CAS2_METED
ID CAS2_METED Reviewed; 95 AA.
AC C7CFQ2;
DT 13-JUN-2012, integrated into UniProtKB/Swiss-Prot.
DT 22-SEP-2009, sequence version 1.
DT 03-AUG-2022, entry version 46.
DE RecName: Full=CRISPR-associated endoribonuclease Cas2 {ECO:0000255|HAMAP-Rule:MF_01471};
DE EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_01471};
GN Name=cas2 {ECO:0000255|HAMAP-Rule:MF_01471}; OrderedLocusNames=METDI1369;
OS Methylorubrum extorquens (strain DSM 6343 / CIP 106787 / DM4)
OS (Methylobacterium extorquens).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Methylobacteriaceae; Methylorubrum.
OX NCBI_TaxID=661410;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 6343 / CIP 106787 / DM4;
RX PubMed=19440302; DOI=10.1371/journal.pone.0005584;
RA Vuilleumier S., Chistoserdova L., Lee M.-C., Bringel F., Lajus A., Zhou Y.,
RA Gourion B., Barbe V., Chang J., Cruveiller S., Dossat C., Gillett W.,
RA Gruffaz C., Haugen E., Hourcade E., Levy R., Mangenot S., Muller E.,
RA Nadalig T., Pagni M., Penny C., Peyraud R., Robinson D.G., Roche D.,
RA Rouy Z., Saenampechek C., Salvignol G., Vallenet D., Wu Z., Marx C.J.,
RA Vorholt J.A., Olson M.V., Kaul R., Weissenbach J., Medigue C.,
RA Lidstrom M.E.;
RT "Methylobacterium genome sequences: a reference blueprint to investigate
RT microbial metabolism of C1 compounds from natural and industrial sources.";
RL PLoS ONE 4:E5584-E5584(2009).
CC -!- FUNCTION: CRISPR (clustered regularly interspaced short palindromic
CC repeat), is an adaptive immune system that provides protection against
CC mobile genetic elements (viruses, transposable elements and conjugative
CC plasmids). CRISPR clusters contain sequences complementary to
CC antecedent mobile elements and target invading nucleic acids. CRISPR
CC clusters are transcribed and processed into CRISPR RNA (crRNA).
CC Functions as a ssRNA-specific endoribonuclease. Involved in the
CC integration of spacer DNA into the CRISPR cassette. {ECO:0000255|HAMAP-
CC Rule:MF_01471}.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01471};
CC -!- SUBUNIT: Homodimer, forms a heterotetramer with a Cas1 homodimer.
CC {ECO:0000255|HAMAP-Rule:MF_01471}.
CC -!- SIMILARITY: Belongs to the CRISPR-associated endoribonuclease Cas2
CC protein family. {ECO:0000255|HAMAP-Rule:MF_01471}.
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DR EMBL; FP103042; CAX22978.1; -; Genomic_DNA.
DR AlphaFoldDB; C7CFQ2; -.
DR SMR; C7CFQ2; -.
DR EnsemblBacteria; CAX22978; CAX22978; METD_I1369.
DR KEGG; mdi:METDI1369; -.
DR HOGENOM; CLU_179928_2_0_5; -.
DR Proteomes; UP000008070; Chromosome.
DR GO; GO:0004521; F:endoribonuclease activity; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-UniRule.
DR GO; GO:0043571; P:maintenance of CRISPR repeat elements; IEA:UniProtKB-UniRule.
DR HAMAP; MF_01471; Cas2; 1.
DR InterPro; IPR021127; CRISPR_associated_Cas2.
DR InterPro; IPR019199; Virulence_VapD/CRISPR_Cas2.
DR Pfam; PF09827; CRISPR_Cas2; 1.
DR TIGRFAMs; TIGR01573; cas2; 1.
PE 3: Inferred from homology;
KW Antiviral defense; Endonuclease; Hydrolase; Magnesium; Metal-binding;
KW Nuclease.
FT CHAIN 1..95
FT /note="CRISPR-associated endoribonuclease Cas2"
FT /id="PRO_0000417720"
FT BINDING 9
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01471"
SQ SEQUENCE 95 AA; 11138 MW; 028CD41DF92B1031 CRC64;
MAVFLIAYDL VNERRGTHDY QPLWDELKRL GAHRTQFSLW LVSANNTTAE VRQHFQQFVD
SNDRIWVTRL RKSQYDYANA IGGTNNWLSN NPPEA
