ID   CAS2_THEMA              Reviewed;          87 AA.
AC   Q9X2B6;
DT   18-APR-2012, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1999, sequence version 1.
DT   03-AUG-2022, entry version 99.
DE   RecName: Full=CRISPR-associated endoribonuclease Cas2;
DE            EC=3.1.-.-;
GN   Name=cas2; OrderedLocusNames=TM_1796;
OS   Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826
OS   / MSB8).
OC   Bacteria; Thermotogae; Thermotogales; Thermotogaceae; Thermotoga.
OX   NCBI_TaxID=243274;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8;
RX   PubMed=10360571; DOI=10.1038/20601;
RA   Nelson K.E., Clayton R.A., Gill S.R., Gwinn M.L., Dodson R.J., Haft D.H.,
RA   Hickey E.K., Peterson J.D., Nelson W.C., Ketchum K.A., McDonald L.A.,
RA   Utterback T.R., Malek J.A., Linher K.D., Garrett M.M., Stewart A.M.,
RA   Cotton M.D., Pratt M.S., Phillips C.A., Richardson D.L., Heidelberg J.F.,
RA   Sutton G.G., Fleischmann R.D., Eisen J.A., White O., Salzberg S.L.,
RA   Smith H.O., Venter J.C., Fraser C.M.;
RT   "Evidence for lateral gene transfer between Archaea and Bacteria from
RT   genome sequence of Thermotoga maritima.";
RL   Nature 399:323-329(1999).
RN   [2]
RP   FUNCTION AS A SSRNA-SPECIFIC ENDORIBONUCLEASE.
RC   STRAIN=ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8;
RX   PubMed=18482976; DOI=10.1074/jbc.m803225200;
RA   Beloglazova N., Brown G., Zimmerman M.D., Proudfoot M., Makarova K.S.,
RA   Kudritska M., Kochinyan S., Wang S., Chruszcz M., Minor W., Koonin E.V.,
RA   Edwards A.M., Savchenko A., Yakunin A.F.;
RT   "A novel family of sequence-specific endoribonucleases associated with the
RT   clustered regularly interspaced short palindromic repeats.";
RL   J. Biol. Chem. 283:20361-20371(2008).
CC   -!- FUNCTION: CRISPR (clustered regularly interspaced short palindromic
CC       repeat), is an adaptive immune system that provides protection against
CC       mobile genetic elements (viruses, transposable elements and conjugative
CC       plasmids). CRISPR clusters contain sequences complementary to
CC       antecedent mobile elements and target invading nucleic acids. CRISPR
CC       clusters are transcribed and processed into CRISPR RNA (crRNA).
CC       Involved in the integration of spacer DNA into the CRISPR cassette (By
CC       similarity). Functions as a ssRNA-specific endoribonuclease.
CC       {ECO:0000250, ECO:0000269|PubMed:18482976}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC   -!- SUBUNIT: Homodimer, forms a heterotetramer with a Cas1 homodimer.
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the CRISPR-associated endoribonuclease Cas2
CC       protein family. {ECO:0000305}.
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DR   EMBL; AE000512; AAD36859.1; -; Genomic_DNA.
DR   PIR; A72210; A72210.
DR   RefSeq; NP_229593.1; NC_000853.1.
DR   RefSeq; WP_004082343.1; NZ_CP011107.1.
DR   AlphaFoldDB; Q9X2B6; -.
DR   SMR; Q9X2B6; -.
DR   STRING; 243274.THEMA_05210; -.
DR   EnsemblBacteria; AAD36859; AAD36859; TM_1796.
DR   KEGG; tma:TM1796; -.
DR   eggNOG; COG1343; Bacteria.
DR   InParanoid; Q9X2B6; -.
DR   OMA; LTHVQNS; -.
DR   OrthoDB; 1951170at2; -.
DR   Proteomes; UP000008183; Chromosome.
DR   GO; GO:0004521; F:endoribonuclease activity; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-UniRule.
DR   GO; GO:0043571; P:maintenance of CRISPR repeat elements; IEA:UniProtKB-UniRule.
DR   CDD; cd09725; Cas2_I_II_III; 1.
DR   HAMAP; MF_01471; Cas2; 1.
DR   InterPro; IPR021127; CRISPR_associated_Cas2.
DR   InterPro; IPR019199; Virulence_VapD/CRISPR_Cas2.
DR   PANTHER; PTHR34405; PTHR34405; 1.
DR   Pfam; PF09827; CRISPR_Cas2; 1.
DR   TIGRFAMs; TIGR01573; cas2; 1.
PE   1: Evidence at protein level;
KW   Antiviral defense; Endonuclease; Hydrolase; Magnesium; Metal-binding;
KW   Nuclease; Reference proteome.
FT   CHAIN           1..87
FT                   /note="CRISPR-associated endoribonuclease Cas2"
FT                   /id="PRO_0000416947"
FT   BINDING         8
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   87 AA;  10238 MW;  393705D9485F556B CRC64;
     MYVIMVYDVN EKRVAKILKI ARKYLKWVQN SVLEGELSPG KYEKLKLEVS RLIDEKEDSV
     RFYVMDSQKV FNLETLGVEK GEDGFIF