
DR   GO; GO:0004521; F:endoribonuclease activity; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-UniRule.
DR   GO; GO:0043571; P:maintenance of CRISPR repeat elements; IEA:UniProtKB-UniRule.
DR   CDD; cd09725; Cas2_I_II_III; 1.
DR   HAMAP; MF_01471; Cas2; 1.
DR   InterPro; IPR021127; CRISPR_associated_Cas2.
DR   InterPro; IPR019199; Virulence_VapD/CRISPR_Cas2.
DR   PANTHER; PTHR34405; PTHR34405; 1.
DR   Pfam; PF09827; CRISPR_Cas2; 1.
DR   PIRSF; PIRSF032582; Cas2; 1.
DR   TIGRFAMs; TIGR01573; cas2; 1.
PE   1: Evidence at protein level;
KW   Antiviral defense; Endonuclease; Hydrolase; Magnesium; Metal-binding;
KW   Nuclease; Reference proteome.
FT   CHAIN           1..96
FT                   /note="CRISPR-associated endoribonuclease Cas2 3"
FT                   /id="PRO_0000416946"
FT   BINDING         8
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   96 AA;  11376 MW;  BB7400A1C306637E CRC64;
     MLIIVTYDVS TETRAGRKRL RRVAKLCESI GQRVQKSVFE CRINLMQYEE LERRLLSEID
     EQEDNLRLYR LTEPAELHVK EYGNFKAIDF EGPLTI