ID   Y4A0_ENCCU              Reviewed;        1649 AA.
AC   Q8SS35;
DT   01-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   29-MAY-2013, sequence version 2.
DT   03-AUG-2022, entry version 95.
DE   RecName: Full=Probable myosin heavy chain ECU04_1000;
GN   OrderedLocusNames=ECU04_1000;
OS   Encephalitozoon cuniculi (strain GB-M1) (Microsporidian parasite).
OC   Eukaryota; Fungi; Fungi incertae sedis; Microsporidia; Unikaryonidae;
OC   Encephalitozoon.
OX   NCBI_TaxID=284813;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=GB-M1;
RX   PubMed=11719806; DOI=10.1038/35106579;
RA   Katinka M.D., Duprat S., Cornillot E., Metenier G., Thomarat F.,
RA   Prensier G., Barbe V., Peyretaillade E., Brottier P., Wincker P.,
RA   Delbac F., El Alaoui H., Peyret P., Saurin W., Gouy M., Weissenbach J.,
RA   Vivares C.P.;
RT   "Genome sequence and gene compaction of the eukaryote parasite
RT   Encephalitozoon cuniculi.";
RL   Nature 414:450-453(2001).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=GB-M1;
RX   PubMed=20003517; DOI=10.1186/1471-2164-10-607;
RA   Peyretaillade E., Goncalves O., Terrat S., Dugat-Bony E., Wincker P.,
RA   Cornman R.S., Evans J.D., Delbac F., Peyret P.;
RT   "Identification of transcriptional signals in Encephalitozoon cuniculi
RT   widespread among Microsporidia phylum: support for accurate structural
RT   genome annotation.";
RL   BMC Genomics 10:607-607(2009).
RN   [3]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], DEVELOPMENTAL
RP   STAGE, AND SUBCELLULAR LOCATION.
RX   PubMed=16691553; DOI=10.1002/pmic.200500796;
RA   Brosson D., Kuhn L., Delbac F., Garin J., Vivares C.P., Texier C.;
RT   "Proteomic analysis of the eukaryotic parasite Encephalitozoon cuniculi
RT   (microsporidia): a reference map for proteins expressed in late sporogonial
RT   stages.";
RL   Proteomics 6:3625-3635(2006).
CC   -!- FUNCTION: Cellular myosin that appears to play a role in cytokinesis,
CC       cell shape, and specialized functions such as secretion and capping.
CC       {ECO:0000250}.
CC   -!- DEVELOPMENTAL STAGE: Expressed in late sporogonial stages.
CC       {ECO:0000269|PubMed:16691553}.
CC   -!- SIMILARITY: Belongs to the TRAFAC class myosin-kinesin ATPase
CC       superfamily. Myosin family. {ECO:0000305}.
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DR   EMBL; AL590444; CAD25287.2; -; Genomic_DNA.
DR   RefSeq; NP_584783.1; NM_001041133.1.
DR   AlphaFoldDB; Q8SS35; -.
DR   SMR; Q8SS35; -.
DR   STRING; 284813.Q8SS35; -.
DR   PRIDE; Q8SS35; -.
DR   GeneID; 858931; -.
DR   KEGG; ecu:ECU04_1000; -.
DR   VEuPathDB; MicrosporidiaDB:ECU04_1000; -.
DR   HOGENOM; CLU_000192_7_14_1; -.
DR   InParanoid; Q8SS35; -.
DR   OrthoDB; 119761at2759; -.
DR   Proteomes; UP000000819; Chromosome IV.
DR   GO; GO:0016459; C:myosin complex; IEA:UniProtKB-KW.
DR   GO; GO:0051015; F:actin filament binding; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003774; F:cytoskeletal motor activity; IEA:InterPro.
DR   Gene3D; 2.30.30.360; -; 1.
DR   Gene3D; 3.40.850.10; -; 1.
DR   InterPro; IPR036961; Kinesin_motor_dom_sf.
DR   InterPro; IPR001609; Myosin_head_motor_dom.
DR   InterPro; IPR004009; Myosin_N.
DR   InterPro; IPR008989; Myosin_S1_N.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF00063; Myosin_head; 1.
DR   PRINTS; PR00193; MYOSINHEAVY.
DR   SMART; SM00242; MYSc; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS51456; MYOSIN_MOTOR; 1.
DR   PROSITE; PS51844; SH3_LIKE; 1.
PE   1: Evidence at protein level;
KW   Actin-binding; ATP-binding; Coiled coil; Motor protein; Myosin;
KW   Nucleotide-binding; Reference proteome.
