ID   CAS6_HALMT              Reviewed;         270 AA.
AC   I3RB26;
DT   04-MAR-2015, integrated into UniProtKB/Swiss-Prot.
DT   05-SEP-2012, sequence version 1.
DT   25-MAY-2022, entry version 40.
DE   RecName: Full=CRISPR-associated endoribonuclease Cas6;
DE            EC=3.1.-.-;
GN   Name=cas6; OrderedLocusNames=HFX_6314;
OS   Haloferax mediterranei (strain ATCC 33500 / DSM 1411 / JCM 8866 / NBRC
OS   14739 / NCIMB 2177 / R-4) (Halobacterium mediterranei).
OG   Plasmid pHM500.
OC   Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Haloferacales;
OC   Haloferacaceae; Haloferax.
OX   NCBI_TaxID=523841;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 33500 / DSM 1411 / JCM 8866 / NBRC 14739 / NCIMB 2177 / R-4;
RX   PubMed=22843593; DOI=10.1128/jb.00880-12;
RA   Han J., Zhang F., Hou J., Liu X., Li M., Liu H., Cai L., Zhang B., Chen Y.,
RA   Zhou J., Hu S., Xiang H.;
RT   "Complete genome sequence of the metabolically versatile halophilic
RT   archaeon Haloferax mediterranei, a poly(3-hydroxybutyrate-co-3-
RT   hydroxyvalerate) producer.";
RL   J. Bacteriol. 194:4463-4464(2012).
RN   [2]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 33500 / DSM 1411 / JCM 8866 / NBRC 14739 / NCIMB 2177 / R-4;
RX   PubMed=23243301; DOI=10.1128/jb.01688-12;
RA   Li M., Liu H., Han J., Liu J., Wang R., Zhao D., Zhou J., Xiang H.;
RT   "Characterization of CRISPR RNA biogenesis and Cas6 cleavage-mediated
RT   inhibition of a provirus in the haloarchaeon Haloferax mediterranei.";
RL   J. Bacteriol. 195:867-875(2013).
CC   -!- FUNCTION: CRISPR (clustered regularly interspaced short palindromic
CC       repeat) is an adaptive immune system that provides protection against
CC       mobile genetic elements (viruses, transposable elements and conjugative
CC       plasmids). CRISPR clusters contain sequences complementary to
CC       antecedent mobile elements and target invading nucleic acids. CRISPR
CC       clusters are transcribed and processed into CRISPR RNA (crRNA). This
CC       protein probably processes pre-crRNA into individual crRNA units, and
CC       also plays a role in generation or stability of the CRISPR leader-
CC       repeat transcript. Cleaves an endogenous (non-invading) RNA; cuts a
CC       specific site within a sequence nearly identical to the cognate CRISPR
CC       repeat sequence in the 5' UTR of the transcript of provirus gene phiH,
CC       found in provirus 1. This digestion might be responsible for inhibiting
CC       (defective) provirus 1 excision. {ECO:0000269|PubMed:23243301}.
CC   -!- DISRUPTION PHENOTYPE: Does not produce mature length crRNA; precursor
CC       is non-specifically degraded. No accumulation of the CRISPR leader-
CC       repeat transcript. Allows excision of defective provirus 1 from its
CC       chromosomal locus (detected as restoration of the attachment site
CC       attA). {ECO:0000269|PubMed:23243301}.
CC   -!- MISCELLANEOUS: There are 6 CRISPR RNA loci in this organism and a
CC       single cas gene locus. A CRISPR-Cas type I-B system.
CC       {ECO:0000303|PubMed:23243301}.
CC   -!- SIMILARITY: Belongs to the CRISPR-associated protein Cas6/Cse3/CasE
CC       family. {ECO:0000305}.
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DR   EMBL; CP001871; AFK21436.1; -; Genomic_DNA.
DR   RefSeq; WP_014732817.1; NZ_CP039140.1.
DR   AlphaFoldDB; I3RB26; -.
DR   SMR; I3RB26; -.
DR   EnsemblBacteria; AFK21436; AFK21436; HFX_6314.
DR   GeneID; 40158021; -.
DR   KEGG; hme:HFX_6314; -.
DR   HOGENOM; CLU_089858_1_0_2; -.
DR   OMA; NIGEMPF; -.
DR   OrthoDB; 59332at2157; -.
DR   Proteomes; UP000006469; Plasmid pHM500.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.70.1890; -; 1.
DR   InterPro; IPR010156; CRISPR-assoc_prot_Cas6.
DR   InterPro; IPR045747; CRISPR-assoc_prot_Cas6_N_sf.
DR   PANTHER; PTHR36984; PTHR36984; 1.
DR   Pfam; PF01881; Cas_Cas6; 1.
DR   TIGRFAMs; TIGR01877; cas_cas6; 1.
PE   3: Inferred from homology;
KW   Antiviral defense; Endonuclease; Hydrolase; Nuclease; Plasmid; RNA-binding.
FT   CHAIN           1..270
FT                   /note="CRISPR-associated endoribonuclease Cas6"
FT                   /id="PRO_0000432152"
FT   ACT_SITE        41
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250|UniProtKB:D4GQN4"
SQ   SEQUENCE   270 AA;  30616 MW;  4DEED8441FF75CE4 CRC64;
     MRLMVHLEAQ RDATYQTAYH HKLRGRVWRA LEGSKFDSEH DNGHPLGLAF SNIFPWGKIQ
     EGDRRSLLFA SPREDLLATI ARGLQRNRDF NVGEMSFEVD DLSQLTVDVG EPGTRGVIET
     ATGVVVRLTP EHREQYGIEA EHDAPTYWRP EHTIKPFKDA IRANLQHKHD RFAHEYQDGP
     TDVDGELFEG YDLLKTYALP VTVTTGTTLD VVLSKWRFDY RVRNDAHRHH LNLALDAGIG
     GRNGLGFGFS NIVEKTKPGE SELERADAFA