Y4WA_SINFN
ID Y4WA_SINFN Reviewed; 512 AA.
AC P55679;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 1.
DT 03-AUG-2022, entry version 133.
DE RecName: Full=Uncharacterized zinc protease y4wA;
DE EC=3.4.24.-;
GN OrderedLocusNames=NGR_a01040; ORFNames=y4wA;
OS Sinorhizobium fredii (strain NBRC 101917 / NGR234).
OG Plasmid sym pNGR234a.
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium.
OX NCBI_TaxID=394;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NBRC 101917 / NGR234;
RX PubMed=9163424; DOI=10.1038/387394a0;
RA Freiberg C.A., Fellay R., Bairoch A., Broughton W.J., Rosenthal A.,
RA Perret X.;
RT "Molecular basis of symbiosis between Rhizobium and legumes.";
RL Nature 387:394-401(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NBRC 101917 / NGR234;
RX PubMed=19376903; DOI=10.1128/aem.00515-09;
RA Schmeisser C., Liesegang H., Krysciak D., Bakkou N., Le Quere A.,
RA Wollherr A., Heinemeyer I., Morgenstern B., Pommerening-Roeser A.,
RA Flores M., Palacios R., Brenner S., Gottschalk G., Schmitz R.A.,
RA Broughton W.J., Perret X., Strittmatter A.W., Streit W.R.;
RT "Rhizobium sp. strain NGR234 possesses a remarkable number of secretion
RT systems.";
RL Appl. Environ. Microbiol. 75:4035-4045(2009).
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000250};
CC Note=Binds 1 zinc ion per subunit. {ECO:0000250};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Single-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the peptidase M16 family. {ECO:0000305}.
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DR EMBL; U00090; AAB91908.1; -; Genomic_DNA.
DR RefSeq; NP_444121.1; NC_000914.2.
DR RefSeq; WP_010875145.1; NC_000914.2.
DR AlphaFoldDB; P55679; -.
DR SMR; P55679; -.
DR STRING; 394.NGR_a01040; -.
DR MEROPS; M16.019; -.
DR EnsemblBacteria; AAB91908; AAB91908; NGR_a01040.
DR KEGG; rhi:NGR_a01040; -.
DR PATRIC; fig|394.7.peg.89; -.
DR eggNOG; COG0612; Bacteria.
DR HOGENOM; CLU_009902_1_0_5; -.
DR OMA; FFEHLLF; -.
DR OrthoDB; 1188251at2; -.
DR Proteomes; UP000001054; Plasmid sym pNGR234a.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0004222; F:metalloendopeptidase activity; IEA:InterPro.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR InterPro; IPR011249; Metalloenz_LuxS/M16.
DR InterPro; IPR011765; Pept_M16_N.
DR InterPro; IPR001431; Pept_M16_Zn_BS.
DR InterPro; IPR007863; Peptidase_M16_C.
DR Pfam; PF00675; Peptidase_M16; 1.
DR Pfam; PF05193; Peptidase_M16_C; 1.
DR SUPFAM; SSF63411; SSF63411; 2.
DR PROSITE; PS00143; INSULINASE; 1.
PE 3: Inferred from homology;
KW Cell membrane; Hydrolase; Membrane; Metal-binding; Metalloprotease;
KW Plasmid; Protease; Reference proteome; Transmembrane; Transmembrane helix;
KW Zinc.
FT CHAIN 1..512
FT /note="Uncharacterized zinc protease y4wA"
FT /id="PRO_0000074424"
FT TRANSMEM 57..77
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..50
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 134
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10096"
FT BINDING 131
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10096"
FT BINDING 135
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10096"
FT BINDING 211
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10096"
SQ SEQUENCE 512 AA; 56886 MW; 7BDC60C11F08BD85 CRC64;
MSEPFFRPGS GPAPGHPTAE PWRRRKRWYS PSDGRAGTAR SHLPQRSTSH DGVCSRICAF
VLCMVALQFL MTSAMAADES PLREAEVANF MLGNGMEVVV IPDHRAPIVT QMIWYKVGNA
DEPPGKSGIA HFLEHLMFKG TKKHPSGEFS AKIAEIGGEE NAFTGSDYTA YHQTVTPESL
RTMMEFEADR MRHLVLTDAV IVPERDVILE ERRWRVENDP EQLLEEEMQA TLYQNHPYRI
PTIGWMHEME QLNREDALKF YDRYYAPNNA ILVVAGDVDA GRVRQLADET FGTLPRGPDL
PARVRPQEPE QNTKRIVALT DPRVTVPSFQ KSWVTTSYGT AEQGEAEALD ILSEILGGGT
RSRIYQELVV KQAIASSGGA YFNGRSLDPS SFTVFGSPRG EAKIEEVEDA IDAEIRKIIE
FGITDVELEK AKNRFVRSII FARDSQSGMA GIYGAALATG DTAHDVEAWP LRIRAVKAAE
VQAAARKYLS PDRSVAGYLL PRESATSGDK SR