FT   CHAIN           1..1649
FT                   /note="Probable myosin heavy chain ECU04_1000"
FT                   /id="PRO_0000383331"
FT   DOMAIN          1..49
FT                   /note="Myosin N-terminal SH3-like"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01190"
FT   DOMAIN          53..724
FT                   /note="Myosin motor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00782"
FT   REGION          594..616
FT                   /note="Actin-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00782"
FT   COILED          793..1614
FT                   /evidence="ECO:0000255"
FT   BINDING         146..153
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00782"
SQ   SEQUENCE   1649 AA;  193288 MW;  FCFF7BA673B391DE CRC64;
     MEKKWVWAPS SKEAYVCGFV VKEEGDVLEI DCRGVIVRHK SCEVFRMNPP KFDMVDDLAE
     LSYLNEPGVL HNLRRRYQNG RIYTYSGLFL LAINPYKDLR IYGEKDARKY TLSKKYELEP
     HIFAVANEAY RLMLSNRENQ SILITGESGA GKTENTKRVV EFLAMVGGCK GMEVSIDRQI
     IDANPILEAF GNAQTVKNDN SSRFGKFIKI KFNGGNICGA HIEKYLLEKS RVTSQNRNER
     NYHIFYQLLG CDDQMLKKQL FLDGEPKDYR FLKDSRFKIP DVDDAKEFRS LRESMRVLGI
     GEEEQIGYFK IVSAILHLGN IEFREKDGAA EIANLDVAEK ACKLLSIPLA EFIKRLIHPV
     IKAGNEYVAH SRSREQALKI VDGLSRILYD KMFEGVIDRI NMSLDSPHKG NFIGVLDIAG
     FEIFEKNSFE QLCINYTNEK LQQFFNHHMF ILEQEVYRQE NIEWDFIDFG LDLQPTIDLI
     EKSNPIGILS YLDEECVMPM ATEKTFLGKL MKNIRDEKFE VDKIRDAFVL NHYAGDVEYT
     VDDWLSKNKD SHSEALTSLI RASGSELVSR LSLNEEAVKK GFFRTVSQKH KEQLASLMSE
     LRRTNPHFVR CIIPNLEKSG EHLDNGIVLG QLKCNGVLEG IRISRQGFPS RMGHREFVQR
     YRIMMKEKIL VDESWDEGVC MELYKEIGGK ILSEIGISTS QYRLGRTKVF FRQGVLADIE
     DMRDVKVSEV VKEIQALIRR RLAFRKYNQA QRRMQGILVI QRNGRICCDL QRWNWWRLYL
     KIKPLLDVRK RDGEMKEKEA MIQEYARMLD AEKSRREEVE DMLKAMSLKR ELLEKSVEDE
     KRFSMEKDEL LMALRYKSDE TAQELEKARK EVFDGHEERK MWETRVNEVA IQLEEKDSEI
     LRLRREVSEQ KGALSQQEKE ICSLREEVVS KLSEKDAMVE KMLRERDSEV QALKEKVKEK
     DAEVERILEG MKRMEREGEE RNRMLKENES TIDELRTRCL NMKRWKDEYA ELREDYEALQ
     KKLKDEVEDM QVENDRLHNE IRKISKEREE LGRMQKKLLD DLEFERNRGS KLEKAFQELR
     GEYEAVEGQL QKERQFRDST QESLLEKTRG LERRVKSLNE KLRREEMANR QLMSEKDEMY
     REIHVLQQSK LDEIFDREAG FNSIKKNLQM EIQRLEMENQ RLSVDLMEAK CMGEASEESI
     SATERFCGML EEERKKRKEI EYQASEHENR NVILSSEVEM LREMVEMERR SKEEVIRGHE
     KETGLCKAIA GVRKEVEDLG NEIDMAIEGF NGMYLNVLDG YKRDLKECKE QVMSKEQVIE
     ELNGRIVRLG REVEERKEIE EEMSRKVHGL MKQYNGVMND FSLLSTKCSS LERTVSEKEE
     EIKGYSERCS EYDKRFEGLV CRVDEEIENL RRSDEERRRC VEKLESGLNG SIAGIRKLDE
     RYKARIEECA QSVLDGERRK LKAMEELCEQ LSKKLGELEE EHQGLLDEKM KGLLRIEQLE
     GELCAFRESE VHRQDMISMY ESEISTLKRC TRFKDEVLGS LSGERNPVVV HVSDKEKCQV
     LDRKRMTVEH ELARANDERQ SLMAINKKLR EEVEKLRGEI DAGRSKMLEM KKKLGCQSLA
     VGHLSRELEE EREMVRFFRT LGGARKKKV