ID   Y4928_PSEAE             Reviewed;         747 AA.
AC   Q9HUN6;
DT   24-MAY-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 103.
DE   RecName: Full=UPF0313 protein PA4928 {ECO:0000255|HAMAP-Rule:MF_01251};
GN   OrderedLocusNames=PA4928;
OS   Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS   14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208964;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA   Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT   pathogen.";
RL   Nature 406:959-964(2000).
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01251};
CC       Note=Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3
CC       cysteines and an exchangeable S-adenosyl-L-methionine.
CC       {ECO:0000255|HAMAP-Rule:MF_01251};
CC   -!- SIMILARITY: Belongs to the UPF0313 family. {ECO:0000255|HAMAP-
CC       Rule:MF_01251}.
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DR   EMBL; AE004091; AAG08313.1; -; Genomic_DNA.
DR   PIR; B83029; B83029.
DR   RefSeq; NP_253615.1; NC_002516.2.
DR   RefSeq; WP_003100007.1; NZ_QZGE01000002.1.
DR   AlphaFoldDB; Q9HUN6; -.
DR   SMR; Q9HUN6; -.
DR   STRING; 287.DR97_2279; -.
DR   PaxDb; Q9HUN6; -.
DR   EnsemblBacteria; AAG08313; AAG08313; PA4928.
DR   GeneID; 879696; -.
DR   KEGG; pae:PA4928; -.
DR   PATRIC; fig|208964.12.peg.5161; -.
DR   PseudoCAP; PA4928; -.
DR   HOGENOM; CLU_018288_2_0_6; -.
DR   InParanoid; Q9HUN6; -.
DR   OMA; TCMYYTG; -.
DR   PhylomeDB; Q9HUN6; -.
DR   BioCyc; PAER208964:G1FZ6-5042-MON; -.
DR   Proteomes; UP000002438; Chromosome.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.80.30.20; -; 1.
DR   HAMAP; MF_01251; UPF0313; 1.
DR   InterPro; IPR006638; Elp3/MiaB/NifB.
DR   InterPro; IPR020612; Methylthiotransferase_CS.
DR   InterPro; IPR007197; rSAM.
DR   InterPro; IPR023404; rSAM_horseshoe.
DR   InterPro; IPR022946; UPF0313.
DR   InterPro; IPR024560; UPF0313_C.
DR   InterPro; IPR013704; UPF0313_N.
DR   PANTHER; PTHR32331; PTHR32331; 1.
DR   Pfam; PF11842; DUF3362; 1.
DR   Pfam; PF04055; Radical_SAM; 1.
DR   Pfam; PF08497; Radical_SAM_N; 1.
DR   SFLD; SFLDS00029; Radical_SAM; 1.
DR   SFLD; SFLDG01069; UPF0313; 1.
DR   SMART; SM00729; Elp3; 1.
DR   TIGRFAMs; TIGR03904; SAM_YgiQ; 1.
DR   PROSITE; PS51918; RADICAL_SAM; 1.
PE   3: Inferred from homology;
KW   4Fe-4S; Iron; Iron-sulfur; Metal-binding; Reference proteome;
KW   S-adenosyl-L-methionine.
FT   CHAIN           1..747
FT                   /note="UPF0313 protein PA4928"
FT                   /id="PRO_0000076389"
FT   DOMAIN          371..640
FT                   /note="Radical SAM core"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01266"
FT   REGION          670..747
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        680..694
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         385
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01251"
FT   BINDING         389
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01251"
FT   BINDING         392
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01251"
SQ   SEQUENCE   747 AA;  83930 MW;  CE2DA24F430AC8FC CRC64;
     MQAAKPLFDY PKYWAECFGP APFLPMSREE MDQLGWDSCD IIIVTGDAYV DHPSFGMAII
     GRLLEAQGFR VGIIAQPDWQ SKDDFMKLGE PNLFFGVAAG NMDSMINRYT ADKKVRSDDA
     YTPGGLAGKR PDRASLVYSQ RCKEAYSHVP VILGGIEASL RRIAHYDYWQ DKVRRSILMD
     ATADILLYGN AERAVVEIAQ RLAQGELVSA ITDVRGTAFV RRDTPEGWFE IDSTRIDRPG
     KIDKIINPYV NTQDTAACAI EQEKGAQEDP QEAKVVQLLA NPRLTREKTV IRLPSFEKVR
     NDPVLYAHAN RVLHLETNPG NARALVQKHG EVDVWFNPPP IPMSTEEMDY VFGMPYARVP
     HPAYGKAKIP AYEMIRFSVN IMRGCFGGCT FCSITEHEGR IIQNRSHDSI IREIEEMRDK
     VPGFTGVVSD LGGPTANMYR IACKSPDIER HCRKPSCVFP GICENLDTDH SSLIELYRKA
     RALPGVKKIL IASGLRYDLA VESPEYVKEL VTHHVGGYLK IAPEHTERGP LDKMMKPGIG
     TYDRFKQMFE KFSKEAGKEQ YLIPYFIAAH PGTTDEDMMN LALWLKRNGF RADQVQAFYP
     SPMATATAMY HSGKNPLRKV TYKSDGVTIV KSDQQRRLHK AFLRYHDPKG WPLLREALER
     MGRADLIGNG KHHLVPTYQP DTGEYQSARR KNSTPAGSKK AGKLLTQHTG LPPRASDGGK
     PWSKGQQNKA TAFAKGKKKS RQPNIPR