Y5224_ARATH
ID Y5224_ARATH Reviewed; 253 AA.
AC Q94EG6; Q9LZK8;
DT 30-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 25-MAY-2022, entry version 125.
DE RecName: Full=Uncharacterized protein At5g02240;
GN OrderedLocusNames=At5g02240; ORFNames=T7H20_290;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, CLEAVAGE OF INITIATOR
RP METHIONINE [LARGE SCALE ANALYSIS], AND IDENTIFICATION BY MASS SPECTROMETRY
RP [LARGE SCALE ANALYSIS].
RX PubMed=22223895; DOI=10.1074/mcp.m111.015131;
RA Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T.,
RA Giglione C.;
RT "Comparative large-scale characterisation of plant vs. mammal proteins
RT reveals similar and idiosyncratic N-alpha acetylation features.";
RL Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012).
RN [6]
RP X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 2-253, AND SUBUNIT.
RG Center for eukaryotic structural genomics (CESG);
RT "X-ray structure of gene product from Arabidopsis thaliana At5g02240.";
RL Submitted (FEB-2005) to the PDB data bank.
CC -!- SUBUNIT: Homodimer. {ECO:0000305|Ref.6}.
CC -!- SIMILARITY: Belongs to the NAD(P)-dependent epimerase/dehydratase
CC family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB82997.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AL162508; CAB82997.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED90449.1; -; Genomic_DNA.
DR EMBL; AY142045; AAM98309.1; -; mRNA.
DR EMBL; BT000751; AAN31891.1; -; mRNA.
DR EMBL; AK221714; BAD95439.1; -; mRNA.
DR EMBL; AF410336; AAK95322.1; -; mRNA.
DR PIR; T48245; T48245.
DR RefSeq; NP_568098.1; NM_120302.3.
DR PDB; 1XQ6; X-ray; 1.80 A; A/B=2-253.
DR PDB; 1YBM; X-ray; 2.10 A; A/B=2-253.
DR PDB; 2Q46; X-ray; 1.80 A; A/B=2-253.
DR PDB; 2Q4B; X-ray; 2.10 A; A/B=2-253.
DR PDBsum; 1XQ6; -.
DR PDBsum; 1YBM; -.
DR PDBsum; 2Q46; -.
DR PDBsum; 2Q4B; -.
DR AlphaFoldDB; Q94EG6; -.
DR SMR; Q94EG6; -.
DR STRING; 3702.AT5G02240.1; -.
DR iPTMnet; Q94EG6; -.
DR MetOSite; Q94EG6; -.
DR PaxDb; Q94EG6; -.
DR PRIDE; Q94EG6; -.
DR ProteomicsDB; 243013; -.
DR DNASU; 830851; -.
DR EnsemblPlants; AT5G02240.1; AT5G02240.1; AT5G02240.
DR GeneID; 830851; -.
DR Gramene; AT5G02240.1; AT5G02240.1; AT5G02240.
DR KEGG; ath:AT5G02240; -.
DR Araport; AT5G02240; -.
DR TAIR; locus:2185228; AT5G02240.
DR eggNOG; KOG1203; Eukaryota.
DR HOGENOM; CLU_025711_0_0_1; -.
DR InParanoid; Q94EG6; -.
DR OMA; FIFEDGQ; -.
DR OrthoDB; 1166292at2759; -.
DR PhylomeDB; Q94EG6; -.
DR EvolutionaryTrace; Q94EG6; -.
DR PRO; PR:Q94EG6; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q94EG6; baseline and differential.
DR Genevisible; Q94EG6; AT.
DR GO; GO:0048046; C:apoplast; HDA:TAIR.
DR GO; GO:0009570; C:chloroplast stroma; HDA:TAIR.
DR GO; GO:0005829; C:cytosol; HDA:TAIR.
DR GO; GO:0000325; C:plant-type vacuole; HDA:TAIR.
DR GO; GO:0005886; C:plasma membrane; HDA:TAIR.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:InterPro.
DR GO; GO:0009737; P:response to abscisic acid; IEP:TAIR.
DR InterPro; IPR016040; NAD(P)-bd_dom.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR044163; SARED1-like.
DR PANTHER; PTHR14194; PTHR14194; 1.
DR Pfam; PF13460; NAD_binding_10; 1.
DR SUPFAM; SSF51735; SSF51735; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Acetylation; Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0007744|PubMed:22223895"
FT CHAIN 2..253
FT /note="Uncharacterized protein At5g02240"
FT /id="PRO_0000183268"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0007744|PubMed:22223895"
FT STRAND 6..11
FT /evidence="ECO:0007829|PDB:1XQ6"
FT HELIX 15..26
FT /evidence="ECO:0007829|PDB:1XQ6"
FT TURN 27..30
FT /evidence="ECO:0007829|PDB:1XQ6"
FT STRAND 32..38
FT /evidence="ECO:0007829|PDB:1XQ6"
FT HELIX 40..45
FT /evidence="ECO:0007829|PDB:1XQ6"
FT STRAND 52..54
FT /evidence="ECO:0007829|PDB:1XQ6"
FT HELIX 60..67
FT /evidence="ECO:0007829|PDB:1XQ6"
FT STRAND 71..75
FT /evidence="ECO:0007829|PDB:1XQ6"
FT HELIX 105..108
FT /evidence="ECO:0007829|PDB:1XQ6"
FT HELIX 111..123
FT /evidence="ECO:0007829|PDB:1XQ6"
FT STRAND 126..133
FT /evidence="ECO:0007829|PDB:1XQ6"
FT TURN 134..137
FT /evidence="ECO:0007829|PDB:1XQ6"
FT HELIX 142..148
FT /evidence="ECO:0007829|PDB:1XQ6"
FT HELIX 151..163
FT /evidence="ECO:0007829|PDB:1XQ6"
FT STRAND 165..167
FT /evidence="ECO:0007829|PDB:1XQ6"
FT STRAND 169..174
FT /evidence="ECO:0007829|PDB:1XQ6"
FT STRAND 176..178
FT /evidence="ECO:0007829|PDB:1XQ6"
FT STRAND 182..185
FT /evidence="ECO:0007829|PDB:1XQ6"
FT STRAND 187..191
FT /evidence="ECO:0007829|PDB:1XQ6"
FT HELIX 194..197
FT /evidence="ECO:0007829|PDB:1XQ6"
FT STRAND 202..204
FT /evidence="ECO:0007829|PDB:1XQ6"
FT HELIX 205..215
FT /evidence="ECO:0007829|PDB:1XQ6"
FT HELIX 219..221
FT /evidence="ECO:0007829|PDB:1XQ6"
FT STRAND 224..230
FT /evidence="ECO:0007829|PDB:1XQ6"
FT TURN 233..235
FT /evidence="ECO:0007829|PDB:1XQ6"
FT HELIX 242..247
FT /evidence="ECO:0007829|PDB:1XQ6"
SQ SEQUENCE 253 AA; 27103 MW; F42BDB49982CC75C CRC64;
MANLPTVLVT GASGRTGQIV YKKLKEGSDK FVAKGLVRSA QGKEKIGGEA DVFIGDITDA
DSINPAFQGI DALVILTSAV PKMKPGFDPT KGGRPEFIFE DGQYPEQVDW IGQKNQIDAA
KVAGVKHIVV VGSMGGTNPD HPLNKLGNGN ILVWKRKAEQ YLADSGTPYT IIRAGGLLDK
EGGVRELLVG KDDELLQTDT KTVPRADVAE VCIQALLFEE AKNKAFDLGS KPEGTSTPTK
DFKALFSQVT SRF
