ID   CAS6B_METJA             Reviewed;         241 AA.
AC   Q58631;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 93.
DE   RecName: Full=CRISPR-associated endoribonuclease Cas6 2;
DE            EC=3.1.-.-;
GN   Name=cas6b; OrderedLocusNames=MJ1234;
OS   Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM
OS   10045 / NBRC 100440) (Methanococcus jannaschii).
OC   Archaea; Euryarchaeota; Methanomada group; Methanococci; Methanococcales;
OC   Methanocaldococcaceae; Methanocaldococcus.
OX   NCBI_TaxID=243232;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440;
RX   PubMed=8688087; DOI=10.1126/science.273.5278.1058;
RA   Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G.,
RA   Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R.,
RA   Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R.,
RA   Kirkness E.F., Weinstock K.G., Merrick J.M., Glodek A., Scott J.L.,
RA   Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R.,
RA   Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D.,
RA   Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P.,
RA   Fraser C.M., Smith H.O., Woese C.R., Venter J.C.;
RT   "Complete genome sequence of the methanogenic archaeon, Methanococcus
RT   jannaschii.";
RL   Science 273:1058-1073(1996).
CC   -!- FUNCTION: CRISPR (clustered regularly interspaced short palindromic
CC       repeat) is an adaptive immune system that provides protection against
CC       mobile genetic elements (viruses, transposable elements and conjugative
CC       plasmids). CRISPR clusters contain sequences complementary to
CC       antecedent mobile elements and target invading nucleic acids. CRISPR
CC       clusters are transcribed and processed into CRISPR RNA (crRNA). This
CC       protein processes pre-crRNA into individual crRNA units.
CC       {ECO:0000250|UniProtKB:Q8U1S4}.
CC   -!- SIMILARITY: Belongs to the CRISPR-associated protein Cas6/Cse3/CasE
CC       family. {ECO:0000305}.
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DR   EMBL; L77117; AAB99238.1; -; Genomic_DNA.
DR   PIR; A64454; A64454.
DR   RefSeq; WP_010870746.1; NC_000909.1.
DR   AlphaFoldDB; Q58631; -.
DR   SMR; Q58631; -.
DR   STRING; 243232.MJ_1234; -.
DR   DNASU; 1452130; -.
DR   EnsemblBacteria; AAB99238; AAB99238; MJ_1234.
DR   GeneID; 1452130; -.
DR   KEGG; mja:MJ_1234; -.
DR   eggNOG; arCOG04342; Archaea.
DR   HOGENOM; CLU_089858_1_0_2; -.
DR   InParanoid; Q58631; -.
DR   OMA; NIGEMPF; -.
DR   OrthoDB; 59332at2157; -.
DR   PhylomeDB; Q58631; -.
DR   Proteomes; UP000000805; Chromosome.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.70.1890; -; 1.
DR   InterPro; IPR010156; CRISPR-assoc_prot_Cas6.
DR   InterPro; IPR045747; CRISPR-assoc_prot_Cas6_N_sf.
DR   PANTHER; PTHR36984; PTHR36984; 1.
DR   Pfam; PF01881; Cas_Cas6; 1.
DR   PIRSF; PIRSF005054; PF1131; 1.
DR   TIGRFAMs; TIGR01877; cas_cas6; 1.
PE   3: Inferred from homology;
KW   Antiviral defense; Endonuclease; Hydrolase; Nuclease; Reference proteome;
KW   RNA-binding.
FT   CHAIN           1..241
FT                   /note="CRISPR-associated endoribonuclease Cas6 2"
FT                   /id="PRO_0000107230"
FT   ACT_SITE        28
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250|UniProtKB:Q8U1S4"
FT   ACT_SITE        40
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250|UniProtKB:Q8U1S4"
FT   SITE            46
FT                   /note="Transition state stabilizer"
FT                   /evidence="ECO:0000250|UniProtKB:Q8U1S4"
SQ   SEQUENCE   241 AA;  28840 MW;  23AD8F19448CBFC6 CRC64;
     MRLKLSLTPK QDFSFDKINK HTIQGFIYSL LKDTEFGEMH NQPRFKFWCF SDIFPPNDFV
     KGEDKYLLIS SPREEFINVL YERLDNLEEV NLNNFKFEVS ELKKFDLKVK NKFITGSPIV
     LYKDKDRGEY IKFYDDDFDL MFFVQRLQDN AVKKYKAFYN EEPVLNGFIF DRISPRVRNG
     RVDVYVRIAK KGREFLVVGT TWKLLEKIKI RKEERKFYKF IMDCGLGEKN SLGFGFINPI
     K