ID   CAS2_CAMJE              Reviewed;         143 AA.
AC   Q0P899;
DT   13-JUN-2012, integrated into UniProtKB/Swiss-Prot.
DT   19-SEP-2006, sequence version 1.
DT   03-AUG-2022, entry version 65.
DE   RecName: Full=CRISPR-associated endoribonuclease Cas2 {ECO:0000255|HAMAP-Rule:MF_01471};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_01471};
GN   Name=cas2 {ECO:0000255|HAMAP-Rule:MF_01471}; OrderedLocusNames=Cj1521c;
OS   Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC
OS   11168).
OC   Bacteria; Proteobacteria; Epsilonproteobacteria; Campylobacterales;
OC   Campylobacteraceae; Campylobacter.
OX   NCBI_TaxID=192222;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700819 / NCTC 11168;
RX   PubMed=10688204; DOI=10.1038/35001088;
RA   Parkhill J., Wren B.W., Mungall K.L., Ketley J.M., Churcher C.M.,
RA   Basham D., Chillingworth T., Davies R.M., Feltwell T., Holroyd S.,
RA   Jagels K., Karlyshev A.V., Moule S., Pallen M.J., Penn C.W., Quail M.A.,
RA   Rajandream M.A., Rutherford K.M., van Vliet A.H.M., Whitehead S.,
RA   Barrell B.G.;
RT   "The genome sequence of the food-borne pathogen Campylobacter jejuni
RT   reveals hypervariable sequences.";
RL   Nature 403:665-668(2000).
CC   -!- FUNCTION: CRISPR (clustered regularly interspaced short palindromic
CC       repeat), is an adaptive immune system that provides protection against
CC       mobile genetic elements (viruses, transposable elements and conjugative
CC       plasmids). CRISPR clusters contain sequences complementary to
CC       antecedent mobile elements and target invading nucleic acids. CRISPR
CC       clusters are transcribed and processed into CRISPR RNA (crRNA).
CC       Functions as a ssRNA-specific endoribonuclease. Involved in the
CC       integration of spacer DNA into the CRISPR cassette. {ECO:0000255|HAMAP-
CC       Rule:MF_01471}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01471};
CC   -!- SUBUNIT: Homodimer, forms a heterotetramer with a Cas1 homodimer.
CC       {ECO:0000255|HAMAP-Rule:MF_01471}.
CC   -!- SIMILARITY: Belongs to the CRISPR-associated endoribonuclease Cas2
CC       protein family. {ECO:0000255|HAMAP-Rule:MF_01471}.
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DR   EMBL; AL111168; CAL35625.1; -; Genomic_DNA.
DR   PIR; D81299; D81299.
DR   RefSeq; WP_002797817.1; NC_002163.1.
DR   RefSeq; YP_002344898.1; NC_002163.1.
DR   AlphaFoldDB; Q0P899; -.
DR   SMR; Q0P899; -.
DR   STRING; 192222.Cj1521c; -.
DR   PaxDb; Q0P899; -.
DR   PRIDE; Q0P899; -.
DR   EnsemblBacteria; CAL35625; CAL35625; Cj1521c.
DR   GeneID; 905810; -.
DR   KEGG; cje:Cj1521c; -.
DR   PATRIC; fig|192222.6.peg.1499; -.
DR   eggNOG; COG3512; Bacteria.
DR   HOGENOM; CLU_150500_1_0_7; -.
DR   OMA; TDKQFGM; -.
DR   Proteomes; UP000000799; Chromosome.
DR   GO; GO:0004521; F:endoribonuclease activity; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-UniRule.
DR   GO; GO:0043571; P:maintenance of CRISPR repeat elements; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_01471; Cas2; 1.
DR   InterPro; IPR021127; CRISPR_associated_Cas2.
DR   InterPro; IPR019199; Virulence_VapD/CRISPR_Cas2.
DR   Pfam; PF09827; CRISPR_Cas2; 1.
DR   TIGRFAMs; TIGR01573; cas2; 1.
PE   3: Inferred from homology;
KW   Antiviral defense; Endonuclease; Hydrolase; Magnesium; Metal-binding;
KW   Nuclease; Reference proteome.
FT   CHAIN           1..143
FT                   /note="CRISPR-associated endoribonuclease Cas2"
FT                   /id="PRO_0000417703"
FT   BINDING         14
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01471"
SQ   SEQUENCE   143 AA;  16854 MW;  DCB34BFFF1293B2B CRC64;
     MIEDKFMRVL LMFDVPTKSK KEQKLASKFR NNLIKLGYFM LQFSVYMRIC KGLSSAKSSI
     ENVKKILPPY GNVRALIITE KQFDKMELLL GGIVFNEKVN NETNLTLFDI DSHGEFKYKN
     SNNEEIQINK KQEKYHQQNL FEF