CAS6_HALVD
ID CAS6_HALVD Reviewed; 272 AA.
AC D4GQN4;
DT 04-MAR-2015, integrated into UniProtKB/Swiss-Prot.
DT 18-MAY-2010, sequence version 1.
DT 25-MAY-2022, entry version 42.
DE RecName: Full=CRISPR-associated endoribonuclease Cas6 {ECO:0000303|PubMed:24459147};
DE EC=3.1.-.-;
GN Name=cas6 {ECO:0000303|PubMed:24459147}; OrderedLocusNames=HVO_A0205;
OS Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 /
OS NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii).
OG Plasmid pHV4.
OC Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Haloferacales;
OC Haloferacaceae; Haloferax.
OX NCBI_TaxID=309800;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM
RC B-1768 / DS2;
RX PubMed=20333302; DOI=10.1371/journal.pone.0009605;
RA Hartman A.L., Norais C., Badger J.H., Delmas S., Haldenby S., Madupu R.,
RA Robinson J., Khouri H., Ren Q., Lowe T.M., Maupin-Furlow J.,
RA Pohlschroder M., Daniels C., Pfeiffer F., Allers T., Eisen J.A.;
RT "The complete genome sequence of Haloferax volcanii DS2, a model
RT archaeon.";
RL PLoS ONE 5:E9605-E9605(2010).
RN [2]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=DS2 / DS70 / H26;
RX PubMed=22767603; DOI=10.1074/jbc.m112.377002;
RA Fischer S., Maier L.K., Stoll B., Brendel J., Fischer E., Pfeiffer F.,
RA Dyall-Smith M., Marchfelder A.;
RT "An archaeal immune system can detect multiple protospacer adjacent motifs
RT (PAMs) to target invader DNA.";
RL J. Biol. Chem. 287:33351-33363(2012).
RN [3]
RP FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, INDUCTION, DISRUPTION PHENOTYPE,
RP ACTIVE SITE, AND MUTAGENESIS OF HIS-41; SER-115; SER-224; GLY-256 AND
RP GLY-258.
RC STRAIN=DS2 / DS70 / H119;
RX PubMed=24459147; DOI=10.1074/jbc.m113.508184;
RA Brendel J., Stoll B., Lange S.J., Sharma K., Lenz C., Stachler A.E.,
RA Maier L.K., Richter H., Nickel L., Schmitz R.A., Randau L., Allers T.,
RA Urlaub H., Backofen R., Marchfelder A.;
RT "A complex of Cas proteins 5, 6, and 7 is required for the biogenesis and
RT stability of clustered regularly interspaced short palindromic repeats
RT (CRISPR)-derived RNAs (crRNAs) in Haloferax volcanii.";
RL J. Biol. Chem. 289:7164-7177(2014).
CC -!- FUNCTION: CRISPR (clustered regularly interspaced short palindromic
CC repeat) is an adaptive immune system that provides protection against
CC mobile genetic elements (viruses, transposable elements and conjugative
CC plasmids). CRISPR clusters contain sequences complementary to
CC antecedent mobile elements and target invading nucleic acids. CRISPR
CC clusters are transcribed and processed into CRISPR RNA (crRNA). Plasmid
CC targeted by CRISPR locus P1 transform wild-type cells very poorly
CC (PubMed:22767603). This protein processes pre-crRNA into individual
CC crRNA units, in vivo at least Cas5 and Cas7 are also required for
CC optimal crRNA processing and/or stability (PubMed:24459147).
CC {ECO:0000269|PubMed:22767603, ECO:0000269|PubMed:24459147}.
CC -!- SUBUNIT: Component of the Cascade-like complex (Cascade I-B), composed
CC of Cas5, Cas6, Cas7 and crRNA. {ECO:0000269|PubMed:24459147}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:24459147}.
CC -!- INDUCTION: Expressed in exponential and stationary phases.
CC {ECO:0000269|PubMed:24459147}.
CC -!- DISRUPTION PHENOTYPE: Loss of the 8 Cas genes in this locus (cas1,
CC cas2, cas3, cas4, cas5, cas6, cas7 and cas8b) leads to loss of CRISPR
CC interference against plasmid targeted by this CRISPR locus, i.e.
