ID   CAS5A_SACS2             Reviewed;         240 AA.
AC   Q97Y92;
DT   13-JUN-2012, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2001, sequence version 1.
DT   25-MAY-2022, entry version 78.
DE   RecName: Full=CRISPR system aCascade subunit Cas5 1;
GN   Name=cas5a; OrderedLocusNames=SSO1441;
OS   Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
OS   (Sulfolobus solfataricus).
OC   Archaea; Crenarchaeota; Thermoprotei; Sulfolobales; Sulfolobaceae;
OC   Saccharolobus.
OX   NCBI_TaxID=273057;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 35092 / DSM 1617 / JCM 11322 / P2;
RX   PubMed=11427726; DOI=10.1073/pnas.141222098;
RA   She Q., Singh R.K., Confalonieri F., Zivanovic Y., Allard G., Awayez M.J.,
RA   Chan-Weiher C.C.-Y., Clausen I.G., Curtis B.A., De Moors A., Erauso G.,
RA   Fletcher C., Gordon P.M.K., Heikamp-de Jong I., Jeffries A.C., Kozera C.J.,
RA   Medina N., Peng X., Thi-Ngoc H.P., Redder P., Schenk M.E., Theriault C.,
RA   Tolstrup N., Charlebois R.L., Doolittle W.F., Duguet M., Gaasterland T.,
RA   Garrett R.A., Ragan M.A., Sensen C.W., Van der Oost J.;
RT   "The complete genome of the crenarchaeon Sulfolobus solfataricus P2.";
RL   Proc. Natl. Acad. Sci. U.S.A. 98:7835-7840(2001).
RN   [2]
RP   IDENTIFICATION BY MASS SPECTROMETRY, SUBUNIT, AND INTERACTION WITH CSA2.
RC   STRAIN=ATCC 35091 / DSM 1616 / JCM 8930 / NBRC 15331 / P1, and
RC   ATCC 35092 / DSM 1617 / JCM 11322 / P2;
RX   PubMed=21507944; DOI=10.1074/jbc.m111.238485;
RA   Lintner N.G., Kerou M., Brumfield S.K., Graham S., Liu H., Naismith J.H.,
RA   Sdano M., Peng N., She Q., Copie V., Young M.J., White M.F., Lawrence C.M.;
RT   "Structural and functional characterization of an archaeal clustered
RT   regularly interspaced short palindromic repeat (CRISPR)-associated complex
RT   for antiviral defense (CASCADE).";
RL   J. Biol. Chem. 286:21643-21656(2011).
CC   -!- FUNCTION: CRISPR (clustered regularly interspaced short palindromic
CC       repeat) is an adaptive immune system that provides protection against
CC       mobile genetic elements (viruses, transposable elements and conjugative
CC       plasmids). CRISPR clusters contain spacers, sequences complementary to
CC       antecedent mobile elements, and target invading nucleic acids. CRISPR
CC       clusters are transcribed and processed into CRISPR RNA (crRNA) (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Part of the aCascade ribonucleoprotein complex, minimally
CC       composed of Csa2 and Cas5a, which binds crRNA. Other possible
CC       components of aCascade in strain P1 are Cas6b (SSO1437) and Csa5
CC       (SSO1443), while SSO1399, Cas5b (SSO1400) and SSO1401 have sometimes
CC       been seen weakly associated. Csa2 is probably the major RNA-binding
CC       subunit. The Csa2-Cas5a-crRNA complex also binds target DNA homologous
CC       to crRNA, probably forming an R-loop. Purified aCascade forms a
CC       filament about 6 nm in width. {ECO:0000269|PubMed:21507944}.
CC   -!- MISCELLANEOUS: Cloning was done with strain P2, while interaction
CC       experiments were done with genes cloned from strain P2 but over-
CC       expressed in P1. The aCascade complex was purified from strain P1.
CC   -!- SIMILARITY: Belongs to the CRISPR-associated protein Cas5 family.
CC       Subtype I-A/Apern subfamily. {ECO:0000305}.
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DR   EMBL; AE006641; AAK41674.1; -; Genomic_DNA.
DR   PIR; C90302; C90302.
DR   RefSeq; WP_010923406.1; NC_002754.1.
DR   AlphaFoldDB; Q97Y92; -.
DR   SMR; Q97Y92; -.
DR   STRING; 273057.SSO1441; -.
DR   EnsemblBacteria; AAK41674; AAK41674; SSO1441.
DR   GeneID; 27427809; -.
DR   KEGG; sso:SSO1441; -.
DR   PATRIC; fig|273057.12.peg.1470; -.
DR   eggNOG; arCOG02670; Archaea.
DR   HOGENOM; CLU_1154391_0_0_2; -.
DR   InParanoid; Q97Y92; -.
DR   Proteomes; UP000001974; Chromosome.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR   InterPro; IPR013422; CRISPR-assoc_prot_Cas5_N.
DR   InterPro; IPR010153; CRISPR-assoc_prot_Cas5a-typ.
DR   TIGRFAMs; TIGR01874; cas_cas5a; 1.
DR   TIGRFAMs; TIGR02593; CRISPR_cas5; 1.
PE   1: Evidence at protein level;
KW   Antiviral defense; Reference proteome; RNA-binding.
FT   CHAIN           1..240
FT                   /note="CRISPR system aCascade subunit Cas5 1"
FT                   /id="PRO_0000417892"
SQ   SEQUENCE   240 AA;  27397 MW;  6002773E6BD6A2FB CRC64;
     MIYSKVFLKL HWGFSVVKPL AAKAKPGFYL PPPTTLIGAL SYGKFRGVDN INLGNVYGSP
     AYNFRNIMAT ARLESEGVYT EDIIRNVISY FQRKERRENP RYIYGVIPTG KVYIPNGRLV
     VVYVTDSISK EELEKLCWSI TRIGCKECLA SVENVEVGEA KKVSGRVKTR YYFRDTVKVV
     GRKEFLEYVT FWEENGYIWG KEGSPVRYIL PITTYPLASK EVEVEAKEAY EVGGEYVVFS