ID   Y480_MYCTO              Reviewed;         280 AA.
AC   P9WJ00; L0T5H5; Q11146;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   25-MAY-2022, entry version 33.
DE   RecName: Full=Hydrolase MT0498;
DE            EC=3.5.-.-;
GN   OrderedLocusNames=MT0498;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA   Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA   Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA   Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
CC   -!- SIMILARITY: Belongs to the carbon-nitrogen hydrolase superfamily.
CC       NIT1/NIT2 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAK44721.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AE000516; AAK44721.1; ALT_INIT; Genomic_DNA.
DR   PIR; C70743; C70743.
DR   RefSeq; WP_003402350.1; NZ_KK341227.1.
DR   AlphaFoldDB; P9WJ00; -.
DR   SMR; P9WJ00; -.
DR   EnsemblBacteria; AAK44721; AAK44721; MT0498.
DR   GeneID; 45424441; -.
DR   KEGG; mtc:MT0498; -.
DR   PATRIC; fig|83331.31.peg.527; -.
DR   HOGENOM; CLU_030130_1_2_11; -.
DR   Proteomes; UP000001020; Chromosome.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006807; P:nitrogen compound metabolic process; IEA:InterPro.
DR   Gene3D; 3.60.110.10; -; 1.
DR   InterPro; IPR003010; C-N_Hydrolase.
DR   InterPro; IPR036526; C-N_Hydrolase_sf.
DR   InterPro; IPR001110; UPF0012_CS.
DR   Pfam; PF00795; CN_hydrolase; 1.
DR   SUPFAM; SSF56317; SSF56317; 1.
DR   PROSITE; PS50263; CN_HYDROLASE; 1.
DR   PROSITE; PS01227; UPF0012; 1.
PE   3: Inferred from homology;
KW   Hydrolase.
FT   CHAIN           1..280
FT                   /note="Hydrolase MT0498"
FT                   /id="PRO_0000427916"
FT   DOMAIN          1..251
FT                   /note="CN hydrolase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00054"
FT   ACT_SITE        40
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00054"
FT   ACT_SITE        110
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00054"
FT   ACT_SITE        146
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00054"
SQ   SEQUENCE   280 AA;  28995 MW;  5A63EF070B04A443 CRC64;
     MRIALAQIRS GTDPAANLQL VGKYAGEAAT AGAQLVVFPE ATMCRLGVPL RQVAEPVDGP
     WANGVRRIAT EAGITVIAGM FTPTGDGRVT NTLIAAGPGT PNQPDAHYHK IHLYDAFGFT
     ESRTVAPGRE PVVVVVDGVR VGLTVCYDIR FPALYTELAR RGAQLIAVCA SWGSGPGKLE
     QWTLLARARA LDSMSYVAAA GQADPGDART GVGASSAAPT GVGGSLVASP LGEVVVSAGT
     QPQLLVADID VDNVAAARDR IAVLRNQTDF VQIDKAQSRG