ID   CAS2_METTH              Reviewed;          91 AA.
AC   O27155;
DT   18-APR-2012, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   03-AUG-2022, entry version 93.
DE   RecName: Full=CRISPR-associated endoribonuclease Cas2;
DE            EC=3.1.-.-;
GN   Name=cas2; OrderedLocusNames=MTH_1083;
OS   Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM
OS   10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum).
OC   Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
OC   Methanobacteriales; Methanobacteriaceae; Methanothermobacter.
OX   NCBI_TaxID=187420;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H;
RX   PubMed=9371463; DOI=10.1128/jb.179.22.7135-7155.1997;
RA   Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J.,
RA   Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D.,
RA   Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R.,
RA   Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D.,
RA   Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A.,
RA   Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J.,
RA   Reeve J.N.;
RT   "Complete genome sequence of Methanobacterium thermoautotrophicum deltaH:
RT   functional analysis and comparative genomics.";
RL   J. Bacteriol. 179:7135-7155(1997).
RN   [2]
RP   FUNCTION AS A SSRNA-SPECIFIC ENDORIBONUCLEASE.
RC   STRAIN=ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H;
RX   PubMed=18482976; DOI=10.1074/jbc.m803225200;
RA   Beloglazova N., Brown G., Zimmerman M.D., Proudfoot M., Makarova K.S.,
RA   Kudritska M., Kochinyan S., Wang S., Chruszcz M., Minor W., Koonin E.V.,
RA   Edwards A.M., Savchenko A., Yakunin A.F.;
RT   "A novel family of sequence-specific endoribonucleases associated with the
RT   clustered regularly interspaced short palindromic repeats.";
RL   J. Biol. Chem. 283:20361-20371(2008).
CC   -!- FUNCTION: CRISPR (clustered regularly interspaced short palindromic
CC       repeat), is an adaptive immune system that provides protection against
CC       mobile genetic elements (viruses, transposable elements and conjugative
CC       plasmids). CRISPR clusters contain sequences complementary to
CC       antecedent mobile elements and target invading nucleic acids. CRISPR
CC       clusters are transcribed and processed into CRISPR RNA (crRNA).
CC       Involved in the integration of spacer DNA into the CRISPR cassette (By
CC       similarity). Functions as a ssRNA-specific endoribonuclease.
CC       {ECO:0000250, ECO:0000269|PubMed:18482976}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC   -!- SUBUNIT: Homodimer, forms a heterotetramer with a Cas1 homodimer.
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the CRISPR-associated endoribonuclease Cas2
CC       protein family. {ECO:0000305}.
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DR   EMBL; AE000666; AAB85572.1; -; Genomic_DNA.
DR   PIR; H69010; H69010.
DR   AlphaFoldDB; O27155; -.
DR   SMR; O27155; -.
DR   STRING; 187420.MTH_1083; -.
DR   EnsemblBacteria; AAB85572; AAB85572; MTH_1083.
DR   KEGG; mth:MTH_1083; -.
DR   PATRIC; fig|187420.15.peg.1061; -.
DR   HOGENOM; CLU_161124_0_1_2; -.
DR   OMA; LTHVQNS; -.
DR   Proteomes; UP000005223; Chromosome.
DR   GO; GO:0004521; F:endoribonuclease activity; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-UniRule.
DR   GO; GO:0043571; P:maintenance of CRISPR repeat elements; IEA:UniProtKB-UniRule.
DR   CDD; cd09725; Cas2_I_II_III; 1.
DR   HAMAP; MF_01471; Cas2; 1.
DR   InterPro; IPR021127; CRISPR_associated_Cas2.
DR   InterPro; IPR019199; Virulence_VapD/CRISPR_Cas2.
DR   PANTHER; PTHR34405; PTHR34405; 1.
DR   Pfam; PF09827; CRISPR_Cas2; 1.
DR   TIGRFAMs; TIGR01573; cas2; 1.
PE   1: Evidence at protein level;
KW   Antiviral defense; Endonuclease; Hydrolase; Magnesium; Metal-binding;
KW   Nuclease; Reference proteome.
FT   CHAIN           1..91
FT                   /note="CRISPR-associated endoribonuclease Cas2"
FT                   /id="PRO_0000416950"
FT   BINDING         12
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   91 AA;  10802 MW;  C79A6E2D987FBF23 CRC64;
     MVVTVYLLIV YDVGVERVNR VKSYLRTELH WVQNSVFEGE VTESQFRRIE TNLERIIDRE
     RDSVIIYSFR SERAMNRNVL GLEKSPLDVI L