CAS6A_METJA
ID CAS6A_METJA Reviewed; 254 AA.
AC Q57820;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 25-MAY-2022, entry version 101.
DE RecName: Full=CRISPR-associated endoribonuclease Cas6 1;
DE EC=3.1.-.-;
GN Name=cas6a; OrderedLocusNames=MJ0375;
OS Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM
OS 10045 / NBRC 100440) (Methanococcus jannaschii).
OC Archaea; Euryarchaeota; Methanomada group; Methanococci; Methanococcales;
OC Methanocaldococcaceae; Methanocaldococcus.
OX NCBI_TaxID=243232;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440;
RX PubMed=8688087; DOI=10.1126/science.273.5278.1058;
RA Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G.,
RA Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R.,
RA Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R.,
RA Kirkness E.F., Weinstock K.G., Merrick J.M., Glodek A., Scott J.L.,
RA Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R.,
RA Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D.,
RA Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P.,
RA Fraser C.M., Smith H.O., Woese C.R., Venter J.C.;
RT "Complete genome sequence of the methanogenic archaeon, Methanococcus
RT jannaschii.";
RL Science 273:1058-1073(1996).
CC -!- FUNCTION: CRISPR (clustered regularly interspaced short palindromic
CC repeat) is an adaptive immune system that provides protection against
CC mobile genetic elements (viruses, transposable elements and conjugative
CC plasmids). CRISPR clusters contain sequences complementary to
CC antecedent mobile elements and target invading nucleic acids. CRISPR
CC clusters are transcribed and processed into CRISPR RNA (crRNA). This
CC protein processes pre-crRNA into individual crRNA units.
CC {ECO:0000250|UniProtKB:Q8U1S4}.
CC -!- SIMILARITY: Belongs to the CRISPR-associated protein Cas6/Cse3/CasE
CC family. {ECO:0000305}.
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DR EMBL; L77117; AAB98364.1; -; Genomic_DNA.
DR PIR; G64346; G64346.
DR PDB; 5YI6; X-ray; 1.85 A; A/B/C/D=1-254.
DR PDBsum; 5YI6; -.
DR AlphaFoldDB; Q57820; -.
DR SMR; Q57820; -.
DR STRING; 243232.MJ_0375; -.
DR EnsemblBacteria; AAB98364; AAB98364; MJ_0375.
DR KEGG; mja:MJ_0375; -.
DR eggNOG; arCOG04342; Archaea.
DR HOGENOM; CLU_089858_1_1_2; -.
DR InParanoid; Q57820; -.
DR OMA; FGMVKVD; -.
DR PhylomeDB; Q57820; -.
DR Proteomes; UP000000805; Chromosome.
DR GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR Gene3D; 3.30.70.1890; -; 1.
DR InterPro; IPR010156; CRISPR-assoc_prot_Cas6.
DR InterPro; IPR045747; CRISPR-assoc_prot_Cas6_N_sf.
DR PANTHER; PTHR36984; PTHR36984; 1.
DR Pfam; PF01881; Cas_Cas6; 1.
DR PIRSF; PIRSF005054; PF1131; 1.
DR TIGRFAMs; TIGR01877; cas_cas6; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Antiviral defense; Endonuclease; Hydrolase; Nuclease;
KW Reference proteome; RNA-binding.
FT CHAIN 1..254
FT /note="CRISPR-associated endoribonuclease Cas6 1"
FT /id="PRO_0000106838"
FT ACT_SITE 32
FT /note="Proton acceptor"
FT /evidence="ECO:0000250|UniProtKB:Q8U1S4"
FT ACT_SITE 47
FT /note="Proton donor"
FT /evidence="ECO:0000250|UniProtKB:Q8U1S4"
FT SITE 53
FT /note="Transition state stabilizer"
FT /evidence="ECO:0000250|UniProtKB:Q8U1S4"
FT STRAND 5..12
FT /evidence="ECO:0007829|PDB:5YI6"
FT STRAND 17..20
FT /evidence="ECO:0007829|PDB:5YI6"
FT TURN 21..23
FT /evidence="ECO:0007829|PDB:5YI6"
FT HELIX 24..35
FT /evidence="ECO:0007829|PDB:5YI6"
FT HELIX 40..43
FT /evidence="ECO:0007829|PDB:5YI6"
FT TURN 44..46
FT /evidence="ECO:0007829|PDB:5YI6"
FT HELIX 47..49
FT /evidence="ECO:0007829|PDB:5YI6"
FT STRAND 63..67
FT /evidence="ECO:0007829|PDB:5YI6"
FT STRAND 69..86
FT /evidence="ECO:0007829|PDB:5YI6"
FT HELIX 88..101
FT /evidence="ECO:0007829|PDB:5YI6"
FT STRAND 103..106
FT /evidence="ECO:0007829|PDB:5YI6"
FT STRAND 109..118
FT /evidence="ECO:0007829|PDB:5YI6"
FT STRAND 126..132
FT /evidence="ECO:0007829|PDB:5YI6"
FT STRAND 137..142
FT /evidence="ECO:0007829|PDB:5YI6"
FT STRAND 145..150
FT /evidence="ECO:0007829|PDB:5YI6"
FT HELIX 158..174
FT /evidence="ECO:0007829|PDB:5YI6"
FT STRAND 182..195
FT /evidence="ECO:0007829|PDB:5YI6"
FT STRAND 197..199
FT /evidence="ECO:0007829|PDB:5YI6"
FT STRAND 201..213
FT /evidence="ECO:0007829|PDB:5YI6"
FT HELIX 215..224
FT /evidence="ECO:0007829|PDB:5YI6"
FT STRAND 226..228
FT /evidence="ECO:0007829|PDB:5YI6"
FT HELIX 230..232
FT /evidence="ECO:0007829|PDB:5YI6"
FT STRAND 237..240
FT /evidence="ECO:0007829|PDB:5YI6"
SQ SEQUENCE 254 AA; 30184 MW; D9BCD0EB681148E5 CRC64;
MRESMRIELE LQTDNFTVIP YNHQYYLASA IYNKIHSANP AYAKRLHNYQ KFKFFTFSLL
QIRKRVIRKE GIETIDGKAY LYISSPNNEF IENFVAGLLE DGKLRVGNVE FFVRKAKILP
IPKKFNILKT ISPIYLKTMI ETEDGLKTYD LLPNNSKFYE NLKNNLKKKY EAFYNEKCDM
NFEFEVLKFR PKRMRIKNDI YCRCSEMVFK VWGDYDLIKF GYECGFGEKN SMGFGMVVNV
EDKNQKNKKL KTKI
