Y4JK_SINFN
ID Y4JK_SINFN Reviewed; 140 AA.
AC P55511;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 1.
DT 03-AUG-2022, entry version 115.
DE RecName: Full=VapC ribonuclease Y4jK;
DE Short=RNase Y4jK;
DE EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_00265};
DE AltName: Full=Putative plasmid stability protein Y4jK;
DE AltName: Full=Toxin Y4jK;
GN OrderedLocusNames=NGR_a03040; ORFNames=y4jK;
OS Sinorhizobium fredii (strain NBRC 101917 / NGR234).
OG Plasmid sym pNGR234a.
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX NCBI_TaxID=394;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NBRC 101917 / NGR234;
RX PubMed=9163424; DOI=10.1038/387394a0;
RA Freiberg C.A., Fellay R., Bairoch A., Broughton W.J., Rosenthal A.,
RA Perret X.;
RT "Molecular basis of symbiosis between Rhizobium and legumes.";
RL Nature 387:394-401(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NBRC 101917 / NGR234;
RX PubMed=19376903; DOI=10.1128/aem.00515-09;
RA Schmeisser C., Liesegang H., Krysciak D., Bakkou N., Le Quere A.,
RA Wollherr A., Heinemeyer I., Morgenstern B., Pommerening-Roeser A.,
RA Flores M., Palacios R., Brenner S., Gottschalk G., Schmitz R.A.,
RA Broughton W.J., Perret X., Strittmatter A.W., Streit W.R.;
RT "Rhizobium sp. strain NGR234 possesses a remarkable number of secretion
RT systems.";
RL Appl. Environ. Microbiol. 75:4035-4045(2009).
CC -!- FUNCTION: Toxic component of a type II toxin-antitoxin (TA) system. An
CC RNase (By similarity). Involved in plasmid stability (Probable).
CC {ECO:0000255|HAMAP-Rule:MF_00265, ECO:0000305}.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00265};
CC -!- SIMILARITY: Belongs to the PINc/VapC protein family.
CC {ECO:0000255|HAMAP-Rule:MF_00265}.
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DR EMBL; U00090; AAB91723.1; -; Genomic_DNA.
DR PIR; T28638; T28638.
DR RefSeq; NP_443921.1; NC_000914.2.
DR RefSeq; WP_010875325.1; NC_000914.2.
DR AlphaFoldDB; P55511; -.
DR SMR; P55511; -.
DR STRING; 394.NGR_a03040; -.
DR EnsemblBacteria; AAB91723; AAB91723; NGR_a03040.
DR KEGG; rhi:NGR_a03040; -.
DR PATRIC; fig|394.7.peg.316; -.
DR eggNOG; COG1487; Bacteria.
DR HOGENOM; CLU_118482_8_2_5; -.
DR OMA; MQIAGIC; -.
DR OrthoDB; 1968541at2; -.
DR Proteomes; UP000001054; Plasmid sym pNGR234a.
DR GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004540; F:ribonuclease activity; IEA:InterPro.
DR HAMAP; MF_00265; VapC_Nob1; 1.
DR InterPro; IPR029060; PIN-like_dom_sf.
DR InterPro; IPR002716; PIN_dom.
DR InterPro; IPR022907; VapC_family.
DR Pfam; PF01850; PIN; 1.
DR SUPFAM; SSF88723; SSF88723; 1.
PE 3: Inferred from homology;
KW Hydrolase; Magnesium; Metal-binding; Nuclease; Plasmid; Reference proteome;
KW Toxin-antitoxin system.
FT CHAIN 1..140
FT /note="VapC ribonuclease Y4jK"
FT /id="PRO_0000200877"
FT DOMAIN 2..135
FT /note="PINc"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00265"
FT BINDING 5
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00265"
FT BINDING 104
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00265"
SQ SEQUENCE 140 AA; 15119 MW; 9142E3F8C2BFCD0C CRC64;
MIVLDTNVIS ELWKAEPDRA VLAWIDAQMI ETLYLSAITV AELRFGLAAM PAGKRRTIFQ
NRLEGEVLPA LAGRVLPFDL DASRSYADLM AQAKTSGKVI GKADGYIAAT AVAHGFMVAT
RDTSPFEAAG LDIINPWEPA