CC plasmid is not destroyed by CRISPR (PubMed:22767603). Upon disruption
CC of only this gene, no visible phenotype when grown under differing
CC temperature and salt regimes. Does not produce mature length crRNA. Can
CC be complemented by endogenous Cas6, but not by Cas6 from halophilic
CC archaea H.lacusprofundi, or non-halophilic archaea M. maripaludis or M.
CC mazei. Loss of CRISPR interference against plasmid targeted by this
CC CRISPR locus, i.e. plasmid is not destroyed by CRISPR action
CC (PubMed:24459147). {ECO:0000269|PubMed:22767603,
CC ECO:0000269|PubMed:24459147}.
CC -!- MISCELLANEOUS: There are 3 CRISPR RNA loci in this organism and a
CC single cas gene locus. A CRISPR-Cas type I-B system.
CC {ECO:0000303|PubMed:20333302, ECO:0000303|PubMed:24459147}.
CC -!- SIMILARITY: Belongs to the CRISPR-associated protein Cas6/Cse3/CasE
CC family. {ECO:0000305}.
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DR EMBL; CP001955; ADE01675.1; -; Genomic_DNA.
DR RefSeq; WP_013035014.1; NZ_AOHU01000052.1.
DR AlphaFoldDB; D4GQN4; -.
DR SMR; D4GQN4; -.
DR STRING; 309800.C498_09701; -.
DR EnsemblBacteria; ADE01675; ADE01675; HVO_A0205.
DR GeneID; 8923584; -.
DR KEGG; hvo:HVO_A0205; -.
DR eggNOG; arCOG04342; Archaea.
DR HOGENOM; CLU_089858_1_0_2; -.
DR OMA; NIGEMPF; -.
DR OrthoDB; 59332at2157; -.
DR Proteomes; UP000008243; Plasmid pHV4.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR InterPro; IPR010156; CRISPR-assoc_prot_Cas6.
DR Pfam; PF01881; Cas_Cas6; 1.
PE 1: Evidence at protein level;
KW Antiviral defense; Cytoplasm; Endonuclease; Hydrolase; Nuclease; Plasmid;
KW Reference proteome; RNA-binding.
FT CHAIN 1..272
FT /note="CRISPR-associated endoribonuclease Cas6"
FT /id="PRO_0000432153"
FT ACT_SITE 41
FT /note="Proton donor"
FT /evidence="ECO:0000305|PubMed:24459147"
FT MUTAGEN 41
FT /note="H->A: About 40% of mature crRNA produced, 20%
FT reduced CRISPR plasmid interference."
FT /evidence="ECO:0000269|PubMed:24459147"
FT MUTAGEN 115
FT /note="S->A: About 130% of mature crRNA produced, CRISPR
FT plasmid interference restored in a deletion mutant."
FT /evidence="ECO:0000269|PubMed:24459147"
FT MUTAGEN 224
FT /note="S->A: About 160% of mature crRNA produced, CRISPR
FT plasmid interference restored in a deletion mutant."
FT /evidence="ECO:0000269|PubMed:24459147"
FT MUTAGEN 256
FT /note="G->A: About 10% of mature crRNA produced, no CRISPR
FT plasmid interefence observed."
FT /evidence="ECO:0000269|PubMed:24459147"
FT MUTAGEN 258
FT /note="G->A: No mature crRNA produced, no CRISPR plasmid
FT interefence observed."
FT /evidence="ECO:0000269|PubMed:24459147"
SQ SEQUENCE 272 AA; 31051 MW; 357C604A701DC4B0 CRC64;
MRIELALDAV ADAAYDRSYF HKARGRIWRG LENDDTFSET HDSNHGVGFS YSNIFPWGQI
EEGDRRYFRI ASPRRGLLDT LIAHFGRERA FEIGQMRFTI DDITGHAPDV GEAGSTGVLE
TGTGVHCALG RQLAEEYNLD TSKIDAGESE TELFWRPKHG MEPLQDAIRR SLQQTHELYG
DDYYDGPGEV DEPLFDRIEP IKDDVVYPVR FQPATAVDRT VLLSKWRFGY RVRDETHRYH
LNLALDSGIG QRREHGFGFL NLTDKTLPRA SD
