ID   Y5234_MYCSE             Reviewed;         481 AA.
AC   L8F618; A0A2U9PW47; B3GNJ4;
DT   30-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT   03-APR-2013, sequence version 1.
DT   25-MAY-2022, entry version 28.
DE   RecName: Full=Uncharacterized protein D806_5234;
GN   ORFNames=5168d {ECO:0000303|PubMed:18554329},
GN   D806_050620 {ECO:0000312|EMBL:AWT56012.1}, D806_5234;
OS   Mycolicibacterium smegmatis (strain MKD8) (Mycobacterium smegmatis).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycolicibacterium.
OX   NCBI_TaxID=1214915;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=MKD8;
RX   PubMed=18554329; DOI=10.1111/j.1365-2958.2008.06299.x;
RA   Coros A., Callahan B., Battaglioli E., Derbyshire K.M.;
RT   "The specialized secretory apparatus ESX-1 is essential for DNA transfer in
RT   Mycobacterium smegmatis.";
RL   Mol. Microbiol. 69:794-808(2008).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MKD8;
RX   PubMed=23618714; DOI=10.1128/genomea.00148-13;
RA   Gray T.A., Palumbo M.J., Derbyshire K.M.;
RT   "Draft genome sequence of MKD8, a conjugal recipient Mycobacterium
RT   smegmatis strain.";
RL   Genome Announc. 1:E0014813-E0014813(2013).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MKD8;
RA   Derbyshire K., Gray T.A., Champion M.;
RL   Submitted (MAR-2018) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Involved in DNA conjugation in the recipient strain
CC       (PubMed:18554329). {ECO:0000269|PubMed:18554329}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255}; Single-pass
CC       membrane protein {ECO:0000255}.
CC   -!- DISRUPTION PHENOTYPE: Loss of DNA conjugation when disrupted in
CC       recipient strain, strain still secretes EsxB (PubMed:18554329).
CC       {ECO:0000269|PubMed:18554329}.
CC   -!- MISCELLANEOUS: In the well characterized mc(2)155 strain this gene not
CC       present; it is part of an MKD8 strain-specific insert (RSR-II) between
CC       MSMEG_5168 and MSMEG_5169. {ECO:0000305}.
CC   -!- MISCELLANEOUS: DNA conjugation in M.smegmatis is unidirectional with
CC       distinct donor and recipient strains; mc(2)155 is a donor strain while
CC       MKD8 is a recipient strain. Mutations in a donor strain that alter DNA
CC       transfer do not always alter DNA transfer in a recipient strain.
CC       {ECO:0000305|PubMed:18554329}.
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DR   EMBL; EU711430; ACE06968.1; -; Genomic_DNA.
DR   EMBL; CP027541; AWT56012.1; -; Genomic_DNA.
DR   AlphaFoldDB; L8F618; -.
DR   EnsemblBacteria; AWT56012; AWT56012; D806_050620.
DR   PATRIC; fig|1214915.3.peg.5259; -.
DR   HOGENOM; CLU_049593_0_0_11; -.
DR   Proteomes; UP000011200; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Membrane; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..481
FT                   /note="Uncharacterized protein D806_5234"
FT                   /id="PRO_0000438357"
FT   TRANSMEM        18..38
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   481 AA;  51830 MW;  C2CDA4D5B6C4B6AA CRC64;
     MIIDSKGRRI IPPSARRFMI VTAIAVAIFV VITGVVIFVT RPPTSDSAAT FHRGDWQAPP
     EALLSSPMRV RPVPGWRVRM TDLGLPESAV FADTELTHEA EPFVGAMEKR AYFMAMSTTD
     PRRWLVGLDL STGRPLFSPV SIDPGPDFVK CFVNGPDQVL CAADGKPEGK TATIAWVVDT
     RTGEVVFNGP TDLHVTPGSG SHLKQVGSYV VAEIQGKGLS GVGPRAETTW LIPDAKKVTP
     TNRNADTAAP RLAVAEDLAG GPDHAVVFSV VDGTVITPDL GADRTPERAV VYPQGFAILA
     AGKRGSGLRD TVLFFDNAGN RVGESGIQGS LSDLSMVLPM VQSAPSYTVF GANGAGLIQL
     PGEGLDAVLI GHRLYAPESE WDGPVKVRRW RQFDLRTGEE GTACLPNMQW YIANDGQVGV
     FETSQHETTG ATFFGMDLTT CEKLWTVPVN WESFHRLWRI DDTLVELSDD GKELHSLVAP
     A