ID   CAS6_ECOUT              Reviewed;         184 AA.
AC   Q1RE32;
DT   13-JUN-2012, integrated into UniProtKB/Swiss-Prot.
DT   16-MAY-2006, sequence version 1.
DT   25-MAY-2022, entry version 65.
DE   RecName: Full=CRISPR-associated endonuclease Cas6/Csy4;
DE            EC=3.1.-.-;
DE   AltName: Full=crRNA endonuclease;
GN   Name=cas6f; Synonyms=csy4; OrderedLocusNames=UTI89_C0896;
OS   Escherichia coli (strain UTI89 / UPEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=364106;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=UTI89 / UPEC;
RX   PubMed=16585510; DOI=10.1073/pnas.0600938103;
RA   Chen S.L., Hung C.-S., Xu J., Reigstad C.S., Magrini V., Sabo A.,
RA   Blasiar D., Bieri T., Meyer R.R., Ozersky P., Armstrong J.R., Fulton R.S.,
RA   Latreille J.P., Spieth J., Hooton T.M., Mardis E.R., Hultgren S.J.,
RA   Gordon J.I.;
RT   "Identification of genes subject to positive selection in uropathogenic
RT   strains of Escherichia coli: a comparative genomics approach.";
RL   Proc. Natl. Acad. Sci. U.S.A. 103:5977-5982(2006).
RN   [2]
RP   FUNCTION IN CRRNA FORMATION, FUNCTION IN INHIBITION OF BIOFILM FORMATION,
RP   AND MUTAGENESIS OF HIS-29.
RC   STRAIN=UTI89 / UPEC;
RX   PubMed=21398535; DOI=10.1128/jb.01411-10;
RA   Cady K.C., O'Toole G.A.;
RT   "Non-identity-mediated CRISPR-bacteriophage interaction mediated via the
RT   Csy and Cas3 proteins.";
RL   J. Bacteriol. 193:3433-3445(2011).
CC   -!- FUNCTION: CRISPR (clustered regularly interspaced short palindromic
CC       repeat) is an adaptive immune system that provides protection against
CC       mobile genetic elements (viruses, transposable elements and conjugative
CC       plasmids). CRISPR clusters contain sequences complementary to
CC       antecedent mobile elements and target invading nucleic acids. CRISPR
CC       clusters are transcribed and processed into CRISPR RNA (crRNA).
CC       Processes pre-crRNA into individual crRNA units (By similarity).
CC       {ECO:0000250}.
CC   -!- FUNCTION: Complements a csy4 disruption in Pseudomonas aeruginosa PA14,
CC       restoring inhibition of biofilm formation and generation of crRNA.
CC       {ECO:0000269|PubMed:21398535}.
CC   -!- SIMILARITY: Belongs to the CRISPR-associated endoribonuclease Cas6
CC       family. Cas6f/Csy4, subtype I-F/Ypest subfamily. {ECO:0000305}.
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DR   EMBL; CP000243; ABE06382.1; -; Genomic_DNA.
DR   RefSeq; WP_000350182.1; NC_007946.1.
DR   AlphaFoldDB; Q1RE32; -.
DR   SMR; Q1RE32; -.
DR   EnsemblBacteria; ABE06382; ABE06382; UTI89_C0896.
DR   KEGG; eci:UTI89_C0896; -.
DR   HOGENOM; CLU_108958_0_0_6; -.
DR   OMA; HYIEIRV; -.
DR   Proteomes; UP000001952; Chromosome.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR   GO; GO:0043571; P:maintenance of CRISPR repeat elements; IEA:InterPro.
DR   CDD; cd09739; Cas6_I-F; 1.
DR   Gene3D; 3.30.70.2540; -; 1.
DR   InterPro; IPR013396; CRISPR-assoc_prot_Csy4.
DR   InterPro; IPR042564; CRISPR-Cas6/Csy4_sf.
DR   Pfam; PF09618; Cas_Csy4; 1.
DR   TIGRFAMs; TIGR02563; cas_Csy4; 1.
PE   1: Evidence at protein level;
KW   Antiviral defense; Endonuclease; Hydrolase; Nuclease; RNA-binding.
FT   CHAIN           1..184
FT                   /note="CRISPR-associated endonuclease Cas6/Csy4"
FT                   /id="PRO_0000417881"
FT   MUTAGEN         29
FT                   /note="H->A: Does not complement Pseudomonas aeruginosa
FT                   csy4 deletion, no production of crRNA."
FT                   /evidence="ECO:0000269|PubMed:21398535"
SQ   SEQUENCE   184 AA;  20720 MW;  5F4740A52C4C85E6 CRC64;
     MDHYLEIRVL PDPEFSSEML MAALFAKLHR VLGARGQGDI GVSFPDVNVM PGARLRLHGS
     AQALQALEAS TWRKGLTDYC QCSPVTPVPE IKGWRVVSRV QVKSNPQRLL RRSVKKGWLT
     EEQAIERLAT QAEQRTDLPF LNMKSLSSQQ LFKLFIRHGD LLKEPVKGEF SSYGLSATAT
     IPWF