ID   CAS5_HALVD              Reviewed;         265 AA.
AC   D4GQN7;
DT   04-MAR-2015, integrated into UniProtKB/Swiss-Prot.
DT   18-MAY-2010, sequence version 1.
DT   25-MAY-2022, entry version 41.
DE   RecName: Full=CRISPR-associated protein Cas5 {ECO:0000303|PubMed:24459147};
GN   Name=cas5 {ECO:0000303|PubMed:24459147}; OrderedLocusNames=HVO_A0208;
OS   Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 /
OS   NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii).
OG   Plasmid pHV4.
OC   Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Haloferacales;
OC   Haloferacaceae; Haloferax.
OX   NCBI_TaxID=309800;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM
RC   B-1768 / DS2;
RX   PubMed=20333302; DOI=10.1371/journal.pone.0009605;
RA   Hartman A.L., Norais C., Badger J.H., Delmas S., Haldenby S., Madupu R.,
RA   Robinson J., Khouri H., Ren Q., Lowe T.M., Maupin-Furlow J.,
RA   Pohlschroder M., Daniels C., Pfeiffer F., Allers T., Eisen J.A.;
RT   "The complete genome sequence of Haloferax volcanii DS2, a model
RT   archaeon.";
RL   PLoS ONE 5:E9605-E9605(2010).
RN   [2]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=DS2 / DS70 / H26;
RX   PubMed=22767603; DOI=10.1074/jbc.m112.377002;
RA   Fischer S., Maier L.K., Stoll B., Brendel J., Fischer E., Pfeiffer F.,
RA   Dyall-Smith M., Marchfelder A.;
RT   "An archaeal immune system can detect multiple protospacer adjacent motifs
RT   (PAMs) to target invader DNA.";
RL   J. Biol. Chem. 287:33351-33363(2012).
RN   [3]
RP   FUNCTION, SUBUNIT, AND SUBCELLULAR LOCATION.
RC   STRAIN=DS2 / DS70 / H119;
RX   PubMed=24459147; DOI=10.1074/jbc.m113.508184;
RA   Brendel J., Stoll B., Lange S.J., Sharma K., Lenz C., Stachler A.E.,
RA   Maier L.K., Richter H., Nickel L., Schmitz R.A., Randau L., Allers T.,
RA   Urlaub H., Backofen R., Marchfelder A.;
RT   "A complex of Cas proteins 5, 6, and 7 is required for the biogenesis and
RT   stability of clustered regularly interspaced short palindromic repeats
RT   (CRISPR)-derived RNAs (crRNAs) in Haloferax volcanii.";
RL   J. Biol. Chem. 289:7164-7177(2014).
CC   -!- FUNCTION: CRISPR (clustered regularly interspaced short palindromic
CC       repeat) is an adaptive immune system that provides protection against
CC       mobile genetic elements (viruses, transposable elements and conjugative
CC       plasmids). CRISPR clusters contain sequences complementary to
CC       antecedent mobile elements and target invading nucleic acids. CRISPR
CC       clusters are transcribed and processed into CRISPR RNA (crRNA). Plasmid
CC       targeted by CRISPR locus P1 transform wild-type cells very poorly
CC       (PubMed:22767603). This protein helps process or stabilize pre-crRNA
CC       into individual crRNA units, in vivo Cas5 and Cas6 are also required
CC       for optimal crRNA processing and/or stability (PubMed:24459147).
CC       {ECO:0000269|PubMed:22767603, ECO:0000269|PubMed:24459147}.
CC   -!- SUBUNIT: Component of the Cascade-like complex (Cascade I-B), composed
CC       of Cas5, Cas6, Cas7 and crRNA. {ECO:0000269|PubMed:24459147}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- DISRUPTION PHENOTYPE: Loss of the 8 Cas genes in this locus (cas1,
CC       cas2, cas3, cas4, cas5, cas6, cas7 and cas8b) leads to loss of CRISPR
CC       interference against plasmid targeted by this CRISPR locus, i.e.
CC       plasmid is not destroyed by CRISPR. {ECO:0000269|PubMed:22767603}.
CC   -!- MISCELLANEOUS: There are 3 CRISPR RNA loci in this organism and a
CC       single cas gene locus. A CRISPR-Cas type I-B system.
CC       {ECO:0000303|PubMed:20333302, ECO:0000303|PubMed:24459147}.
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DR   EMBL; CP001955; ADE01943.1; -; Genomic_DNA.
DR   RefSeq; WP_013035118.1; NZ_AOHU01000052.1.
DR   AlphaFoldDB; D4GQN7; -.
DR   SMR; D4GQN7; -.
DR   STRING; 309800.C498_09716; -.
DR   EnsemblBacteria; ADE01943; ADE01943; HVO_A0208.
DR   GeneID; 8923454; -.
DR   KEGG; hvo:HVO_A0208; -.
DR   eggNOG; arCOG02758; Archaea.
DR   HOGENOM; CLU_090888_0_0_2; -.
DR   OMA; PWGHFRR; -.
DR   OrthoDB; 82328at2157; -.
DR   Proteomes; UP000008243; Plasmid pHV4.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR   GO; GO:0043571; P:maintenance of CRISPR repeat elements; IMP:CACAO.
DR   InterPro; IPR021124; CRISPR-assoc_prot_Cas5.
DR   InterPro; IPR013421; CRISPR-assoc_prot_Cas5_HALMA.
DR   InterPro; IPR013422; CRISPR-assoc_prot_Cas5_N.
DR   Pfam; PF09704; Cas_Cas5d; 1.
DR   TIGRFAMs; TIGR02592; cas_Cas5h; 1.
DR   TIGRFAMs; TIGR02593; CRISPR_cas5; 1.
PE   1: Evidence at protein level;
KW   Antiviral defense; Cytoplasm; Plasmid; Reference proteome.
FT   CHAIN           1..265
FT                   /note="CRISPR-associated protein Cas5"
FT                   /id="PRO_0000432154"
SQ   SEQUENCE   265 AA;  29323 MW;  91A874021898A8F9 CRC64;
     MSPHIDANGV PIDCLSFTVR STWGHFKRVG RSVTKQTYRI PPRTTVAGML AAIVGADRDS
     YYDVFGADNS AIAITPLSDL RTVNIPTTGL GTDPKQDVTE TAGSWRTYKL TYQNTTGDRQ
     LHSYEVLADP AYRIDVALEN EEFYDSLREH LVNGTSIYPP SLGKSEYLAV IEDVEVDREP
     EVQDIDETLD IDSVVPISLS EAVPQGKVTY GVERSPAVMV RESGGRRTTR FDDYVYAQQA
     SNPVRIGEQT DVAPVQVGDR TVVFR