ID   Y4SG_SINFN              Reviewed;         336 AA.
AC   P55650;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   25-MAY-2022, entry version 117.
DE   RecName: Full=Uncharacterized protein y4sG;
GN   OrderedLocusNames=NGR_a01660; ORFNames=y4sG;
OS   Sinorhizobium fredii (strain NBRC 101917 / NGR234).
OG   Plasmid sym pNGR234a.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX   NCBI_TaxID=394;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NBRC 101917 / NGR234;
RX   PubMed=9163424; DOI=10.1038/387394a0;
RA   Freiberg C.A., Fellay R., Bairoch A., Broughton W.J., Rosenthal A.,
RA   Perret X.;
RT   "Molecular basis of symbiosis between Rhizobium and legumes.";
RL   Nature 387:394-401(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NBRC 101917 / NGR234;
RX   PubMed=19376903; DOI=10.1128/aem.00515-09;
RA   Schmeisser C., Liesegang H., Krysciak D., Bakkou N., Le Quere A.,
RA   Wollherr A., Heinemeyer I., Morgenstern B., Pommerening-Roeser A.,
RA   Flores M., Palacios R., Brenner S., Gottschalk G., Schmitz R.A.,
RA   Broughton W.J., Perret X., Strittmatter A.W., Streit W.R.;
RT   "Rhizobium sp. strain NGR234 possesses a remarkable number of secretion
RT   systems.";
RL   Appl. Environ. Microbiol. 75:4035-4045(2009).
CC   -!- FUNCTION: Could be involved in the biosynthesis of a cell wall
CC       component.
CC   -!- SIMILARITY: Belongs to the D-alanine--D-alanine ligase family.
CC       {ECO:0000305}.
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DR   EMBL; U00090; AAB91846.1; -; Genomic_DNA.
DR   RefSeq; NP_444059.1; NC_000914.2.
DR   RefSeq; WP_010875202.1; NC_000914.2.
DR   AlphaFoldDB; P55650; -.
DR   SMR; P55650; -.
DR   STRING; 394.NGR_a01660; -.
DR   EnsemblBacteria; AAB91846; AAB91846; NGR_a01660.
DR   KEGG; rhi:NGR_a01660; -.
DR   eggNOG; COG1181; Bacteria.
DR   HOGENOM; CLU_039268_2_0_5; -.
DR   OMA; DINPNCD; -.
DR   OrthoDB; 764798at2; -.
DR   Proteomes; UP000001054; Plasmid sym pNGR234a.
DR   GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR   GO; GO:0008716; F:D-alanine-D-alanine ligase activity; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR   GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.1490.20; -; 1.
DR   InterPro; IPR011761; ATP-grasp.
DR   InterPro; IPR013815; ATP_grasp_subdomain_1.
DR   InterPro; IPR011095; Dala_Dala_lig_C.
DR   InterPro; IPR016185; PreATP-grasp_dom_sf.
DR   Pfam; PF07478; Dala_Dala_lig_C; 1.
DR   SUPFAM; SSF52440; SSF52440; 1.
DR   PROSITE; PS50975; ATP_GRASP; 1.
PE   3: Inferred from homology;
KW   Cell wall biogenesis/degradation; Ligase; Plasmid; Reference proteome.
FT   CHAIN           1..336
FT                   /note="Uncharacterized protein y4sG"
FT                   /id="PRO_0000177921"
FT   DOMAIN          123..323
FT                   /note="ATP-grasp"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00409"
SQ   SEQUENCE   336 AA;  36972 MW;  29A8FE133704BCAA CRC64;
     MRIAVVCNSA FTGVINRFGQ PYPQPPQPWP QGRIADSVVA ALQECGHETL LCEGDKGLLT
     TLERFMPPDP LARTSGMVFN LAEGIQGEYR FTHVPAMLEM AGVPYTGSSP LGHGLTDDKV
     ISKTLMRDSG VPTPNFSIMR RGTESTDDLR FPVVVKPRHE DNSFGLQLVH EPAQLQQAVK
     MIVTKYAQDA LVEEYIDGRE IHVALLGNQE VEVLPMVEFE LGEHEAPLLT WEAKYLAAVQ
     PPKTCPAKIE SKLATLLRDI SVATFRACQC RDYARVDLRL DRSGQPFVLE INSMPGLSTH
     SAYVLAAMTA GHSYSSLING ILDVAHRRYF GNGILG