ID   Y4928_PSESM             Reviewed;         771 AA.
AC   Q87VK8;
DT   24-MAY-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 101.
DE   RecName: Full=UPF0313 protein PSPTO_4928 {ECO:0000255|HAMAP-Rule:MF_01251};
GN   OrderedLocusNames=PSPTO_4928;
OS   Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=223283;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-871 / DC3000;
RX   PubMed=12928499; DOI=10.1073/pnas.1731982100;
RA   Buell C.R., Joardar V., Lindeberg M., Selengut J., Paulsen I.T.,
RA   Gwinn M.L., Dodson R.J., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R.,
RA   Daugherty S.C., Brinkac L.M., Beanan M.J., Haft D.H., Nelson W.C.,
RA   Davidsen T.M., Zafar N., Zhou L., Liu J., Yuan Q., Khouri H.M.,
RA   Fedorova N.B., Tran B., Russell D., Berry K.J., Utterback T.R.,
RA   Van Aken S.E., Feldblyum T.V., D'Ascenzo M., Deng W.-L., Ramos A.R.,
RA   Alfano J.R., Cartinhour S., Chatterjee A.K., Delaney T.P., Lazarowitz S.G.,
RA   Martin G.B., Schneider D.J., Tang X., Bender C.L., White O., Fraser C.M.,
RA   Collmer A.;
RT   "The complete genome sequence of the Arabidopsis and tomato pathogen
RT   Pseudomonas syringae pv. tomato DC3000.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:10181-10186(2003).
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01251};
CC       Note=Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3
CC       cysteines and an exchangeable S-adenosyl-L-methionine.
CC       {ECO:0000255|HAMAP-Rule:MF_01251};
CC   -!- SIMILARITY: Belongs to the UPF0313 family. {ECO:0000255|HAMAP-
CC       Rule:MF_01251}.
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DR   EMBL; AE016853; AAO58356.1; -; Genomic_DNA.
DR   RefSeq; NP_794661.1; NC_004578.1.
DR   RefSeq; WP_005763035.1; NC_004578.1.
DR   AlphaFoldDB; Q87VK8; -.
DR   STRING; 223283.PSPTO_4928; -.
DR   PRIDE; Q87VK8; -.
DR   EnsemblBacteria; AAO58356; AAO58356; PSPTO_4928.
DR   GeneID; 1186611; -.
DR   KEGG; pst:PSPTO_4928; -.
DR   PATRIC; fig|223283.9.peg.5042; -.
DR   eggNOG; COG1032; Bacteria.
DR   HOGENOM; CLU_018288_2_0_6; -.
DR   OMA; TCMYYTG; -.
DR   OrthoDB; 229299at2; -.
DR   PhylomeDB; Q87VK8; -.
DR   Proteomes; UP000002515; Chromosome.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.80.30.20; -; 1.
DR   HAMAP; MF_01251; UPF0313; 1.
DR   InterPro; IPR006638; Elp3/MiaB/NifB.
DR   InterPro; IPR020612; Methylthiotransferase_CS.
DR   InterPro; IPR007197; rSAM.
DR   InterPro; IPR023404; rSAM_horseshoe.
DR   InterPro; IPR022946; UPF0313.
DR   InterPro; IPR024560; UPF0313_C.
DR   InterPro; IPR013704; UPF0313_N.
DR   PANTHER; PTHR32331; PTHR32331; 1.
DR   Pfam; PF11842; DUF3362; 1.
DR   Pfam; PF04055; Radical_SAM; 1.
DR   Pfam; PF08497; Radical_SAM_N; 1.
DR   SFLD; SFLDS00029; Radical_SAM; 1.
DR   SFLD; SFLDG01069; UPF0313; 1.
DR   SMART; SM00729; Elp3; 1.
DR   TIGRFAMs; TIGR03904; SAM_YgiQ; 1.
DR   PROSITE; PS51918; RADICAL_SAM; 1.
PE   3: Inferred from homology;
KW   4Fe-4S; Iron; Iron-sulfur; Metal-binding; Reference proteome;
KW   S-adenosyl-L-methionine.
FT   CHAIN           1..771
FT                   /note="UPF0313 protein PSPTO_4928"
FT                   /id="PRO_0000076391"
FT   DOMAIN          371..649
FT                   /note="Radical SAM core"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01266"
FT   REGION          683..771
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        705..719
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        724..758
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         385
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01251"
FT   BINDING         389
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01251"
FT   BINDING         392
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /ligand_note="4Fe-4S-S-AdoMet"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01251"
SQ   SEQUENCE   771 AA;  86277 MW;  37ADF6FCA035A353 CRC64;
     MQSAKPLFDY PKYWAECFGP APFLPMSREE MDLLGWDSCD IIIVTGDAYV DHPSFGMAII
     GRLLESQGFR VGIIAQPNWQ SKDDFMKLGE PNLFFGVAAG NMDSMINRYT ADKKIRSDDA
     YTPGGMAGKR PDRASLVYSQ RCKEAYKHVP IVLGGIEASL RRIAHYDYWQ DKVRNSILID
     ACADILLYGN AERAIVEVAQ RLSYGHKIED ITDVRGTAFI RRDTPQGWYE VDSTRIDRPG
     KVDKIINPYV NTQDTQACAI EQEKGNVEDP NEAKVVQILA SPRMTRDKTV IRLPSVEKVR
     NDAVLYAHAN RVLHLETNPG NARALVQKHG EVDVWFNPPP IPMTTDEMDY VFGMPYARVP
     HPAYGKEKIP AYDMIRFSVN IMRGCFGGCT FCSITEHEGR IIQNRSEESI IREIEEIRDK
     VPGFTGVISD LGGPTANMYR IACKSPEIES ACRKPSCVFP GICPNLNTDH SSLIQLYRSA
     RALPGVKKIL IASGLRYDLA VESPEYVKEL VTHHVGGYLK IAPEHTEEGP LNQMMKPGIG
     SYDKFKRMFE KYTKEAGKEQ YLIPYFIAAH PGTTDEDMMN LALWLKGNGF RADQVQAFYP
     SPMATATAMY HSGKNPLRKV TYKSDAVTIV KSEEQRRLHK AFLRYHDPKG WPMLRAALER
     MGRADLIGPG KDQLIPLHQP ATDTYQSARR KNSTPAGSHK VGKDPKTTLI QTQHTGLPPR
     GSDGSKPWDK REEAKAAAMA RNKQAAKERM DAAKGGKGKG GKPARKPVVP R