ID   CAS6_MYXXD              Reviewed;         230 AA.
AC   Q1CW45;
DT   11-JUL-2012, integrated into UniProtKB/Swiss-Prot.
DT   11-JUL-2006, sequence version 1.
DT   03-AUG-2022, entry version 83.
DE   RecName: Full=CRISPR-associated endonuclease Cas6;
DE            EC=3.1.-.-;
GN   Name=cas6; OrderedLocusNames=MXAN_7265;
OS   Myxococcus xanthus (strain DK1622).
OC   Bacteria; Proteobacteria; Deltaproteobacteria; Myxococcales;
OC   Cystobacterineae; Myxococcaceae; Myxococcus.
OX   NCBI_TaxID=246197;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DK1622;
RX   PubMed=17015832; DOI=10.1073/pnas.0607335103;
RA   Goldman B.S., Nierman W.C., Kaiser D., Slater S.C., Durkin A.S., Eisen J.,
RA   Ronning C.M., Barbazuk W.B., Blanchard M., Field C., Halling C., Hinkle G.,
RA   Iartchuk O., Kim H.S., Mackenzie C., Madupu R., Miller N., Shvartsbeyn A.,
RA   Sullivan S.A., Vaudin M., Wiegand R., Kaplan H.B.;
RT   "Evolution of sensory complexity recorded in a myxobacterial genome.";
RL   Proc. Natl. Acad. Sci. U.S.A. 103:15200-15205(2006).
RN   [2]
RP   INDUCTION, AND OPERON STRUCTURE.
RC   STRAIN=DK1622;
RX   PubMed=17369305; DOI=10.1128/jb.00187-07;
RA   Viswanathan P., Murphy K., Julien B., Garza A.G., Kroos L.;
RT   "Regulation of dev, an operon that includes genes essential for Myxococcus
RT   xanthus development and CRISPR-associated genes and repeats.";
RL   J. Bacteriol. 189:3738-3750(2007).
CC   -!- FUNCTION: CRISPR (clustered regularly interspaced short palindromic
CC       repeat) is an adaptive immune system that provides protection against
CC       mobile genetic elements (viruses, transposable elements and conjugative
CC       plasmids). CRISPR clusters contain sequences complementary to
CC       antecedent mobile elements and target invading nucleic acids. CRISPR
CC       clusters are transcribed and processed into CRISPR RNA (crRNA).
CC       Processes pre-crRNA into individual crRNA units (By similarity).
CC       {ECO:0000250}.
CC   -!- DEVELOPMENTAL STAGE: Operon expression begins by 6 hours after
CC       starvation has initiated development and is under strong negative
CC       regulation by DevS.
CC   -!- INDUCTION: Part of an operon going from at least MXAN_7266 to MXAN_7259
CC       that includes a CRISPR operon with transcription continuing into the
CC       pre-crRNA locus. {ECO:0000269|PubMed:17369305}.
CC   -!- SIMILARITY: Belongs to the CRISPR-associated protein Cas6/Cse3/CasE
CC       family. Subtype Myxan subfamily. {ECO:0000305}.
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DR   EMBL; CP000113; ABF87577.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q1CW45; -.
DR   STRING; 246197.MXAN_7265; -.
DR   EnsemblBacteria; ABF87577; ABF87577; MXAN_7265.
DR   KEGG; mxa:MXAN_7265; -.
DR   eggNOG; ENOG502Z8Q5; Bacteria.
DR   HOGENOM; CLU_096068_1_0_7; -.
DR   OMA; QNGWERP; -.
DR   Proteomes; UP000002402; Chromosome.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR   CDD; cd09703; Cas6-I-III; 1.
DR   InterPro; IPR014174; CRISPR-assoc_prot_Cas6-rel.
DR   Pfam; PF09559; Cas6; 1.
DR   TIGRFAMs; TIGR02807; cas6_cmx6; 1.
PE   2: Evidence at transcript level;
KW   Antiviral defense; Endonuclease; Hydrolase; Nuclease; Reference proteome;
KW   RNA-binding.
FT   CHAIN           1..230
FT                   /note="CRISPR-associated endonuclease Cas6"
FT                   /id="PRO_0000418223"
SQ   SEQUENCE   230 AA;  24876 MW;  66DB720E4777D92E CRC64;
     MARSVGWRRS ALITVSGCVI LNWWGNLMVF VDLLFPVQGG PVPLDHAYLL FSALSRHLPA
     LHERSDMGVF SLRGVSNTRE LLYLGRGTMR LRCPIEAVAT LLPLVSAPLE IAGRRLSLGA
     PSLHALEPVP SLFARLVTFK HAMDEAAFVA AASRALEALG VQATLKVGRR RIVRITGKKV
     VGFALELHGL SAEHSLRVQE QGMGGRRHMG CGLFLPPGRA ARVQSHGKAA