ID   CAS2_NEIM8              Reviewed;         108 AA.
AC   C9X1G7;
DT   03-SEP-2014, integrated into UniProtKB/Swiss-Prot.
DT   24-NOV-2009, sequence version 1.
DT   03-AUG-2022, entry version 57.
DE   RecName: Full=CRISPR-associated endoribonuclease Cas2 {ECO:0000255|HAMAP-Rule:MF_01471};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_01471};
GN   Name=cas2 {ECO:0000255|HAMAP-Rule:MF_01471}; OrderedLocusNames=NMV_1995;
OS   Neisseria meningitidis serogroup C (strain 8013).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Neisseriales; Neisseriaceae;
OC   Neisseria.
OX   NCBI_TaxID=604162;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=8013;
RX   PubMed=19818133; DOI=10.1186/gb-2009-10-10-r110;
RA   Rusniok C., Vallenet D., Floquet S., Ewles H., Mouze-Soulama C., Brown D.,
RA   Lajus A., Buchrieser C., Medigue C., Glaser P., Pelicic V.;
RT   "NeMeSys: a biological resource for narrowing the gap between sequence and
RT   function in the human pathogen Neisseria meningitidis.";
RL   Genome Biol. 10:R110.1-R110.13(2009).
RN   [2]
RP   DISRUPTION PHENOTYPE.
RC   STRAIN=8013;
RX   PubMed=23706818; DOI=10.1016/j.molcel.2013.05.001;
RA   Zhang Y., Heidrich N., Ampattu B.J., Gunderson C.W., Seifert H.S.,
RA   Schoen C., Vogel J., Sontheimer E.J.;
RT   "Processing-independent CRISPR RNAs limit natural transformation in
RT   Neisseria meningitidis.";
RL   Mol. Cell 50:488-503(2013).
CC   -!- FUNCTION: CRISPR (clustered regularly interspaced short palindromic
CC       repeat), is an adaptive immune system that provides protection against
CC       mobile genetic elements (viruses, transposable elements and conjugative
CC       plasmids). CRISPR clusters contain sequences complementary to
CC       antecedent mobile elements and target invading nucleic acids. CRISPR
CC       clusters are transcribed and processed into CRISPR RNA (crRNA).
CC       Functions as a ssRNA-specific endoribonuclease. Involved in the
CC       integration of spacer DNA into the CRISPR cassette. {ECO:0000255|HAMAP-
CC       Rule:MF_01471}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01471};
CC   -!- SUBUNIT: Homodimer, forms a heterotetramer with a Cas1 homodimer.
CC       {ECO:0000255|HAMAP-Rule:MF_01471}.
CC   -!- DISRUPTION PHENOTYPE: No effect on CRISPR interference during plasmid
CC       transformation. {ECO:0000269|PubMed:23706818}.
CC   -!- SIMILARITY: Belongs to the CRISPR-associated endoribonuclease Cas2
CC       protein family. {ECO:0000255|HAMAP-Rule:MF_01471}.
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DR   EMBL; FM999788; CAX50781.1; -; Genomic_DNA.
DR   RefSeq; WP_002214566.1; NC_017501.1.
DR   AlphaFoldDB; C9X1G7; -.
DR   SMR; C9X1G7; -.
DR   GeneID; 61280674; -.
DR   KEGG; nmt:NMV_1995; -.
DR   PATRIC; fig|604162.3.peg.2307; -.
DR   HOGENOM; CLU_150500_1_0_4; -.
DR   OMA; TDKQFGM; -.
DR   Proteomes; UP000002076; Chromosome.
DR   GO; GO:0004521; F:endoribonuclease activity; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-UniRule.
DR   GO; GO:0043571; P:maintenance of CRISPR repeat elements; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_01471; Cas2; 1.
DR   InterPro; IPR021127; CRISPR_associated_Cas2.
DR   InterPro; IPR019199; Virulence_VapD/CRISPR_Cas2.
DR   Pfam; PF09827; CRISPR_Cas2; 1.
DR   TIGRFAMs; TIGR01573; cas2; 1.
PE   3: Inferred from homology;
KW   Antiviral defense; Endonuclease; Hydrolase; Magnesium; Metal-binding;
KW   Nuclease.
FT   CHAIN           1..108
FT                   /note="CRISPR-associated endoribonuclease Cas2"
FT                   /id="PRO_0000429992"
FT   BINDING         14
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01471"
SQ   SEQUENCE   108 AA;  12546 MW;  2CA2643845405FBE CRC64;
     MSEAKFMRII VFFDLPVITA AKRKAANQFR QFLLKDGYQM LQLSVYSRIV KGRDSLQKHH
     NRLCANLPQE GSIRCLEITE KQYAAMKLLL GELKTQEKKV NSDQLLLF