ID   CAS2_MYXXD              Reviewed;          97 AA.
AC   Q1CW51;
DT   11-JUL-2012, integrated into UniProtKB/Swiss-Prot.
DT   11-JUL-2006, sequence version 1.
DT   03-AUG-2022, entry version 95.
DE   RecName: Full=CRISPR-associated endoribonuclease Cas2;
DE            EC=3.1.-.-;
GN   Name=cas2; OrderedLocusNames=MXAN_7259;
OS   Myxococcus xanthus (strain DK1622).
OC   Bacteria; Proteobacteria; Deltaproteobacteria; Myxococcales;
OC   Cystobacterineae; Myxococcaceae; Myxococcus.
OX   NCBI_TaxID=246197;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DK1622;
RX   PubMed=17015832; DOI=10.1073/pnas.0607335103;
RA   Goldman B.S., Nierman W.C., Kaiser D., Slater S.C., Durkin A.S., Eisen J.,
RA   Ronning C.M., Barbazuk W.B., Blanchard M., Field C., Halling C., Hinkle G.,
RA   Iartchuk O., Kim H.S., Mackenzie C., Madupu R., Miller N., Shvartsbeyn A.,
RA   Sullivan S.A., Vaudin M., Wiegand R., Kaplan H.B.;
RT   "Evolution of sensory complexity recorded in a myxobacterial genome.";
RL   Proc. Natl. Acad. Sci. U.S.A. 103:15200-15205(2006).
RN   [2]
RP   INDUCTION, AND OPERON STRUCTURE.
RC   STRAIN=DK1622;
RX   PubMed=17369305; DOI=10.1128/jb.00187-07;
RA   Viswanathan P., Murphy K., Julien B., Garza A.G., Kroos L.;
RT   "Regulation of dev, an operon that includes genes essential for Myxococcus
RT   xanthus development and CRISPR-associated genes and repeats.";
RL   J. Bacteriol. 189:3738-3750(2007).
CC   -!- FUNCTION: CRISPR (clustered regularly interspaced short palindromic
CC       repeat), is an adaptive immune system that provides protection against
CC       mobile genetic elements (viruses, transposable elements and conjugative
CC       plasmids). CRISPR clusters contain sequences complementary to
CC       antecedent mobile elements and target invading nucleic acids. CRISPR
CC       clusters are transcribed and processed into CRISPR RNA (crRNA).
CC       Functions as a ssRNA-specific endoribonuclease. Involved in the
CC       integration of spacer DNA into the CRISPR cassette (By similarity).
CC       {ECO:0000250}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC   -!- SUBUNIT: Homodimer, forms a heterotetramer with a Cas1 homodimer.
CC       {ECO:0000250}.
CC   -!- DEVELOPMENTAL STAGE: Operon expression begins by 6 hours after
CC       starvation has initiated development and is under strong negative
CC       regulation by DevS.
CC   -!- INDUCTION: Part of an operon going from at least MXAN_7266 to MXAN_7259
CC       that includes a CRISPR operon with transcription continuing into the
CC       pre-crRNA locus. {ECO:0000269|PubMed:17369305}.
CC   -!- SIMILARITY: Belongs to the CRISPR-associated endoribonuclease Cas2
CC       protein family. {ECO:0000305}.
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DR   EMBL; CP000113; ABF89361.1; -; Genomic_DNA.
DR   RefSeq; WP_011557178.1; NC_008095.1.
DR   AlphaFoldDB; Q1CW51; -.
DR   SMR; Q1CW51; -.
DR   STRING; 246197.MXAN_7259; -.
DR   EnsemblBacteria; ABF89361; ABF89361; MXAN_7259.
DR   GeneID; 41364425; -.
DR   KEGG; mxa:MXAN_7259; -.
DR   eggNOG; COG1343; Bacteria.
DR   HOGENOM; CLU_161124_3_0_7; -.
DR   OMA; TYDIEND; -.
DR   OrthoDB; 1951170at2; -.
DR   Proteomes; UP000002402; Chromosome.
DR   GO; GO:0004521; F:endoribonuclease activity; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-UniRule.
DR   GO; GO:0043571; P:maintenance of CRISPR repeat elements; IEA:UniProtKB-UniRule.
DR   CDD; cd09725; Cas2_I_II_III; 1.
DR   HAMAP; MF_01471; Cas2; 1.
DR   InterPro; IPR021127; CRISPR_associated_Cas2.
DR   InterPro; IPR019199; Virulence_VapD/CRISPR_Cas2.
DR   PANTHER; PTHR34405; PTHR34405; 1.
DR   Pfam; PF09827; CRISPR_Cas2; 1.
DR   TIGRFAMs; TIGR01573; cas2; 1.
PE   2: Evidence at transcript level;
KW   Antiviral defense; Endonuclease; Hydrolase; Magnesium; Metal-binding;
KW   Nuclease; Reference proteome.
FT   CHAIN           1..97
FT                   /note="CRISPR-associated endoribonuclease Cas2"
FT                   /id="PRO_0000418220"
FT   BINDING         13
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   97 AA;  11589 MW;  B514E9C92BC297AA CRC64;
     MAEPRRWYLI TYDIRDPKRW RKVHALLKGY GEWLQLSVFR CSLTDRDREK LRWELSRRMD
     AVDTLLVIGL CGGCVERVRA INAKEDWPEE PAPFKVL