ID   Y520_METJA              Reviewed;         295 AA.
AC   Q57940;
DT   16-JAN-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 106.
DE   RecName: Full=Putative NADH-ubiquinone oxidoreductase MJ0520;
DE            EC=7.1.1.2;
GN   OrderedLocusNames=MJ0520;
OS   Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM
OS   10045 / NBRC 100440) (Methanococcus jannaschii).
OC   Archaea; Euryarchaeota; Methanomada group; Methanococci; Methanococcales;
OC   Methanocaldococcaceae; Methanocaldococcus.
OX   NCBI_TaxID=243232;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440;
RX   PubMed=8688087; DOI=10.1126/science.273.5278.1058;
RA   Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G.,
RA   Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R.,
RA   Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R.,
RA   Kirkness E.F., Weinstock K.G., Merrick J.M., Glodek A., Scott J.L.,
RA   Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R.,
RA   Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D.,
RA   Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P.,
RA   Fraser C.M., Smith H.O., Woese C.R., Venter J.C.;
RT   "Complete genome sequence of the methanogenic archaeon, Methanococcus
RT   jannaschii.";
RL   Science 273:1058-1073(1996).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ubiquinone + 5 H(+)(in) + NADH = a ubiquinol + 4 H(+)(out) +
CC         NAD(+); Xref=Rhea:RHEA:29091, Rhea:RHEA-COMP:9565, Rhea:RHEA-
CC         COMP:9566, ChEBI:CHEBI:15378, ChEBI:CHEBI:16389, ChEBI:CHEBI:17976,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=7.1.1.2;
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the complex I subunit 1 family. {ECO:0000305}.
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DR   EMBL; L77117; AAB98508.1; -; Genomic_DNA.
DR   PIR; H64364; H64364.
DR   AlphaFoldDB; Q57940; -.
DR   SMR; Q57940; -.
DR   STRING; 243232.MJ_0520; -.
DR   EnsemblBacteria; AAB98508; AAB98508; MJ_0520.
DR   KEGG; mja:MJ_0520; -.
DR   eggNOG; arCOG01545; Archaea.
DR   HOGENOM; CLU_015134_0_2_2; -.
DR   InParanoid; Q57940; -.
DR   OMA; KIMARIQ; -.
DR   PhylomeDB; Q57940; -.
DR   Proteomes; UP000000805; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR   GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:UniProtKB-EC.
DR   InterPro; IPR001694; NADH_UbQ_OxRdtase_su1/FPO.
DR   Pfam; PF00146; NADHdh; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Membrane; NAD; Reference proteome; Translocase;
KW   Transmembrane; Transmembrane helix; Ubiquinone.
FT   CHAIN           1..295
FT                   /note="Putative NADH-ubiquinone oxidoreductase MJ0520"
FT                   /id="PRO_0000117536"
FT   TRANSMEM        8..28
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        69..89
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        129..149
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        163..183
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        199..219
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        220..240
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        243..263
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        273..293
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   295 AA;  31909 MW;  F9903DBC914DFAD6 CRC64;
     MMLMDTSLIG AINLTIHAFL VGSLLLGLHR KIMARIQGRP GPPIIQYLLH TLKFYVKEIT
     FPITAGNPLY IFVALLDIAI WLAALIIAID FKSSLLIIIG IYVLQKIVEH GCGLSSGSPY
     GKIGGVRSVF SAAAEVPLFA VVAAIYLTTH SVLISDILSY QEIHGSLLFK MPICAFAFFI
     LLVSKAPNSP FGIVKGKDIV SGYMTEHYGL LGAIIYIAEA IAYFVLLWLF IAVFIGPLVI
     NSPVLTLAVM VVMTVILAFV NGLTPLLAPH HSVMLQMTIA GLVLCDVLYR LIVGG