ID   CAS2_GEOSL              Reviewed;          95 AA.
AC   Q74H35;
DT   13-JUN-2012, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 84.
DE   RecName: Full=CRISPR-associated endoribonuclease Cas2 {ECO:0000255|HAMAP-Rule:MF_01471};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_01471};
GN   Name=cas2 {ECO:0000255|HAMAP-Rule:MF_01471}; OrderedLocusNames=GSU0058;
OS   Geobacter sulfurreducens (strain ATCC 51573 / DSM 12127 / PCA).
OC   Bacteria; Proteobacteria; Deltaproteobacteria; Desulfuromonadales;
OC   Geobacteraceae; Geobacter.
OX   NCBI_TaxID=243231;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 51573 / DSM 12127 / PCA;
RX   PubMed=14671304; DOI=10.1126/science.1088727;
RA   Methe B.A., Nelson K.E., Eisen J.A., Paulsen I.T., Nelson W.C.,
RA   Heidelberg J.F., Wu D., Wu M., Ward N.L., Beanan M.J., Dodson R.J.,
RA   Madupu R., Brinkac L.M., Daugherty S.C., DeBoy R.T., Durkin A.S.,
RA   Gwinn M.L., Kolonay J.F., Sullivan S.A., Haft D.H., Selengut J.,
RA   Davidsen T.M., Zafar N., White O., Tran B., Romero C., Forberger H.A.,
RA   Weidman J.F., Khouri H.M., Feldblyum T.V., Utterback T.R., Van Aken S.E.,
RA   Lovley D.R., Fraser C.M.;
RT   "Genome of Geobacter sulfurreducens: metal reduction in subsurface
RT   environments.";
RL   Science 302:1967-1969(2003).
CC   -!- FUNCTION: CRISPR (clustered regularly interspaced short palindromic
CC       repeat), is an adaptive immune system that provides protection against
CC       mobile genetic elements (viruses, transposable elements and conjugative
CC       plasmids). CRISPR clusters contain sequences complementary to
CC       antecedent mobile elements and target invading nucleic acids. CRISPR
CC       clusters are transcribed and processed into CRISPR RNA (crRNA).
CC       Functions as a ssRNA-specific endoribonuclease. Involved in the
CC       integration of spacer DNA into the CRISPR cassette. {ECO:0000255|HAMAP-
CC       Rule:MF_01471}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01471};
CC   -!- SUBUNIT: Homodimer, forms a heterotetramer with a Cas1 homodimer.
CC       {ECO:0000255|HAMAP-Rule:MF_01471}.
CC   -!- SIMILARITY: Belongs to the CRISPR-associated endoribonuclease Cas2
CC       protein family. {ECO:0000255|HAMAP-Rule:MF_01471}.
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DR   EMBL; AE017180; AAR33393.1; -; Genomic_DNA.
DR   RefSeq; NP_951120.1; NC_002939.5.
DR   RefSeq; WP_010940736.1; NC_002939.5.
DR   PDB; 7MI4; EM; 3.20 A; E/F=1-95.
DR   PDB; 7MI5; EM; 3.57 A; E/F=1-95.
DR   PDB; 7MI9; EM; 3.89 A; E/F=1-95.
DR   PDB; 7MIB; EM; 5.80 A; E/F=1-95.
DR   PDB; 7MID; EM; 3.56 A; C/D=1-95.
DR   PDBsum; 7MI4; -.
DR   PDBsum; 7MI5; -.
DR   PDBsum; 7MI9; -.
DR   PDBsum; 7MIB; -.
DR   PDBsum; 7MID; -.
DR   AlphaFoldDB; Q74H35; -.
DR   SMR; Q74H35; -.
DR   STRING; 243231.GSU0058; -.
DR   DNASU; 2688484; -.
DR   EnsemblBacteria; AAR33393; AAR33393; GSU0058.
DR   KEGG; gsu:GSU0058; -.
DR   PATRIC; fig|243231.5.peg.58; -.
DR   eggNOG; COG1343; Bacteria.
DR   HOGENOM; CLU_161124_3_1_7; -.
DR   InParanoid; Q74H35; -.
DR   OMA; LSVFQCR; -.
DR   Proteomes; UP000000577; Chromosome.
DR   GO; GO:0004521; F:endoribonuclease activity; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-UniRule.
DR   GO; GO:0043571; P:maintenance of CRISPR repeat elements; IEA:UniProtKB-UniRule.
DR   CDD; cd09725; Cas2_I_II_III; 1.
DR   HAMAP; MF_01471; Cas2; 1.
DR   InterPro; IPR021127; CRISPR_associated_Cas2.
DR   InterPro; IPR019199; Virulence_VapD/CRISPR_Cas2.
DR   PANTHER; PTHR34405; PTHR34405; 1.
DR   Pfam; PF09827; CRISPR_Cas2; 1.
DR   TIGRFAMs; TIGR01573; cas2; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Antiviral defense; Endonuclease; Hydrolase; Magnesium;
KW   Metal-binding; Nuclease; Reference proteome.
FT   CHAIN           1..95
FT                   /note="CRISPR-associated endoribonuclease Cas2"
FT                   /id="PRO_0000417715"
FT   BINDING         10
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01471"
FT   STRAND          3..10
FT                   /evidence="ECO:0007829|PDB:7MI4"
FT   HELIX           14..27
FT                   /evidence="ECO:0007829|PDB:7MI4"
FT   STRAND          28..32
FT                   /evidence="ECO:0007829|PDB:7MI4"
FT   STRAND          35..40
FT                   /evidence="ECO:0007829|PDB:7MI4"
FT   HELIX           42..51
FT                   /evidence="ECO:0007829|PDB:7MI4"
FT   TURN            52..54
FT                   /evidence="ECO:0007829|PDB:7MI4"
FT   TURN            58..60
FT                   /evidence="ECO:0007829|PDB:7MI4"
FT   STRAND          62..73
FT                   /evidence="ECO:0007829|PDB:7MI4"
FT   HELIX           75..77
FT                   /evidence="ECO:0007829|PDB:7MI4"
FT   STRAND          78..83
FT                   /evidence="ECO:0007829|PDB:7MI4"
SQ   SEQUENCE   95 AA;  11172 MW;  542FA393F983EC3B CRC64;
     MEHLYIVSYD IRNQRRWRRL FKTMHGFGCW LQLSVFQCRL DRIRIIKMEA AINEIVNHAE
     DHVLILDLGP AENVKPKVSS IGKTFDPILR QAVIV