CAS3_MYXXD
ID CAS3_MYXXD Reviewed; 746 AA.
AC Q1CW46;
DT 11-JUL-2012, integrated into UniProtKB/Swiss-Prot.
DT 11-JUL-2006, sequence version 1.
DT 03-AUG-2022, entry version 101.
DE RecName: Full=Putative CRISPR-associated nuclease/helicase Cas3;
DE EC=3.1.-.-;
DE EC=3.6.4.-;
GN Name=cas3; OrderedLocusNames=MXAN_7264;
OS Myxococcus xanthus (strain DK1622).
OC Bacteria; Proteobacteria; Deltaproteobacteria; Myxococcales;
OC Cystobacterineae; Myxococcaceae; Myxococcus.
OX NCBI_TaxID=246197;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DK1622;
RX PubMed=17015832; DOI=10.1073/pnas.0607335103;
RA Goldman B.S., Nierman W.C., Kaiser D., Slater S.C., Durkin A.S., Eisen J.,
RA Ronning C.M., Barbazuk W.B., Blanchard M., Field C., Halling C., Hinkle G.,
RA Iartchuk O., Kim H.S., Mackenzie C., Madupu R., Miller N., Shvartsbeyn A.,
RA Sullivan S.A., Vaudin M., Wiegand R., Kaplan H.B.;
RT "Evolution of sensory complexity recorded in a myxobacterial genome.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:15200-15205(2006).
RN [2]
RP DEVELOPMENTAL STAGE, INDUCTION, AND OPERON STRUCTURE.
RC STRAIN=DK1622;
RX PubMed=17369305; DOI=10.1128/jb.00187-07;
RA Viswanathan P., Murphy K., Julien B., Garza A.G., Kroos L.;
RT "Regulation of dev, an operon that includes genes essential for Myxococcus
RT xanthus development and CRISPR-associated genes and repeats.";
RL J. Bacteriol. 189:3738-3750(2007).
CC -!- FUNCTION: CRISPR (clustered regularly interspaced short palindromic
CC repeat), is an adaptive immune system that provides protection against
CC mobile genetic elements (viruses, transposable elements and conjugative
CC plasmids). CRISPR clusters contain sequences complementary to
CC antecedent mobile elements and target invading nucleic acids. CRISPR
CC clusters are transcribed and processed into CRISPR RNA (crRNA). This
CC protein plus Cascade participate in CRISPR interference, the third
CC stage of CRISPR immunity. {ECO:0000250|UniProtKB:P38036}.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000250|UniProtKB:Q53VY2};
CC -!- DEVELOPMENTAL STAGE: Operon expression begins by 6 hours after
CC starvation has initiated development and is under strong negative
CC regulation by DevS. {ECO:0000269|PubMed:17369305}.
CC -!- INDUCTION: Part of an operon going from at least MXAN_7266 to MXAN_7259
CC that includes a CRISPR operon with transcription continuing into the
CC pre-crRNA locus. {ECO:0000269|PubMed:17369305}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the CRISPR-associated
CC nuclease Cas3-HD family. {ECO:0000305}.
CC -!- SIMILARITY: In the central section; belongs to the CRISPR-associated
CC helicase Cas3 family. {ECO:0000305}.
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DR EMBL; CP000113; ABF91789.1; -; Genomic_DNA.
DR RefSeq; WP_011557183.1; NC_008095.1.
DR AlphaFoldDB; Q1CW46; -.
DR SMR; Q1CW46; -.
DR STRING; 246197.MXAN_7264; -.
DR EnsemblBacteria; ABF91789; ABF91789; MXAN_7264.
DR GeneID; 41364430; -.
DR KEGG; mxa:MXAN_7264; -.
DR eggNOG; COG1203; Bacteria.
DR HOGENOM; CLU_013108_0_0_7; -.
DR OMA; FCYPTTG; -.
DR OrthoDB; 1149839at2; -.
DR Proteomes; UP000002402; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0004518; F:nuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR Gene3D; 1.10.3210.30; -; 1.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR035011; Cas3.
DR InterPro; IPR006483; CRISPR-assoc_Cas3_HD.
DR InterPro; IPR038257; CRISPR-assoc_Cas3_HD_sf.
DR InterPro; IPR006474; Helicase_Cas3_CRISPR-ass_core.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR47959:SF5; PTHR47959:SF5; 1.
DR Pfam; PF18019; HD_6; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR01587; cas3_core; 1.
DR TIGRFAMs; TIGR01596; cas3_HD; 1.
DR PROSITE; PS51643; HD_CAS3; 1.
PE 2: Evidence at transcript level;
KW Antiviral defense; ATP-binding; Helicase; Hydrolase; Magnesium;
KW Metal-binding; Nuclease; Nucleotide-binding; Reference proteome.
FT CHAIN 1..746
FT /note="Putative CRISPR-associated nuclease/helicase Cas3"
FT /id="PRO_0000418221"
FT DOMAIN 14..230
FT /note="HD Cas3-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00974"
FT MOTIF 412..415
FT /note="DEAH box"
FT BINDING 69
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250|UniProtKB:Q53VY2"
FT BINDING 131
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250|UniProtKB:Q53VY2"
SQ SEQUENCE 746 AA; 82292 MW; 7B1A00D279E4EFFA CRC64;
MKRLLAKSTA TPDRPEGEAT LLGHTALVLS AARRLLEHRG RASLLAAGLD PALEPRLRRI
VLLAAALHDL GKCSEHFQSM LRRQREAPQL VRHEALSLWL CWPGQPLSAW LQRDVSELDL
CLALVCVAAH HRKFQTEAFA PDGTGAGLSL ELRVQHEDFA RTLGRIAEEL ALSAPPLFTA
PIVLRATRKE HPRDQLQSWQ DDFERTVPAG SVDARLLAVC KALVLAADVA GSALPRSGEK
QDWVDRQLTA PHPAEALRAV VERRLAGHTP RPFQEEVARS AAPLTLVRAG CGSGKTAAAY
LWAARQHPGR PLWLTYPTMG TATEGFRDYL HGADVEARLQ HSRAEVDFDI FGLRDGAAPG
TSSRDQDRLD ALRSWGADAM SCTADTVLGL VQSQRQGLYA WPGLCAACVV FDEVHAYDDR
LFGCLLRFLE ALPGIPALLM TASLPATRLD VLRGMCERVH GRSLAEVEGP EDLETLPRYQ
RLDVAEPWAL VAECLRDHGK VLWVSNTVDR CMRTAEAGTS HGARALLYHS RFRYEDRVRR
HGDVIEAFAT EGRAAFASTT QVAEMSLDLS ADLLVTDLAP IPALIQRLGR LNRRSTLERP
APVRPFVVLP FDGPPYAAPD LRDARAWMER LGTGPLSQRD LVDAWGPPGM MDAPRRQSST
WLDGRFDTWP APCRDGSPSL TVLLEEDARA VLDGAVSAHR VTLPMNLPPE SFKWRAWPRA
GRLPYPIPPA NALDYDGLRG ARWRKP
