Y4872_PSEPK
ID Y4872_PSEPK Reviewed; 766 AA.
AC Q88DF3;
DT 24-MAY-2004, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2004, sequence version 2.
DT 03-AUG-2022, entry version 105.
DE RecName: Full=UPF0313 protein PP_4872 {ECO:0000255|HAMAP-Rule:MF_01251};
GN OrderedLocusNames=PP_4872;
OS Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950
OS / KT2440).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=160488;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440;
RX PubMed=12534463; DOI=10.1046/j.1462-2920.2002.00366.x;
RA Nelson K.E., Weinel C., Paulsen I.T., Dodson R.J., Hilbert H.,
RA Martins dos Santos V.A.P., Fouts D.E., Gill S.R., Pop M., Holmes M.,
RA Brinkac L.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Kolonay J.F.,
RA Madupu R., Nelson W.C., White O., Peterson J.D., Khouri H.M., Hance I.,
RA Chris Lee P., Holtzapple E.K., Scanlan D., Tran K., Moazzez A.,
RA Utterback T.R., Rizzo M., Lee K., Kosack D., Moestl D., Wedler H.,
RA Lauber J., Stjepandic D., Hoheisel J., Straetz M., Heim S., Kiewitz C.,
RA Eisen J.A., Timmis K.N., Duesterhoeft A., Tuemmler B., Fraser C.M.;
RT "Complete genome sequence and comparative analysis of the metabolically
RT versatile Pseudomonas putida KT2440.";
RL Environ. Microbiol. 4:799-808(2002).
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01251};
CC Note=Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3
CC cysteines and an exchangeable S-adenosyl-L-methionine.
CC {ECO:0000255|HAMAP-Rule:MF_01251};
CC -!- SIMILARITY: Belongs to the UPF0313 family. {ECO:0000255|HAMAP-
CC Rule:MF_01251}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAN70441.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AE015451; AAN70441.1; ALT_INIT; Genomic_DNA.
DR RefSeq; NP_746977.3; NC_002947.4.
DR RefSeq; WP_010955471.1; NC_002947.4.
DR AlphaFoldDB; Q88DF3; -.
DR STRING; 160488.PP_4872; -.
DR PRIDE; Q88DF3; -.
DR EnsemblBacteria; AAN70441; AAN70441; PP_4872.
DR KEGG; ppu:PP_4872; -.
DR PATRIC; fig|160488.4.peg.5204; -.
DR eggNOG; COG1032; Bacteria.
DR HOGENOM; CLU_018288_2_0_6; -.
DR OMA; TCMYYTG; -.
DR PhylomeDB; Q88DF3; -.
DR Proteomes; UP000000556; Chromosome.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-UniRule.
DR Gene3D; 3.80.30.20; -; 1.
DR HAMAP; MF_01251; UPF0313; 1.
DR InterPro; IPR006638; Elp3/MiaB/NifB.
DR InterPro; IPR020612; Methylthiotransferase_CS.
DR InterPro; IPR007197; rSAM.
DR InterPro; IPR023404; rSAM_horseshoe.
DR InterPro; IPR022946; UPF0313.
DR InterPro; IPR024560; UPF0313_C.
DR InterPro; IPR013704; UPF0313_N.
DR PANTHER; PTHR32331; PTHR32331; 1.
DR Pfam; PF11842; DUF3362; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR Pfam; PF08497; Radical_SAM_N; 1.
DR SFLD; SFLDS00029; Radical_SAM; 1.
DR SFLD; SFLDG01069; UPF0313; 1.
DR SMART; SM00729; Elp3; 1.
DR TIGRFAMs; TIGR03904; SAM_YgiQ; 1.
DR PROSITE; PS51918; RADICAL_SAM; 1.
PE 3: Inferred from homology;
KW 4Fe-4S; Iron; Iron-sulfur; Metal-binding; Reference proteome;
KW S-adenosyl-L-methionine.
FT CHAIN 1..766
FT /note="UPF0313 protein PP_4872"
FT /id="PRO_0000076390"
FT DOMAIN 371..649
FT /note="Radical SAM core"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01266"
FT REGION 670..766
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 677..694
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 721..755
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 385
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_note="4Fe-4S-S-AdoMet"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01251"
FT BINDING 389
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_note="4Fe-4S-S-AdoMet"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01251"
FT BINDING 392
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_note="4Fe-4S-S-AdoMet"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01251"
SQ SEQUENCE 766 AA; 86271 MW; 7670B3AD07893175 CRC64;
MQAAKPLYDY PKYWAECFGP APFLPMSREE MDLLGWDSCD IIIVTGDAYV DHPSFGMAII
GRLLEAQGFR VGIIAQPNWQ SKDDFMKLGE PNLFFGVAAG NMDSMINRYT ADKKIRSDDA
YTPGGLAGSR PDRASLVYSQ RCKEAYKHVP IVLGGIEASL RRIAHYDYWQ DKVRHSILID
ASADILLFGN AERAVVEVAQ RLSNGEKIET ITDVRGTAFV RRDTPQGWYE IDSTRIDRPG
RVDKIINPYV NTQDTQACAI EQAKGDQEDP NEAKVVQILD SPAVTREKSV IRLPSFEKVR
NDPVLYAHAN RVLHLETNPG NARALVQKHG EVDVWFNPPP IPMSTEEMDY VFGMPYARVP
HPAYGKERIP AYEMIRFSVN IMRGCFGGCT FCSITEHEGR IIQNRSHESI LHEIEEMRDK
VPGFTGVVSD LGGPTANMYR IACKSPEIEK YCRKPSCVFP GICENLNTDH SSLIELYRKA
RALPGVKKIL IASGLRYDLA VESPEYVKEL VTHHVGGYLK IAPEHTERGP LDKMMKPGIG
TYDRFKRMFE KFSKEAGKEQ YLIPYFIAAH PGTTDEDMMN LALWLKGNGF RADQVQAFYP
SPMASATAMY HSGKNPLRKV TYKSEGVEIV KSDEQRRLHK AFLRYHDPKG WPMLREALQR
MGRADLIGPG KHQLIPLHQP QTDTYQSARR KNSTPAGSHK VGKDQKILTQ HTGLPPRGSD
GSKPWDKREK AKAEAFARNQ QAAKERKEAS KGGKGNKKPR QPVIPR
