CAS2_TREDE
ID CAS2_TREDE Reviewed; 101 AA.
AC Q73QW4;
DT 13-JUN-2012, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 82.
DE RecName: Full=CRISPR-associated endoribonuclease Cas2 {ECO:0000255|HAMAP-Rule:MF_01471};
DE EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_01471};
GN Name=cas2 {ECO:0000255|HAMAP-Rule:MF_01471}; OrderedLocusNames=TDE_0329;
OS Treponema denticola (strain ATCC 35405 / DSM 14222 / CIP 103919 / JCM 8153
OS / KCTC 15104).
OC Bacteria; Spirochaetes; Spirochaetales; Treponemataceae; Treponema.
OX NCBI_TaxID=243275;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 35405 / DSM 14222 / CIP 103919 / JCM 8153 / KCTC 15104;
RX PubMed=15064399; DOI=10.1073/pnas.0307639101;
RA Seshadri R., Myers G.S.A., Tettelin H., Eisen J.A., Heidelberg J.F.,
RA Dodson R.J., Davidsen T.M., DeBoy R.T., Fouts D.E., Haft D.H., Selengut J.,
RA Ren Q., Brinkac L.M., Madupu R., Kolonay J.F., Durkin S.A., Daugherty S.C.,
RA Shetty J., Shvartsbeyn A., Gebregeorgis E., Geer K., Tsegaye G.,
RA Malek J.A., Ayodeji B., Shatsman S., McLeod M.P., Smajs D., Howell J.K.,
RA Pal S., Amin A., Vashisth P., McNeill T.Z., Xiang Q., Sodergren E.,
RA Baca E., Weinstock G.M., Norris S.J., Fraser C.M., Paulsen I.T.;
RT "Comparison of the genome of the oral pathogen Treponema denticola with
RT other spirochete genomes.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:5646-5651(2004).
CC -!- FUNCTION: CRISPR (clustered regularly interspaced short palindromic
CC repeat), is an adaptive immune system that provides protection against
CC mobile genetic elements (viruses, transposable elements and conjugative
CC plasmids). CRISPR clusters contain sequences complementary to
CC antecedent mobile elements and target invading nucleic acids. CRISPR
CC clusters are transcribed and processed into CRISPR RNA (crRNA).
CC Functions as a ssRNA-specific endoribonuclease. Involved in the
CC integration of spacer DNA into the CRISPR cassette. {ECO:0000255|HAMAP-
CC Rule:MF_01471}.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01471};
CC -!- SUBUNIT: Homodimer, forms a heterotetramer with a Cas1 homodimer.
CC {ECO:0000255|HAMAP-Rule:MF_01471}.
CC -!- SIMILARITY: Belongs to the CRISPR-associated endoribonuclease Cas2
CC protein family. {ECO:0000255|HAMAP-Rule:MF_01471}.
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DR EMBL; AE017226; AAS10824.1; -; Genomic_DNA.
DR RefSeq; NP_970943.1; NC_002967.9.
DR RefSeq; WP_002666293.1; NC_002967.9.
DR PDB; 6JHZ; X-ray; 2.00 A; A/B=1-93.
DR PDBsum; 6JHZ; -.
DR AlphaFoldDB; Q73QW4; -.
DR SMR; Q73QW4; -.
DR STRING; 243275.TDE_0329; -.
DR DNASU; 2741545; -.
DR EnsemblBacteria; AAS10824; AAS10824; TDE_0329.
DR GeneID; 2741545; -.
DR KEGG; tde:TDE_0329; -.
DR PATRIC; fig|243275.7.peg.318; -.
DR eggNOG; COG3512; Bacteria.
DR HOGENOM; CLU_150500_1_1_12; -.
DR OrthoDB; 1841250at2; -.
DR Proteomes; UP000008212; Chromosome.
DR GO; GO:0004521; F:endoribonuclease activity; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-UniRule.
DR GO; GO:0043571; P:maintenance of CRISPR repeat elements; IEA:UniProtKB-UniRule.
DR HAMAP; MF_01471; Cas2; 1.
DR InterPro; IPR021127; CRISPR_associated_Cas2.
DR InterPro; IPR019199; Virulence_VapD/CRISPR_Cas2.
DR Pfam; PF09827; CRISPR_Cas2; 1.
DR TIGRFAMs; TIGR01573; cas2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Antiviral defense; Endonuclease; Hydrolase; Magnesium;
KW Metal-binding; Nuclease; Reference proteome.
FT CHAIN 1..101
FT /note="CRISPR-associated endoribonuclease Cas2"
FT /id="PRO_0000417739"
FT BINDING 8
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01471"
FT STRAND 2..8
FT /evidence="ECO:0007829|PDB:6JHZ"
FT HELIX 14..29
FT /evidence="ECO:0007829|PDB:6JHZ"
FT STRAND 33..36
FT /evidence="ECO:0007829|PDB:6JHZ"
FT STRAND 39..43
FT /evidence="ECO:0007829|PDB:6JHZ"
FT HELIX 47..59
FT /evidence="ECO:0007829|PDB:6JHZ"
FT STRAND 63..72
FT /evidence="ECO:0007829|PDB:6JHZ"
FT HELIX 74..78
FT /evidence="ECO:0007829|PDB:6JHZ"
FT STRAND 81..84
FT /evidence="ECO:0007829|PDB:6JHZ"
SQ SEQUENCE 101 AA; 11779 MW; E3E0BEAF583703C8 CRC64;
MRVIVFFDLP VITPENRHNY SVFRKYLIKS GFIMQQKSVY SKLVLNLTNR DSIVKSIEKN
KPPEGLVEVL TVTEKQYAKM EIIIGESKTE YLNTDERLVV L
