ID   CAS2_LACRG              Reviewed;         101 AA.
AC   C7TEQ4; C8UW90;
DT   13-JUN-2012, integrated into UniProtKB/Swiss-Prot.
DT   13-OCT-2009, sequence version 1.
DT   03-AUG-2022, entry version 61.
DE   RecName: Full=CRISPR-associated endoribonuclease Cas2 {ECO:0000255|HAMAP-Rule:MF_01471};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_01471};
GN   Name=cas2 {ECO:0000255|HAMAP-Rule:MF_01471};
GN   OrderedLocusNames=LGG_02202, LRHM_2117;
OS   Lacticaseibacillus rhamnosus (strain ATCC 53103 / LMG 18243 / GG)
OS   (Lactobacillus rhamnosus).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC   Lacticaseibacillus.
OX   NCBI_TaxID=568703;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 53103 / LMG 18243 / GG;
RX   PubMed=19805152; DOI=10.1073/pnas.0908876106;
RA   Kankainen M., Paulin L., Tynkkynen S., von Ossowski I., Reunanen J.,
RA   Partanen P., Satokari R., Vesterlund S., Hendrickx A.P., Lebeer S.,
RA   De Keersmaecker S.C., Vanderleyden J., Hamalainen T., Laukkanen S.,
RA   Salovuori N., Ritari J., Alatalo E., Korpela R., Mattila-Sandholm T.,
RA   Lassig A., Hatakka K., Kinnunen K.T., Karjalainen H., Saxelin M.,
RA   Laakso K., Surakka A., Palva A., Salusjarvi T., Auvinen P., de Vos W.M.;
RT   "Comparative genomic analysis of Lactobacillus rhamnosus GG reveals pili
RT   containing a human- mucus binding protein.";
RL   Proc. Natl. Acad. Sci. U.S.A. 106:17193-17198(2009).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 53103 / LMG 18243 / GG;
RX   PubMed=19820099; DOI=10.1128/jb.01287-09;
RA   Morita H., Toh H., Oshima K., Murakami M., Taylor T.D., Igimi S.,
RA   Hattori M.;
RT   "Complete genome sequence of the probiotic Lactobacillus rhamnosus ATCC
RT   53103.";
RL   J. Bacteriol. 191:7630-7631(2009).
CC   -!- FUNCTION: CRISPR (clustered regularly interspaced short palindromic
CC       repeat), is an adaptive immune system that provides protection against
CC       mobile genetic elements (viruses, transposable elements and conjugative
CC       plasmids). CRISPR clusters contain sequences complementary to
CC       antecedent mobile elements and target invading nucleic acids. CRISPR
CC       clusters are transcribed and processed into CRISPR RNA (crRNA).
CC       Functions as a ssRNA-specific endoribonuclease. Involved in the
CC       integration of spacer DNA into the CRISPR cassette. {ECO:0000255|HAMAP-
CC       Rule:MF_01471}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01471};
CC   -!- SUBUNIT: Homodimer, forms a heterotetramer with a Cas1 homodimer.
CC       {ECO:0000255|HAMAP-Rule:MF_01471}.
CC   -!- SIMILARITY: Belongs to the CRISPR-associated endoribonuclease Cas2
CC       protein family. {ECO:0000255|HAMAP-Rule:MF_01471}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAI42644.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; FM179322; CAR88097.1; -; Genomic_DNA.
DR   EMBL; AP011548; BAI42644.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; WP_015765030.1; NZ_CP031290.1.
DR   AlphaFoldDB; C7TEQ4; -.
DR   SMR; C7TEQ4; -.
DR   KEGG; lrg:LRHM_2117; -.
DR   KEGG; lrh:LGG_02202; -.
DR   PATRIC; fig|568703.30.peg.2213; -.
DR   HOGENOM; CLU_150500_1_0_9; -.
DR   OMA; TDKQFGM; -.
DR   Proteomes; UP000002067; Chromosome.
DR   GO; GO:0004521; F:endoribonuclease activity; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-UniRule.
DR   GO; GO:0043571; P:maintenance of CRISPR repeat elements; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_01471; Cas2; 1.
DR   InterPro; IPR021127; CRISPR_associated_Cas2.
DR   InterPro; IPR019199; Virulence_VapD/CRISPR_Cas2.
DR   Pfam; PF09827; CRISPR_Cas2; 1.
DR   TIGRFAMs; TIGR01573; cas2; 1.
PE   3: Inferred from homology;
KW   Antiviral defense; Endonuclease; Hydrolase; Magnesium; Metal-binding;
KW   Nuclease.
FT   CHAIN           1..101
FT                   /note="CRISPR-associated endoribonuclease Cas2"
FT                   /id="PRO_0000417716"
FT   BINDING         8
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01471"
SQ   SEQUENCE   101 AA;  11874 MW;  E246CD6752F92AE7 CRC64;
     MRLMIMFDLP VATSKDRRNY RRFRRALIDE GFLMIQYSVY VRVCKTKKSA AYMEQRIATV
     KPPTGIVQTL MVTEAQYQSM HFMVGTEKQD IRNSADRTVM I