ID   Y4II_SINFN              Reviewed;         703 AA.
AC   P55492;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   25-MAY-2022, entry version 117.
DE   RecName: Full=Uncharacterized protein y4iI;
GN   OrderedLocusNames=NGR_a03240; ORFNames=y4iI;
OS   Sinorhizobium fredii (strain NBRC 101917 / NGR234).
OG   Plasmid sym pNGR234a.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX   NCBI_TaxID=394;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NBRC 101917 / NGR234;
RX   PubMed=9163424; DOI=10.1038/387394a0;
RA   Freiberg C.A., Fellay R., Bairoch A., Broughton W.J., Rosenthal A.,
RA   Perret X.;
RT   "Molecular basis of symbiosis between Rhizobium and legumes.";
RL   Nature 387:394-401(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NBRC 101917 / NGR234;
RX   PubMed=19376903; DOI=10.1128/aem.00515-09;
RA   Schmeisser C., Liesegang H., Krysciak D., Bakkou N., Le Quere A.,
RA   Wollherr A., Heinemeyer I., Morgenstern B., Pommerening-Roeser A.,
RA   Flores M., Palacios R., Brenner S., Gottschalk G., Schmitz R.A.,
RA   Broughton W.J., Perret X., Strittmatter A.W., Streit W.R.;
RT   "Rhizobium sp. strain NGR234 possesses a remarkable number of secretion
RT   systems.";
RL   Appl. Environ. Microbiol. 75:4035-4045(2009).
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
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DR   EMBL; U00090; AAB91704.1; -; Genomic_DNA.
DR   RefSeq; NP_443902.1; NC_000914.2.
DR   RefSeq; WP_010875340.1; NC_000914.2.
DR   AlphaFoldDB; P55492; -.
DR   STRING; 394.NGR_a03240; -.
DR   PRIDE; P55492; -.
DR   EnsemblBacteria; AAB91704; AAB91704; NGR_a03240.
DR   KEGG; rhi:NGR_a03240; -.
DR   PATRIC; fig|394.7.peg.334; -.
DR   eggNOG; COG1752; Bacteria.
DR   HOGENOM; CLU_021032_0_0_5; -.
DR   OMA; DRAVIMI; -.
DR   OrthoDB; 576379at2; -.
DR   Proteomes; UP000001054; Plasmid sym pNGR234a.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016042; P:lipid catabolic process; IEA:UniProtKB-KW.
DR   InterPro; IPR016035; Acyl_Trfase/lysoPLipase.
DR   InterPro; IPR002641; PNPLA_dom.
DR   Pfam; PF01734; Patatin; 1.
DR   SUPFAM; SSF52151; SSF52151; 1.
DR   PROSITE; PS51635; PNPLA; 1.
PE   4: Predicted;
KW   Cell membrane; Hydrolase; Lipid degradation; Lipid metabolism; Membrane;
KW   Plasmid; Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..703
FT                   /note="Uncharacterized protein y4iI"
FT                   /id="PRO_0000200861"
FT   TRANSMEM        23..43
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        69..89
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        143..163
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        250..270
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        357..377
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        432..452
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        644..664
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          23..335
FT                   /note="PNPLA"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01161"
FT   MOTIF           72..76
FT                   /note="GXSXG"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01161"
FT   MOTIF           322..324
FT                   /note="DGA/G"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01161"
FT   ACT_SITE        74
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01161"
FT   ACT_SITE        322
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01161"
SQ   SEQUENCE   703 AA;  76184 MW;  399E4B020C160824 CRC64;
     MRRRSTEGTV MTDGSKPTFE IGIAMSGAIS AGAYSAGVFD FLIQALDAWE KAKAEGAPDL
     PEYDVRLKAL SGASAGAITA AIGVIAAGGR EAPATFPSPA PGSQNIRFTL GRLYRSWVTS
     PTLVSPDGSP DLLSLEDLAG GRPVISVLNA NVLTAIGAEA LEATGTLSPR AYVASSLHLY
     MMLSNLRGVP YAIHFNGGQY NMMTHADRVH YVVEGIGTWK PTPSPFADTD SGTSIAATSL
     FGAAGASTRP PEWLAFANAA LASGAFPIGL SPRVIATATS QYAKAKFPIS EDQSELRPIP
     TWPDAWHVSA SQDYPFSFVS VDGGLINNDP FEFVRFTLMK DPPRPNERNA EKADRAVIMI
     APFPEGPPFL GDGEPPLGVL SIARRVVTAL RQQVRFKPDQ LLAVAAEGTH SRFMISPHRV
     PPSTPGGEER EETFSIASGL LGGFGGFVLE AFRDHDYQLG RRNCQYFLMR HLTIDKNHQT
     LHWPEGAAER RNAVISKTLS DGSMHDYVPI IPLVGDALPE VPYPRWARID ENAFALLVKR
     IEARLVAVAR RLVSTETTSA RMKLGLNFLL LVGRNRIVDY IRLTLLQELV MRDQIEGWPL
     PAADLRPDFV RAVLAALLDP AFDLRTEAGI ARTTKLDTSL VREILSTLAG AAGANCQVWL
     APWTRTDEPS LYTLVSRRPS FLATLLQGRS PARLFAKPVV DRK