ID   CAS5D_THET2             Reviewed;         229 AA.
AC   Q746C2;
DT   04-MAR-2015, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 79.
DE   RecName: Full=CRISPR pre-crRNA endoribonuclease Cas5d {ECO:0000303|PubMed:23006625};
DE            EC=3.1.-.-;
DE   AltName: Full=Pre-crRNA processing endonuclease Cas5d;
GN   Name=cas5d {ECO:0000303|PubMed:23006625}; OrderedLocusNames=TT_P0133;
OS   Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27).
OG   Plasmid pTT27.
OC   Bacteria; Deinococcus-Thermus; Deinococci; Thermales; Thermaceae; Thermus.
OX   NCBI_TaxID=262724;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-163 / DSM 7039 / HB27;
RX   PubMed=15064768; DOI=10.1038/nbt956;
RA   Henne A., Brueggemann H., Raasch C., Wiezer A., Hartsch T., Liesegang H.,
RA   Johann A., Lienard T., Gohl O., Martinez-Arias R., Jacobi C.,
RA   Starkuviene V., Schlenczeck S., Dencker S., Huber R., Klenk H.-P.,
RA   Kramer W., Merkl R., Gottschalk G., Fritz H.-J.;
RT   "The genome sequence of the extreme thermophile Thermus thermophilus.";
RL   Nat. Biotechnol. 22:547-553(2004).
RN   [2]
RP   FUNCTION, PROBABLE REACTION MECHANISM, COFACTOR, RNA-BINDING, AND
RP   MUTAGENESIS OF GLU-23 AND TYR-27.
RC   STRAIN=ATCC BAA-163 / DSM 7039 / HB27;
RX   PubMed=23006625; DOI=10.1261/rna.033100.112;
RA   Garside E.L., Schellenberg M.J., Gesner E.M., Bonanno J.B., Sauder J.M.,
RA   Burley S.K., Almo S.C., Mehta G., MacMillan A.M.;
RT   "Cas5d processes pre-crRNA and is a member of a larger family of CRISPR RNA
RT   endonucleases.";
RL   RNA 18:2020-2028(2012).
CC   -!- FUNCTION: CRISPR (clustered regularly interspaced short palindromic
CC       repeat) is an adaptive immune system that provides protection against
CC       mobile genetic elements (viruses, transposable elements and conjugative
CC       plasmids). CRISPR clusters contain spacers, sequences complementary to
CC       antecedent mobile elements, and target invading nucleic acids. CRISPR
CC       clusters are transcribed and processed into CRISPR RNA (crRNA). This
CC       protein is a sequence-specific endonuclease that cleaves pre-crRNA into
CC       mature crRNA, possibly by an intramolecular attack of the 2'-hydroxyl
CC       group of G26 on the scissile phosphodiester, cutting the precursor 3'
CC       to G26 residue yielding 5'-hydroxyl and 2' and/or 3' ends lacking a
CC       hydroxyl group (perhaps a 2'/3' cyclic phosphodiester). Requires
CC       between 4 and 8 nt downstream of the cleavage site for both binding and
CC       cleavage of pre-crRNA. Substitution with dG at this position abolishes
CC       cleavage but not RNA binding. Does not cleave pre-crRNA associated with
CC       the M.succiniciproducens strain MBEL55E Cas5 protein (AC Q65TW5) CRISPR
CC       locus. {ECO:0000269|PubMed:23006625}.
CC   -!- COFACTOR:
CC       Note=Does not require a metal cofactor. {ECO:0000269|PubMed:23006625};
CC   -!- SIMILARITY: Belongs to the CRISPR-associated protein Cas5 family.
CC       Subtype I-C/Dvulg subfamily. {ECO:0000305}.
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DR   EMBL; AE017222; AAS82463.1; -; Genomic_DNA.
DR   RefSeq; WP_011174431.1; NC_005838.1.
DR   AlphaFoldDB; Q746C2; -.
DR   SMR; Q746C2; -.
DR   STRING; 262724.TT_P0133; -.
DR   EnsemblBacteria; AAS82463; AAS82463; TT_P0133.
DR   KEGG; tth:TT_P0133; -.
DR   eggNOG; ENOG502Z82V; Bacteria.
DR   HOGENOM; CLU_086014_0_0_0; -.
DR   OMA; YLGCREF; -.
DR   OrthoDB; 1619114at2; -.
DR   Proteomes; UP000000592; Plasmid pTT27.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR   GO; GO:0043571; P:maintenance of CRISPR repeat elements; IEA:InterPro.
DR   InterPro; IPR021124; CRISPR-assoc_prot_Cas5.
DR   InterPro; IPR013422; CRISPR-assoc_prot_Cas5_N.
DR   InterPro; IPR010155; CRISPR-assoc_prot_Cas5d.
DR   Pfam; PF09704; Cas_Cas5d; 1.
DR   PIRSF; PIRSF029950; Cas_CT1134; 1.
DR   TIGRFAMs; TIGR01876; cas_Cas5d; 1.
DR   TIGRFAMs; TIGR02593; CRISPR_cas5; 1.
PE   1: Evidence at protein level;
KW   Antiviral defense; Endonuclease; Hydrolase; Nuclease; Plasmid; RNA-binding.
FT   CHAIN           1..229
FT                   /note="CRISPR pre-crRNA endoribonuclease Cas5d"
FT                   /id="PRO_0000432147"
FT   MUTAGEN         23
FT                   /note="E->Q: No longer binds pre-crRNA."
FT                   /evidence="ECO:0000269|PubMed:23006625"
FT   MUTAGEN         27
FT                   /note="Y->F: No change in binding or cleavage of pre-
FT                   crRNA."
FT                   /evidence="ECO:0000269|PubMed:23006625"
SQ   SEQUENCE   229 AA;  26367 MW;  F9538B0DC675D206 CRC64;
     MARLKVKVWG EYACFSRPEF KVERVSYPVP TPSAARGLLE AIFWKPEFRY EVRRIGVLRL
     GTPFALLRNE VGNRMGAKPF FVEDARQQRT SLVLKDVAYL VEADMVLRPH ATDPLPKYLE
     QFERRLKKGQ YHHTPYLGTR EFPAYFSPPD GEVPDGGLNL DLGPMLFDLA FVEDPGRPEL
     TFKRPGRGEV QGYALPLFFH ARIREGWLEV PAEKYQELYR LEEGHAKGA